Genes for Autoregulation of Nodulation

2014 ◽  
pp. 73-78
Author(s):  
Masayoshi Kawaguchi
Keyword(s):  
Autoregulation Of Nodulation

Differential responses of the sunn4 and rdn1-1 super-nodulation mutants of Medicago truncatula to elevated atmospheric CO2

2021 ◽  
Author(s):  
Yunfa Qiao ◽  
Shujie Miao ◽  
Jian Jin ◽  
Ulrike Mathesius ◽  
Caixian Tang
Keyword(s):  
Nitrogen Fixation ◽  
Elevated Co2 ◽  
Medicago Truncatula ◽  
N2 Fixation ◽  
Sink Strength ◽  
Wild Type ◽  
Total N ◽  
Autoregulation Of Nodulation ◽  
Nodulation Mutants ◽  
Fixation Capacity

Abstract Background and Aims Nitrogen fixation in legumes requires tight control of carbon and nitrogen balance. Thus, legumes control nodule numbers via an autoregulation mechanism. ‘Autoregulation of nodulation’ mutants super-nodulate and are thought to be carbon-limited due to the high carbon-sink strength of excessive nodules. This study aimed to examine the effect of increasing carbon supply on the performance of super-nodulation mutants. Methods We compared the responses of Medicago truncatula super-nodulation mutants (sunn-4 and rdn1-1) and wild type to five CO2 levels (300-850 μmol mol -1). Nodule formation and N2 fixation were assessed in soil-grown plants at 18 and 42 days after sowing. Key results Shoot and root biomass, nodule number and biomass, nitrogenase activity and fixed-N per plant of all genotypes increased with increasing CO2 concentration and reached the maximum around 700 μmol mol -1. While the sunn-4 mutant showed strong growth-retardation compared to wild-type plants, elevated CO2 increased shoot biomass and total N content of rdn1-1 mutant up to two-fold. This was accompanied by a four-fold increase in nitrogen fixation capacity in the rdn1-1 mutant. Conclusions These results suggest that the super-nodulation phenotype per se did not limit growth. The additional nitrogen fixation capacity of the rdn1-1 mutant may enhance the benefit of elevated CO2 on plant growth and N2 fixation.

scholarly journals Characterisation of Medicago truncatula CLE34 and CLE35 in nodulation control

2020 ◽  
Author(s):  
Celine Mens ◽  
April H. Hastwell ◽  
Huanan Su ◽  
Peter M. Gresshoff ◽  
Ulrike Mathesius ◽  
...  
Keyword(s):  
Pisum Sativum ◽  
Medicago Truncatula ◽  
Sequence Homology ◽  
Dependent Manner ◽  
Legume Species ◽  
Mature Peptide ◽  
Cle Peptides ◽  
Autoregulation Of Nodulation ◽  
Cle Peptide ◽  
Distinct Role

AbstractLegume plants form a symbiosis with N2-fixing soil rhizobia, resulting in new root organs called nodules that enable N2-fixation. Nodulation is a costly process that is tightly regulated by the host through Autoregulation of Nodulation (AON) and nitrate-dependent regulation of nodulation. Both pathways require legume-specific CLAVATA/ESR-related (CLE) peptides. Nitrogen-induced nodulation-suppressing CLE peptides have not previously been characterised in Medicago truncatula, with only rhizobia-induced MtCLE12 and MtCLE13 identified. Here, we report on novel peptides MtCLE34 and MtCLE35 in nodulation control pathways. The nodulation-suppressing CLE peptides of five legume species were classified into three clades based on sequence homology and phylogeny. This approached identified MtCLE34 and MtCLE35 and four new CLE peptide orthologues of Pisum sativum. Whereas MtCLE12 and MtCLE13 are induced by rhizobia, MtCLE34 and MtCLE35 respond to both rhizobia and nitrate. MtCLE34 was identified as a pseudogene lacking a functional CLE-domain. Overexpression of MtCLE12, MtCLE13 and MtCLE35 inhibits nodulation. Together, our findings indicate that MtCLE12 and MtCLE13 have a distinct role in AON, while MtCLE35 regulates nodule numbers in a rhizobia- and nitrate-dependent manner. MtCLE34 likely had a similar role to MtCLE35 but its function was lost due to a nonsense mutation resulting in the loss of the mature peptide.

scholarly journals CLE peptide tri-arabinosylation and peptide domain sequence composition are essential for SUNN-dependent autoregulation of nodulation inMedicago truncatula

2018 ◽  
Vol 218 (1) ◽  
pp. 73-80 ◽  
Author(s):  
Nijat Imin ◽  
Neha Patel ◽  
Leo Corcilius ◽  
Richard J. Payne ◽  
Michael A. Djordjevic
Keyword(s):  
Sequence Composition ◽  
Domain Sequence ◽  
Autoregulation Of Nodulation ◽  
Cle Peptide

Inhibition of legume nodulation by Pi deficiency is dependent on the autoregulation of nodulation (AON) pathway

2020 ◽  
Vol 103 (3) ◽  
pp. 1125-1139
Author(s):  
Mariel C. Isidra‐Arellano ◽  
Eithan A. Pozas‐Rodríguez ◽  
María Rocío Reyero‐Saavedra ◽  
Jazmin Arroyo‐Canales ◽  
Susana Ferrer‐Orgaz ◽  
...  
Keyword(s):  
Legume Nodulation ◽  
Autoregulation Of Nodulation ◽  
Pi Deficiency

scholarly journals Regulation of Resource Partitioning Coordinates Nitrogen and Rhizobia Responses and Autoregulation of Nodulation in Medicago truncatula

2019 ◽  
Vol 12 (6) ◽  
pp. 833-846 ◽  
Author(s):  
Beatriz Lagunas ◽  
Mingkee Achom ◽  
Roxanna Bonyadi-Pour ◽  
Alonso J. Pardal ◽  
Bethany L. Richmond ◽  
...  
Keyword(s):  
Medicago Truncatula ◽  
Resource Partitioning ◽  
Autoregulation Of Nodulation

Identification of tissue-specific gene clusters and orthologues of nodulation-related genes in Vigna angularis

2014 ◽  
Vol 12 (S1) ◽  
pp. S21-S26 ◽  
Author(s):  
Yang Jae Kang ◽  
Jayern Lee ◽  
Yong Hwan Kim ◽  
Suk-Ha Lee
Keyword(s):  
De Novo ◽  
Transcriptome Assembly ◽  
Lotus Japonicus ◽  
Gene Clusters ◽  
Specific Gene ◽  
Vigna Angularis ◽  
Model Legume ◽  
Tissue Specific ◽  
Autoregulation Of Nodulation ◽  
Species Specific

Nitrogen fixation in legumes is an important agricultural trait that results from symbiosis between the root and rhizobia. To understand the molecular basis of nodulation, recent research has been focused on the identification of nodulation-related genes by functional analysis using two major model legumes, Medicago truncatula and Lotus japonicus. Thus far, three important processes have been discovered, namely Nod factor (NF) perception, NF signalling and autoregulation of nodulation. Nevertheless, application of the results of these studies is limited for non-model legume crops because a reference genome is unavailable. However, because the cost of whole-transcriptome analysis has dropped dramatically due to the Next generation sequencer (NGS) technology, minor crops for which reference sequences are yet to be constructed can still be studied at the genome level. In this study, we sequenced the leaf and root transcriptomes of Vigna angularis (accession IT213134) and de novo assembled. Our results demonstrate the feasibility of using the transcriptome assembly to effectively identify tissue-specific peptide clusters related to tissue-specific functions and species-specific nodulation-related genes.

scholarly journals Molecular mechanisms controlling legume autoregulation of nodulation

2011 ◽  
Vol 108 (5) ◽  
pp. 789-795 ◽  
Author(s):  
Dugald E. Reid ◽  
Brett J. Ferguson ◽  
Satomi Hayashi ◽  
Yu-Hsiang Lin ◽  
Peter M. Gresshoff
Keyword(s):  
Molecular Mechanisms ◽  
Autoregulation Of Nodulation

scholarly journals A functional–structural modelling approach to autoregulation of nodulation

2010 ◽  
Vol 107 (5) ◽  
pp. 855-863 ◽  
Author(s):  
Liqi Han ◽  
Peter M. Gresshoff ◽  
Jim Hanan
Keyword(s):  
Structural Modelling ◽  
Autoregulation Of Nodulation ◽  
Modelling Approach

Legume nodulation: successful symbiosis through short- and long-distance signalling

2006 ◽  
Vol 33 (8) ◽  
pp. 707 ◽  
Author(s):  
Mark Kinkema ◽  
Paul T. Scott ◽  
Peter M. Gresshoff
Keyword(s):  
Molecular Genetic ◽  
Signal Transduction Pathway ◽  
Molecular Genetic Analysis ◽  
Long Distance ◽  
Symbiotic Interaction ◽  
Available Nitrogen ◽  
Autoregulation Of Nodulation ◽  
Systemic Signal ◽  
Review Reports ◽  
Level Of Understanding

Nodulation in legumes provides a major conduit of available nitrogen into the biosphere. The development of nitrogen-fixing nodules results from a symbiotic interaction between soil bacteria, commonly called rhizobia, and legume plants. Molecular genetic analysis in both model and agriculturally important legume species has resulted in the identification of a variety of genes that are essential for the establishment, maintenance and regulation of this symbiosis. Autoregulation of nodulation (AON) is a major internal process by which nodule numbers are controlled through prior nodulation events. Characterisation of AON-deficient mutants has revealed a novel systemic signal transduction pathway controlled by a receptor-like kinase. This review reports our present level of understanding on the short- and long-distance signalling networks controlling early nodulation events and AON.

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