Stochastic Approach for Enzyme Reaction in Nano Size via Different Algorithms

2013 ◽  
pp. 189-206
Author(s):  
Farid Taherkhani ◽  
Shahram Ranjbar
Keyword(s):  
Enzyme Reaction ◽  
Stochastic Approach ◽  
Nano Size

Lead aspartate: a new en bloc stain for electron microscopy

1978 ◽  
Vol 36 (2) ◽  
pp. 34-35
Author(s):  
J.R. Walton
Keyword(s):  
Electron Microscopy ◽  
Calcium Ion ◽  
Enzyme Reaction ◽  
Lead Citrate ◽  
Reaction Products ◽  
En Bloc ◽  
Thin Sections ◽  
Lead Salts ◽  
Thin Sectioning ◽  
High Alkalinity

In electron microscopy, lead is the metal most widely used for enhancing specimen contrast. Lead citrate requires a pH of 12 to stain thin sections of epoxy-embedded material rapidly and intensively. However, this high alkalinity tends to leach out enzyme reaction products, making lead citrate unsuitable for many cytochemical studies. Substitution of the chelator aspartate for citrate allows staining to be carried out at pH 6 or 7 without apparent effect on cytochemical products. Moreover, due to the low, controlled level of free lead ions, contamination-free staining can be carried out en bloc, prior to dehydration and embedding. En bloc use of lead aspartate permits the grid-staining step to be bypassed, allowing samples to be examined immediately after thin-sectioning.Procedures. To prevent precipitation of lead salts, double- or glass-distilled H20 used in the stain and rinses should be boiled to drive off carbon dioxide and glassware should be carefully rinsed to remove any persisting traces of calcium ion.

TEM investigations of surface residual stress relaxation in A12O3 and Al2O3-SiC nanocomposite

1994 ◽  
Vol 52 ◽  
pp. 628-629
Author(s):  
J. Fang ◽  
H. M. Chan ◽  
M. P. Harmer
Keyword(s):  
Residual Stress ◽  
Single Phase ◽  
Stress Relief ◽  
Stress Recovery ◽  
Surface Residual Stress ◽  
Microscopic Mechanism ◽  
Sic Particles ◽  
Annealing Procedure ◽  
The Difference ◽  
Nano Size

It was Niihara et al. who first discovered that the fracture strength of Al2O3 can be increased by incorporating as little as 5 vol.% of nano-size SiC particles (>1000 MPa), and that the strength would be improved further by a simple annealing procedure (>1500 MPa). This discovery has stimulated intense interest on Al2O3/SiC nanocomposites. Recent indentation studies by Fang et al. have shown that residual stress relief was more difficult in the nanocomposite than in pure Al2O3. In the present work, TEM was employed to investigate the microscopic mechanism(s) for the difference in the residual stress recovery in these two materials.Bulk samples of hot-pressed single phase Al2O3, and Al2O3 containing 5 vol.% 0.15 μm SiC particles were simultaneously polished with 15 μm diamond compound. Each sample was cut into two pieces, one of which was subsequently annealed at 1300° for 2 hours in flowing argon. Disks of 3 mm in diameter were cut from bulk samples.

Computer Simulation of the Response of Amperometric Biosensors in Stirred and non Stirred Solution

2003 ◽  
Vol 8 (1) ◽  
pp. 3-18 ◽  
Author(s):  
R. Baronas ◽  
F. Ivanauskas ◽  
J. Kulys
Keyword(s):  
Mathematical Model ◽  
Computer Simulation ◽  
Finite Difference ◽  
Mass Transport ◽  
Enzyme Reaction ◽  
Analyte Concentration ◽  
Amperometric Biosensors ◽  
Finite Difference Technique ◽  
Enzyme Layer ◽  
Biosensor Response

A mathematical model of amperometric biosensors has been developed to simulate the biosensor response in stirred as well as non stirred solution. The model involves three regions: the enzyme layer where enzyme reaction as well as mass transport by diffusion takes place, a diffusion limiting region where only the diffusion takes place, and a convective region, where the analyte concentration is maintained constant. Using computer simulation the influence of the thickness of the enzyme layer as well the diffusion one on the biosensor response was investigated. The computer simulation was carried out using the finite difference technique.

LIMITATIONS OF THE STOCHASTIC APPROACH IN TWO-PHASE TURBULENT FLOW CALCULATIONS

1996 ◽  
Vol 6 (2) ◽  
pp. 211-225 ◽  
Author(s):  
Keh-Chin Chang ◽  
Wen-Jing Wu ◽  
Muh-Rong Wang
Keyword(s):  
Turbulent Flow ◽  
Stochastic Approach ◽  
Two Phase

STOCHASTIC CALCULATION OF LIFETIME PREDICTION FOR COMPONENTS IN METAL STRUCTURES DUE TO THE GROWTH OF MICRODEFECTS

2020 ◽  
Vol 4 (97) ◽  
pp. 69-76
Author(s):  
IGOR N. SILVERSTOV
Keyword(s):  
Fatigue Strength ◽  
Fracture Mechanics ◽  
Stochastic Approach ◽  
Fatigue Curve ◽  
Probability Of Failure ◽  
Lifetime Prediction ◽  
Statistical Distributions ◽  
Metal Structures

A stochastic approach has been developed to evaluate fatigue strength using elements of the fracture mechanics. The article presents a method for determining the initial parameters of statistical distributions. It also considers the method for constructing a fatigue curve for a component of any size and configuration with any given probability of failure.

Machine Learning for Acute Toxicity Prediction Using High-Throughput Enzyme-Reaction Chip

2019 ◽  
Author(s):  
Qiannan Duan ◽  
Jianchao Lee ◽  
Jinhong Gao ◽  
Jiayuan Chen ◽  
Yachao Lian ◽  
...  
Keyword(s):  
Machine Learning ◽  
Acute Toxicity ◽  
Model Building ◽  
Low Cost ◽  
Enzyme Reaction ◽  
Chemical Effect ◽  
Technological Innovations ◽  
Acute Toxicity Test ◽  
Toxicity Prediction ◽  
Traditional Understanding

<p>Machine learning (ML) has brought significant technological innovations in many fields, but it has not been widely embraced by most researchers of natural sciences to date. Traditional understanding and promotion of chemical analysis cannot meet the definition and requirement of big data for running of ML. Over the years, we focused on building a more versatile and low-cost approach to the acquisition of copious amounts of data containing in a chemical reaction. The generated data meet exclusively the thirst of ML when swimming in the vast space of chemical effect. As proof in this study, we carried out a case for acute toxicity test throughout the whole routine, from model building, chip preparation, data collection, and ML training. Such a strategy will probably play an important role in connecting ML with much research in natural science in the future.</p>

Investigation of the effect of loratadine and calcium ions on oxidoreductase activity of catalase enzyme

2020 ◽  
Vol 16 ◽  
Author(s):  
Edhem Hasković ◽  
Safija Herenda ◽  
Zehra Halilović ◽  
Snežana Unčanin ◽  
Denis Hasković ◽  
...  
Keyword(s):  
Calcium Ions ◽  
Enzyme Reaction ◽  
Analytical Techniques ◽  
Competitive Inhibitor ◽  
Cellular Functions ◽  
Oxidoreductase Activity ◽  
H1 Antihistamines ◽  
Activity Of Enzymes ◽  
The Given

Background: The Spectrophotometric method is one of the most suitable analytical techniques for testing the activity of enzymes under the influence of various factors. Methods: The effect of H1-antihistamines of loratadine and calcium ions on enzyme catalase under in vitro conditions was investigated in this paper. Results and Discussion: It has been shown that loratadine isa partial inhibitor of catalase, but this effect is diminished in the presence of calcium ions. Calcium as well as other cations are important for many biological and cellular functions. The kidneys play a central role in the homeostasis of these ions. The activity of the catalase enzyme under the given conditions, the type of inhibition,and the kinetic parameters of the enzyme reaction were determined. Conclusion: We concluded that loratadine is a partially competitive inhibitor.

Sign in / Sign up

Export Citation Format

Share Document