Functional Analysis of Sugar Chains Using a Genome-Wide RNAi System in Drosophila

2009 ◽  
pp. 285-289
Author(s):  
Shoko Nishihara
Keyword(s):  
Functional Analysis ◽  
Genome Wide ◽  
A Genome ◽  
Sugar Chains

scholarly journals A Genome-Wide mRNA Screen and Functional Analysis Reveal FOXO3 as a Candidate Gene for Chicken Growth

PLoS ONE ◽  
2015 ◽  
Vol 10 (9) ◽  
pp. e0137087 ◽  
Author(s):  
Biao Chen ◽  
Jiguo Xu ◽  
Xiaomei He ◽  
Haiping Xu ◽  
Guihuan Li ◽  
...  
Keyword(s):  
Functional Analysis ◽  
Candidate Gene ◽  
Genome Wide ◽  
A Genome

scholarly journals Genome-wide identification of diacylglycerol acyltransferases (DGAT) family genes influencing milk production traits in buffalo

2020 ◽  
Author(s):  
Jiajia Liu ◽  
Zhiquan Wang ◽  
Jun Li ◽  
Hui Li ◽  
Liguo Yang
Keyword(s):  
Functional Analysis ◽  
Milk Production ◽  
Milk Fat ◽  
Buffalo Milk ◽  
Production Traits ◽  
Lactation Performance ◽  
Milk Production Traits ◽  
Chromosomal Distribution ◽  
Genome Wide ◽  
A Genome

Abstract Background: The diacylglycerol acyltransferases (DGAT) are a vital group of enzymes in catalyzing triacylglycerol biosynthesis. DGAT genes like DGAT1 and DGAT2 , have been identified as two functional candidate genes affecting milk production traits, especially for fat content in milk. Buffalo milk is famous for its excellent quality, which is rich in fat and protein content. Therefore, this study aimed to characterize DGAT family genes in buffalo and to find candidate markers or DGAT genes influencing lactation performance. Results: We performed a genome-wide study and identified eight DGAT genes in buffalo. All the DGAT genes classified into two distinct clades (DGAT1 and DGAT2 subfamily) based on their phylogenetic relationships and structural features. Chromosome localization displayed eight buffalo DGAT genes distributed on five chromosomes. Collinearity analysis revealed that the DGAT family genes were extensive homologous between buffalo and cattle. Afterward, we discovered genetic variants loci within the genomic regions that DGAT genes located in buffalo. Seven haplotype blocks were constructed and were associated with buffalo milk production traits. Single marker association analyses revealed four most significant single nucleotide polymorphisms (SNPs) mainly affecting milk protein percentage or milk fat yield in buffalo. Genes functional analysis indicated that these DGAT family genes could influence lactation performance in the mammal through regulating lipid metabolism. Conclusion: In the present study, we performed a comprehensive analysis for the DGAT family genes in buffalo, which including identification, structural characterization, phylogenetic classification, chromosomal distribution, collinearity analysis, association analysis, and functional analysis. These findings provide useful information for an in-depth study to determine the role of DGAT family gens play in the regulation of milk production and milk quality improvement in buffalo.

scholarly journals Genome-wide identification of diacylglycerol acyltransferases (DGAT) family genes influencing milk production traits in buffalo

2020 ◽  
Author(s):  
Jiajia Liu ◽  
Zhiquan Wang ◽  
Jun Li ◽  
Hui Li ◽  
Liguo Yang
Keyword(s):  
Functional Analysis ◽  
Milk Production ◽  
Milk Fat ◽  
Buffalo Milk ◽  
Production Traits ◽  
Lactation Performance ◽  
Milk Production Traits ◽  
Chromosomal Distribution ◽  
Genome Wide ◽  
A Genome

Abstract Background: The diacylglycerol acyltransferases (DGAT) are a vital group of enzymes in catalyzing triacylglycerol biosynthesis. DGAT genes like DGAT1 and DGAT2 , have been identified as two functional candidate genes affecting milk production traits, especially for fat content in milk. Buffalo milk is famous for its excellent quality, which is rich in fat and protein content. Therefore, this study aimed to characterize DGAT family genes in buffalo and to find candidate markers or DGAT genes influencing lactation performance. Results: We performed a genome-wide study and identified eight DGAT genes in buffalo. All the DGAT genes classified into two distinct clades (DGAT1 and DGAT2 subfamily) based on their phylogenetic relationships and structural features. Chromosome localization displayed eight buffalo DGAT genes distributed on five chromosomes. Collinearity analysis revealed that the DGAT family genes were extensive homologous between buffalo and cattle. Afterward, we discovered genetic variants loci within the genomic regions that DGAT genes located in buffalo. Seven haplotype blocks were constructed and were associated with buffalo milk production traits. Single marker association analyses revealed four most significant single nucleotide polymorphisms (SNPs) mainly affecting milk protein percentage or milk fat yield in buffalo. Genes functional analysis indicated that these DGAT family genes could influence lactation performance in the mammal through regulating lipid metabolism. Conclusion: In the present study, we performed a comprehensive analysis for the DGAT family genes in buffalo, which including identification, structural characterization, phylogenetic classification, chromosomal distribution, collinearity analysis, association analysis, and functional analysis. These findings provide useful information for an in-depth study to determine the role of DGAT family gens play in the regulation of milk production and milk quality improvement in buffalo.

scholarly journals Genome-wide identification of diacylglycerol acyltransferases (DGAT) family genes influencing milk production traits in buffalo

2019 ◽  
Author(s):  
Jiajia Liu ◽  
Zhiquan Wang ◽  
Jun Li ◽  
Hui Li ◽  
Liguo Yang
Keyword(s):  
Functional Analysis ◽  
Milk Production ◽  
Milk Fat ◽  
Buffalo Milk ◽  
Production Traits ◽  
Lactation Performance ◽  
Milk Production Traits ◽  
Chromosomal Distribution ◽  
Genome Wide ◽  
A Genome

Abstract Background: The diacylglycerol acyltransferases (DGAT) are a vital group of enzymes in catalyzing triacylglycerol biosynthesis. DGAT genes like DGAT1 and DGAT2 , have been identified as two functional candidate genes affecting milk production traits, especially for fat content in milk. Buffalo milk is famous for its excellent quality, which is rich in fat and protein content. Therefore, this study aimed to characterize DGAT family genes in buffalo and to find candidate markers or DGAT genes influencing lactation performance. Results: We performed a genome-wide study and identified eight DGAT genes in buffalo. All the DGAT genes classified into two distinct clades (DGAT1 and DGAT2 subfamily) based on their phylogenetic relationships and structural features. Chromosome localization displayed eight buffalo DGAT genes distributed on five chromosomes. Collinearity analysis revealed that the DGAT family genes were extensive homologous between buffalo and cattle. Afterward, we discovered genetic variants loci within the genomic regions that DGAT genes located in buffalo. Seven haplotype blocks were constructed and were associated with buffalo milk production traits. Single marker association analyses revealed four most significant single nucleotide polymorphisms (SNPs) mainly affecting milk protein percentage or milk fat yield in buffalo. Genes functional analysis indicated that these DGAT family genes could influence lactation performance in the mammal through regulating lipid metabolism. Conclusion: In the present study, we performed a comprehensive analysis for the DGAT family genes in buffalo, which including identification, structural characterization, phylogenetic classification, chromosomal distribution, collinearity analysis, association analysis, and functional analysis. These findings provide useful information for an in-depth study to determine the role of DGAT family gens play in the regulation of milk production and milk quality improvement in buffalo.

scholarly journals Identification of the Wnt signaling activator leucine-rich repeat in Flightless interaction protein 2 by a genome-wide functional analysis

2005 ◽  
Vol 102 (6) ◽  
pp. 1927-1932 ◽  
Author(s):  
J. Liu ◽  
A. G. Bang ◽  
C. Kintner ◽  
A. P. Orth ◽  
S. K. Chanda ◽  
...  
Keyword(s):  
Functional Analysis ◽  
Wnt Signaling ◽  
Leucine Rich Repeat ◽  
Genome Wide ◽  
A Genome

scholarly journals Human leucine-rich repeat proteins: a genome-wide bioinformatic categorization and functional analysis in innate immunity

2010 ◽  
Vol 108 (Supplement_1) ◽  
pp. 4631-4638 ◽  
Author(s):  
A. C. Y. Ng ◽  
J. M. Eisenberg ◽  
R. J. W. Heath ◽  
A. Huett ◽  
C. M. Robinson ◽  
...  
Keyword(s):  
Functional Analysis ◽  
Innate Immunity ◽  
Leucine Rich Repeat ◽  
Repeat Proteins ◽  
Genome Wide ◽  
A Genome

scholarly journals Genome-wide functional analysis reveals that infection-associated fungal autophagy is necessary for rice blast disease

2009 ◽  
Vol 106 (37) ◽  
pp. 15967-15972 ◽  
Author(s):  
Michael J. Kershaw ◽  
Nicholas J. Talbot
Keyword(s):  
Functional Analysis ◽  
Rice Blast ◽  
Rice Blast Disease ◽  
Blast Disease ◽  
Deletion Mutants ◽  
Targeted Deletion ◽  
Genome Wide Analysis ◽  
Plant Infection ◽  
Genome Wide ◽  
A Genome

To cause rice blast disease, the fungus Magnaporthe oryzae elaborates specialized infection structures called appressoria, which use enormous turgor to rupture the tough outer cuticle of a rice leaf. Here, we report the generation of a set of 22 isogenic M. oryzae mutants each differing by a single component of the predicted autophagic machinery of the fungus. Analysis of this set of targeted deletion mutants demonstrated that loss of any of the 16 genes necessary for nonselective macroautophagy renders the fungus unable to cause rice blast disease, due to impairment of both conidial programmed cell death and appressorium maturation. In contrast, genes necessary only for selective forms of autophagy, such as pexophagy and mitophagy, are dispensable for appressorium-mediated plant infection. A genome-wide analysis therefore demonstrates the importance of infection-associated, nonselective autophagy for the establishment of rice blast disease.

scholarly journals Genome-wide identification of diacylglycerol acyltransferases (DGAT) family genes influencing milk production traits in buffalo

2019 ◽  
Author(s):  
Jiajia Liu ◽  
Zhiquan Wang ◽  
Jun Li ◽  
Hui Li ◽  
Liguo Yang
Keyword(s):  
Functional Analysis ◽  
Milk Production ◽  
Milk Fat ◽  
Buffalo Milk ◽  
Production Traits ◽  
Lactation Performance ◽  
Milk Production Traits ◽  
Chromosomal Distribution ◽  
Genome Wide ◽  
A Genome

Abstract Background: The diacylglycerol acyltransferases (DGAT) are a vital group of enzymes in catalyzing triacylglycerol biosynthesis. DGAT genes like DGAT1 and DGAT2 , have been identified as two functional candidate genes affecting milk production traits, especially for fat content in milk. Buffalo milk is famous for its excellent quality, which is rich in fat and protein content. Therefore, this study aimed to characterize DGAT family genes in buffalo and to find candidate markers or DGAT genes influencing lactation performance. Results: We performed a genome-wide detection and identified eight DGAT genes in buffalo. All the DGAT genes classified into two distinct clades (DGAT1 and DGAT2 subfamily) based on their phylogenetic relationships and structural features. Chromosome localization displayed eight buffalo DGAT genes distributed on five chromosomes. Collinearity analysis revealed that the DGAT family genes were extensive homologous between buffalo and cattle. Afterward, we discovered genetic variants loci within the genomic regions that DGAT genes located in buffalo. Eight haplotype blocks were constructed and were associated with buffalo milk production traits. Single marker association analyses revealed four most significant single nucleotide polymorphisms (SNPs) mainly affecting milk protein percentage or milk fat yield in buffalo. Genes functional analysis indicated that these DGAT family genes could influence lactation performance in the mammal through regulating lipid metabolism. Conclusion: In the present study, we performed a comprehensive analysis for the DGAT family genes in buffalo, which including identification, structural characterization, phylogenetic classification, chromosomal distribution, collinearity analysis, association analysis, and functional analysis. These findings provide useful information for an in-depth study to determine the role of DGAT family gens play in the regulation of milk production and milk quality improvement in buffalo. Keywords: DGAT family, DGAT1, DGAT2 , Milk production traits, Buffalo genome

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