Gliosarcoma Stem Cells: Glial and Mesenchymal Differentiation

2011 ◽  
pp. 75-81
Author(s):  
Ana C. deCarvalho ◽  
Tom Mikkelsen
Keyword(s):  
Stem Cells ◽  
Mesenchymal Differentiation

Mesenchymal stem cells in infantile haemangioma

2011 ◽  
Vol 64 (3) ◽  
pp. 232-236 ◽  
Author(s):  
Tinte Itinteang ◽  
Anasuya Vishvanath ◽  
Darren J Day ◽  
Swee T Tan
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Neural Crest ◽  
Capillary Endothelium ◽  
Fatty Tissue ◽  
Rt Pcr ◽  
Functional Potential ◽  
Infantile Haemangioma ◽  
Mesenchymal Differentiation ◽  
Associated Proteins

BackgroundFibro-fatty deposition commonly occurs during involution of infantile haemangioma (IH). Mesenchymal stem cells have been identified in this tumour and have been proposed to be recruited from the bone marrow and/or adjacent niches, and then give rise to the fibro-fatty tissue. The authors have recently demonstrated that the capillary endothelium of proliferating IH co-expresses primitive mesodermal, mesenchymal and neural crest markers and proposed that this same endothelium has the ability to give rise to cells of mesenchymal lineage that constitute the fibro-fatty deposition.MethodsImmunohistochemistry and real-time RT-PCR were used to further characterise proliferating IHs and haemangioma explant-derived cells (HaemEDCs).ResultsThe authors have further confirmed expression of the mesenchymal-associated proteins including preadipocyte factor-1, a mesenchymal differentiation inhibition-associated cytokine. The HaemEDCs could be differentiated into osteoblasts and adipocytes, indicating their functional potential for terminal differentiation.DiscussionThe collective expression of neural crest, mesenchymal and mesenchymal differentiation inhibition-associated proteins on the endothelium of proliferating IH suggests that the cells in the capillary endothelium within the lesion possess the ability to undergo terminal mesenchymal differentiation during the proliferating phase, but are inhibited from doing so.

Characterization and Neural Differentiation of Fetal Lung Mesenchymal Stem Cells.

Blood ◽  
2004 ◽  
Vol 104 (11) ◽  
pp. 4177-4177
Author(s):  
Zhong Chao Han ◽  
Cun Gang ◽  
Feng Wu ◽  
Qing Jun ◽  
Shi Hong ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Flow Cytometric Analysis ◽  
Fetal Lung ◽  
Neural Cells ◽  
Alternative Source ◽  
Differentiation Potential ◽  
Cell Based Therapy ◽  
Mesenchymal Differentiation ◽  
M Phase

Abstract Mesenchymal stem cells (MSCs) have been successfully isolated from a broad range of adult, fetal and other non-embryonic tissues. Fetal lung has been identified as a rich source of MSCs capable of differentiating into multilineage cells of mesenchymal origin. However, the biological characteristics and differentiation potential of fetal lung MSCs remain to be explored. In this study, we have established a series of methods for isolation and expansion of fetal lung MSCs. These MSCs could withstand 40 passages without obvious decline in proliferation ability, significant changes in morphology and expression of cell markers. Cell cycle analysis revealed that when the MSCs reached their log phase of growth, more than 90% of the cells were in G0-G1 phase while the proportion of cells in S phase and G2-M phase were about 5.56% and 2.08% cells individually. Flow cytometric analysis showed that fetal lung MSCs expressed CD13, CD29, CD44, CD90, CD105, D117, CD166 and HLA-ABC, but not CD14, CD31, CD34, CD38, CD41a, CD42b, CD45, CD49d, CD61, CD106, CD133 and HLA-DR. These MSCs could differentiate into neural cells in addition to their mesenchymal differentiation potential. Our data suggest that the fetal lung MSC population is an alternative source of stem cells for cell-based therapy of neurological defects or mesenchymal originated diseases.

scholarly journals Nanotopographical Cues Augment Mesenchymal Differentiation of Human Embryonic Stem Cells

Small ◽  
2013 ◽  
Vol 9 (12) ◽  
pp. 2140-2151 ◽  
Author(s):  
Emmajayne Kingham ◽  
Kate White ◽  
Nikolaj Gadegaard ◽  
Matthew J. Dalby ◽  
Richard O. C. Oreffo
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Human Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Mesenchymal Differentiation

scholarly journals Biological characteristics of stem cells derived from burned skin—a comparative study with umbilical cord stem cells

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Reinhard Dolp ◽  
Gertraud Eylert ◽  
Christopher Auger ◽  
Ayesha Aijaz ◽  
Yufei Andy Chen ◽  
...  
Keyword(s):  
Stem Cells ◽  
Umbilical Cord ◽  
Mitochondrial Function ◽  
Population Doubling ◽  
Population Doubling Time ◽  
Cell Secretion ◽  
Lineage Differentiation ◽  
Burned Skin ◽  
Mesenchymal Differentiation ◽  
Stromal Stem Cells

Abstract Introduction Burned human skin, which is routinely excised and discarded, contains viable mesenchymal stromal/stem cells (burn-derived mesenchymal stromal/stem cells; BD-MSCs). These cells show promising potential to enable and aid wound regeneration. However, little is known about their cell characteristics and biological function. Objectives This study had two aims: first, to assess critical and cellular characteristics of BD-MSCs and, second, to compare those results with multipotent well-characterized MSCs from Wharton’s jelly of human umbilical cords (umbilical cord mesenchymal stromal/stem cells, UC-MSCs). Methods BD- and UC-MSCs were compared using immunophenotyping, multi-lineage differentiation, seahorse analysis for glycolytic and mitochondrial function, immune surface markers, and cell secretion profile assays. Results When compared to UC-MSCs, BD-MSCs demonstrated a lower mesenchymal differentiation capacity and altered inflammatory cytokine secretomes at baseline and after stimulation with lipopolysaccharides. No significant differences were found in population doubling time, colony formation, cell proliferation cell cycle, production of reactive oxygen species, glycolytic and mitochondrial function, and in the expression of major histocompatibility complex I and II and toll-like receptor (TLR). Importance, translation This study reveals valuable insights about MSCs obtained from burned skin and show comparable cellular characteristics with UC-MSCs, highlighting their potentials in cell therapy and skin regeneration.

scholarly journals Mesenchymal differentiation of glioblastoma stem cells

2008 ◽  
Vol 15 (9) ◽  
pp. 1491-1498 ◽  
Author(s):  
L Ricci-Vitiani ◽  
R Pallini ◽  
L M Larocca ◽  
D G Lombardi ◽  
M Signore ◽  
...  
Keyword(s):  
Stem Cells ◽  
Glioblastoma Stem Cells ◽  
Mesenchymal Differentiation

scholarly journals Bone Cells Differentiation: How CFTR Mutations May Rule the Game of Stem Cells Commitment?

2021 ◽  
Vol 9 ◽  
Author(s):  
Claire Dumortier ◽  
Soula Danopoulos ◽  
Frédéric Velard ◽  
Denise Al Alam
Keyword(s):  
Cystic Fibrosis ◽  
Stem Cells ◽  
Stem Cell ◽  
Cell Differentiation ◽  
Bone Formation ◽  
Bone Cells ◽  
Stem Cell Differentiation ◽  
Direct Role ◽  
Mesenchymal Differentiation ◽  
Cftr Mutations

Cystic fibrosis (CF)-related bone disease has emerged as a significant comorbidity of CF and is characterized by decreased bone formation and increased bone resorption. Both osteoblast and osteoclast differentiations are impacted by cystic fibrosis transmembrane conductance regulator (CFTR) mutations. The defect of CFTR chloride channel or the loss of CFTR’s ability to interact with other proteins affect several signaling pathways involved in stem cell differentiation and the commitment of these cells toward bone lineages. Specifically, TGF-β, nuclear factor-kappa B (NF-κB), PI3K/AKT, and MAPK/ERK signaling are disturbed by CFTR mutations, thus perturbing stem cell differentiation. High inflammation in patients changes myeloid lineage secretion, affecting both myeloid and mesenchymal differentiation. In osteoblast, Wnt signaling is impacted, resulting in consequences for both bone formation and resorption. Finally, CFTR could also have a direct role in osteoclast’s resorptive function. In this review, we summarize the existing literature on the role of CFTR mutations on the commitment of induced pluripotent stem cells to bone cells.

Characterization and Neural Differentiation of Fetal Lung Mesenchymal Stem Cells

2005 ◽  
Vol 14 (5) ◽  
pp. 311-321 ◽  
Author(s):  
Cun Gang Fan ◽  
Feng Wu Tang ◽  
Qing Jun Zhang ◽  
Shi Hong Lu ◽  
Hai Ying Liu ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Flow Cytometric Analysis ◽  
Fetal Lung ◽  
Neural Cells ◽  
Alternative Source ◽  
Differentiation Potential ◽  
Cell Based Therapy ◽  
Mesenchymal Differentiation ◽  
M Phase

Mesenchymal stem cells (MSCs) have been successfully isolated from a broad range of adult, fetal, and other nonembryonic tissues. Fetal lung has been identified as a rich source of MSCs. However, the biological characteristics and differentiation potential of fetal lung MSCs remain to be explored. In this study, we established a series of methods for isolation and expansion of fetal lung MSCs. These MSCs could withstand more than 40 passages without obvious decline in proliferation ability, significant changes in morphology, and expression of cell markers. Flow cytometric analysis showed that fetal lung MSCs expressed CD13, CD29, CD44, CD90, CD105, CD166, and HLA-ABC, but not CD14, CD31, CD34, CD38, CD41a, CD42b, CD45, CD49d, CD61, CD106, CD133, and HLA-DR. Cell cycle analysis revealed that when the MSCs reached their log phase of growth, more than 90% of the cells were in G0/G1 phase while the proportion of cells in S phase and G2/M phase were about 5.56% and 2.08% cells, respectively. These MSCs could differentiate into neural cells in addition to their mesenchymal differentiation potential. Our data suggest that the fetal lung MSC population is an alternative source of stem cells for cell-based therapy of neurological defects or mesenchymal-originating diseases.

Carnitine and acetylcarnitine modulate mesenchymal differentiation of adult stem cells

2013 ◽  
Vol 9 (12) ◽  
pp. 1352-1362 ◽  
Author(s):  
Qiaozhi Lu ◽  
Yuanfan Zhang ◽  
Jennifer H. Elisseeff
Keyword(s):  
Stem Cells ◽  
Adult Stem Cells ◽  
Mesenchymal Differentiation

scholarly journals Phosphoinositide 3 Kinase Signaling in Human Stem Cells from Reprogramming to Differentiation: A Tale in Cytoplasmic and Nuclear Compartments

2019 ◽  
Vol 20 (8) ◽  
pp. 2026 ◽  
Author(s):  
Ramazzotti ◽  
Ratti ◽  
Fiume ◽  
Yung Follo ◽  
Billi ◽  
...  
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Therapeutic Potential ◽  
Induced Pluripotent Stem Cell ◽  
Cellular Transformation ◽  
Cell Types ◽  
Human Stem Cells ◽  
Mesenchymal Differentiation ◽  
Induced Pluripotent ◽  
Phosphoinositide 3 Kinase

Stem cells are undifferentiated cells that can give rise to several different cell types and can self-renew. Given their ability to differentiate into different lineages, stem cells retain huge therapeutic potential for regenerative medicine. Therefore, the understanding of the signaling pathways involved in stem cell pluripotency maintenance and differentiation has a paramount importance in order to understand these biological processes and to develop therapeutic strategies. In this review, we focus on phosphoinositide 3 kinase (PI3K) since its signaling pathway regulates many cellular processes, such as cell growth, proliferation, survival, and cellular transformation. Precisely, in human stem cells, the PI3K cascade is involved in different processes from pluripotency and induced pluripotent stem cell (iPSC) reprogramming to mesenchymal and oral mesenchymal differentiation, through different and interconnected mechanisms.

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