Sphingomyelin and Its Role in Cellular Signaling

2013 ◽  
pp. 1-14 ◽  
Author(s):  
Mahua Chakraborty ◽  
Xian-Cheng Jiang
Keyword(s):  
Cellular Signaling

scholarly journals Intracellular Ionic Strength Sensing Using NanoLuc

2021 ◽  
Vol 22 (2) ◽  
pp. 677
Author(s):  
Tausif Altamash ◽  
Wesam Ahmed ◽  
Saad Rasool ◽  
Kabir H. Biswas
Keyword(s):  
Thermal Stability ◽  
Phase Separation ◽  
Drug Discovery ◽  
Ionic Strength ◽  
Cellular Signaling ◽  
Live Cells ◽  
Protein Activity ◽  
Cellular Survival ◽  
Cellular Processes ◽  
Wide Range

Intracellular ionic strength regulates myriad cellular processes that are fundamental to cellular survival and proliferation, including protein activity, aggregation, phase separation, and cell volume. It could be altered by changes in the activity of cellular signaling pathways, such as those that impact the activity of membrane-localized ion channels or by alterations in the microenvironmental osmolarity. Therefore, there is a demand for the development of sensitive tools for real-time monitoring of intracellular ionic strength. Here, we developed a bioluminescence-based intracellular ionic strength sensing strategy using the Nano Luciferase (NanoLuc) protein that has gained tremendous utility due to its high, long-lived bioluminescence output and thermal stability. Biochemical experiments using a recombinantly purified protein showed that NanoLuc bioluminescence is dependent on the ionic strength of the reaction buffer for a wide range of ionic strength conditions. Importantly, the decrease in the NanoLuc activity observed at higher ionic strengths could be reversed by decreasing the ionic strength of the reaction, thus making it suitable for sensing intracellular ionic strength alterations. Finally, we used an mNeonGreen–NanoLuc fusion protein to successfully monitor ionic strength alterations in a ratiometric manner through independent fluorescence and bioluminescence measurements in cell lysates and live cells. We envisage that the biosensing strategy developed here for detecting alterations in intracellular ionic strength will be applicable in a wide range of experiments, including high throughput cellular signaling, ion channel functional genomics, and drug discovery.

scholarly journals The Roles of Mitochondrial Reactive Oxygen Species in Cellular Signaling and Stress Response in Plants

2016 ◽  
Vol 171 (3) ◽  
pp. 1551-1559 ◽  
Author(s):  
Shaobai Huang ◽  
Olivier Van Aken ◽  
Markus Schwarzländer ◽  
Katharina Belt ◽  
A. Harvey Millar
Keyword(s):  
Reactive Oxygen Species ◽  
Stress Response ◽  
Cellular Signaling ◽  
Reactive Oxygen ◽  
Oxygen Species ◽  
Mitochondrial Reactive Oxygen Species

Oncogenic Activities of IDH1/2 Mutations: From Epigenetics to Cellular Signaling

2017 ◽  
Vol 27 (10) ◽  
pp. 738-752 ◽  
Author(s):  
Laurence M. Gagné ◽  
Karine Boulay ◽  
Ivan Topisirovic ◽  
Marc-Étienne Huot ◽  
Frédérick A. Mallette
Keyword(s):  
Cellular Signaling
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