PCR Amplification, Sequencing, and In Silico Characterization of Pectin Lyase Genes from Aspergillus flavus NIICC8142

2014 ◽  
pp. 413-421
Author(s):  
Amit Kumar Dubey ◽  
Sangeeta Yadav ◽  
Gautam Anand ◽  
Dinesh Yadav
Keyword(s):  
Aspergillus Flavus ◽  
In Silico ◽  
Pcr Amplification ◽  
Pectin Lyase ◽  
In Silico Characterization

IN VITRO/IN SILICO CHARACTERIZATION OF ACTIVE AND PASSIVE STRESSES IN CARDIAC MUSCLE

2012 ◽  
Vol 10 (2) ◽  
pp. 171-188 ◽  
Author(s):  
Markus Boel ◽  
Oscar J. Abilez ◽  
Ahmed N Assar ◽  
Christopher K. Zarins ◽  
Ellen Kuhl
Keyword(s):  
Cardiac Muscle ◽  
In Silico ◽  
In Silico Characterization

In-Vitro and In-Silico Characterization of Xylose Reductase from Emericella nidulans

2019 ◽  
Vol 13 (2) ◽  
pp. 159-170 ◽  
Author(s):  
Vishal Ahuja ◽  
Aashima Sharma ◽  
Ranju Kumari Rathour ◽  
Vaishali Sharma ◽  
Nidhi Rana ◽  
...  
Keyword(s):  
Production Process ◽  
In Silico ◽  
Xylose Reductase ◽  
In Silico Analysis ◽  
Emericella Nidulans ◽  
Higher Temperature ◽  
In Silico Characterization ◽  
Silico Analysis

Background: Lignocellulosic residues generated by various anthropogenic activities can be a potential raw material for many commercial products such as biofuels, organic acids and nutraceuticals including xylitol. Xylitol is a low-calorie nutritive sweetener for diabetic patients. Microbial production of xylitol can be helpful in overcoming the drawbacks of traditional chemical production process and lowring cost of production. Objective: Designing efficient production process needs the characterization of required enzyme/s. Hence current work was focused on in-vitro and in-silico characterization of xylose reductase from Emericella nidulans. Methods: Xylose reductase from one of the hyper-producer isolates, Emericella nidulans Xlt-11 was used for in-vitro characterization. For in-silico characterization, XR sequence (Accession No: Q5BGA7) was used. Results: Xylose reductase from various microorganisms has been studied but the quest for better enzymes, their stability at higher temperature and pH still continues. Xylose reductase from Emericella nidulans Xlt-11 was found NADH dependent and utilizes xylose as its sole substrate for xylitol production. In comparison to whole cells, enzyme exhibited higher enzyme activity at lower cofactor concentration and could tolerate higher substrate concentration. Thermal deactivation profile showed that whole cell catalysts were more stable than enzyme at higher temperature. In-silico analysis of XR sequence from Emericella nidulans (Accession No: Q5BGA7) suggested that the structure was dominated by random coiling. Enzyme sequences have conserved active site with net negative charge and PI value in acidic pH range. Conclusion: Current investigation supported the enzyme’s specific application i.e. bioconversion of xylose to xylitol due to its higher selectivity. In-silico analysis may provide significant structural and physiological information for modifications and improved stability.

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ChemMedChem ◽  
2021 ◽  
Author(s):  
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Diego I. Figueroa-Figueroa ◽  
Francisco Hernández-Luis ◽  
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Keyword(s):  
In Silico ◽  
Main Protease ◽  
In Silico Characterization

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2021 ◽  
pp. 1-16
Author(s):  
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Shravan B. Rathod ◽  
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Priya Sharma ◽  
Lata Nebhnani ◽  
...  
Keyword(s):  
In Silico ◽  
Structural Proteins ◽  
In Silico Characterization

Effect and in silico characterization of genetic variants associated with severe spermatogenic disorders in a large Iberian cohort

Andrology ◽  
2021 ◽  
Author(s):  
Miriam Cerván‐Martín ◽  
Lara Bossini‐Castillo ◽  
Rocío Rivera‐Egea ◽  
Nicolás Garrido ◽  
Saturnino Luján ◽  
...  
Keyword(s):  
Genetic Variants ◽  
In Silico ◽  
In Silico Characterization

scholarly journals Genome-wide identification and in silico characterization of chitinase gene family in Foxtail millet (Setaria italica)

2020 ◽  
Author(s):  
Motukuri S. R. Krishna ◽  
Nerella Dheeraj ◽  
Bathuru Jayasree ◽  
Chodisetty Bhavya ◽  
Nallamothu Jaswanthi
Keyword(s):  
Gene Family ◽  
In Silico ◽  
Foxtail Millet ◽  
Chitinase Gene ◽  
Setaria Italica ◽  
Genome Wide ◽  
In Silico Characterization
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