A stochastic approach to a one substrate, one product enzyme reaction in the initial velocity phase

1969 ◽  
Vol 25 (1) ◽  
pp. 159-172 ◽  
Author(s):  
C.C. Heyde ◽  
Elizabeth Heyde
Keyword(s):  
Initial Velocity ◽  
Enzyme Reaction ◽  
Stochastic Approach

scholarly journals An approach to better estimation of the intial velocity of enzyme reactions

2019 ◽  
Vol 488 (2) ◽  
pp. 225-228
Author(s):  
N. M. Malygina ◽  
T. A. Petrova ◽  
A. Y. Lianguzov ◽  
A. M. Ivanov
Keyword(s):  
Initial Velocity ◽  
Kinetic Curve ◽  
Enzyme Reaction ◽  
Lag Phase ◽  
Activity Levels ◽  
Salivary Alpha Amylase ◽  
Reaction Velocity ◽  
Enzyme Reactions ◽  
Enzyme Systems ◽  
Assay Time

A new algorithm for computation of initial velocity of enzyme reaction at the time zero is proposed. This algorithm makes it possible to reduce systematic error of measurements to the minimum, to estimate reaction velocity in testing samples regardless of the enzyme activity levels and to minimize assay time. The study is illustrated by an example of salivary alpha-amylase and standard reagent kit. The algorithm should not be applied if conjugated enzyme systems are used because there is a long initial lag-phase in the kinetic curve.

scholarly journals Adenosine 5′-triphosphate sulphurylase from Saccharomyces cerevisiae

1973 ◽  
Vol 133 (3) ◽  
pp. 541-550 ◽  
Author(s):  
Catherine S. Hawes ◽  
D. J. D. Nicholas
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Initial Velocity ◽  
Isotopic Exchange ◽  
Competitive Inhibition ◽  
Gel Filtration ◽  
Deae Cellulose ◽  
Enzyme Reaction ◽  
Velocity Data ◽  
Sequential Reaction ◽  
Optimum Reaction

1. ATP sulphurylase from Saccharomyces cerevisiae was purified 140-fold by using heat treatment, DEAE-cellulose chromatography and Sepharose 6B gel filtration. 2. The enzyme was stable at -15°C, optimum reaction velocity was between pH7.0 and 9.0, and the activation energy was 62kJ/mol (14.7kcal/mol). 3. The substrate was shown to be the MgATP2- complex, free ATP being inhibitory. 4. Double-reciprocal plots from initial-velocity studies were intersecting and the Km of each substrate was determined at infinite concentration of the other (Km MgATP2-, 0.07mm; MoO42-, 0.17mm). 5. Radio-isotopic exchange between the substrate pairs, adenosine 5′-[35S]sulphatophosphate and SO42-, 35SO42- and adenosine 5′-sulphatophosphate, occurred only in the presence of either MgATP2- or PPi. This suggests, along with the initial-velocity data, a sequential reaction mechanism in which both substrates bind before any product is released. 6. The enzyme reaction was specific for ATP and was not inhibited by l-cysteine, l-methionine, SO32-, S2O32- (all 2mm) nor by p-chloromercuribenzoate (1mm). 7. Competitive inhibition of the enzyme with respect to MoO42- was produced by SO42- (Ki=2.0mm) and non-competitive inhibition by sulphide (Ki=3.4mm). 8. Adenosine 5′-sulphatophosphate inhibited strongly and concentrations as low as 0.02mm altered the normal hyperbolic velocity–substrate curves with both MgATP2- and MoO42- to sigmoidal forms.

Kinetic studies of sulfite; cytochrome c oxidoreductase, thiosulfate-oxidizing enzyme, and adenostne-5′-phosphosulfate reductase from Thiobacillus thioparus

1970 ◽  
Vol 48 (5) ◽  
pp. 594-603 ◽  
Author(s):  
Ronald M. Lyric ◽  
Isamu Suzuki
Keyword(s):  
Cytochrome C ◽  
Initial Velocity ◽  
Enzyme Reaction ◽  
Kinetic Studies ◽  
Kinetic Mechanism ◽  
Product Inhibition ◽  
Thiobacillus Thioparus ◽  
Inhibition Studies

Kinetic studies were carried out on three enzymes purified from Thiobacillus thioparus: sulfite: cytochrome c oxidoreductase, thiosulfate-oxidizing enzyme, and adenosine-5′-phosphosulfate reductase. From the initial velocity and product inhibition studies a tentative kinetic mechanism was proposed for each enzyme reaction.

Lead aspartate: a new en bloc stain for electron microscopy

1978 ◽  
Vol 36 (2) ◽  
pp. 34-35
Author(s):  
J.R. Walton
Keyword(s):  
Electron Microscopy ◽  
Calcium Ion ◽  
Enzyme Reaction ◽  
Lead Citrate ◽  
Reaction Products ◽  
En Bloc ◽  
Thin Sections ◽  
Lead Salts ◽  
Thin Sectioning ◽  
High Alkalinity

In electron microscopy, lead is the metal most widely used for enhancing specimen contrast. Lead citrate requires a pH of 12 to stain thin sections of epoxy-embedded material rapidly and intensively. However, this high alkalinity tends to leach out enzyme reaction products, making lead citrate unsuitable for many cytochemical studies. Substitution of the chelator aspartate for citrate allows staining to be carried out at pH 6 or 7 without apparent effect on cytochemical products. Moreover, due to the low, controlled level of free lead ions, contamination-free staining can be carried out en bloc, prior to dehydration and embedding. En bloc use of lead aspartate permits the grid-staining step to be bypassed, allowing samples to be examined immediately after thin-sectioning.Procedures. To prevent precipitation of lead salts, double- or glass-distilled H20 used in the stain and rinses should be boiled to drive off carbon dioxide and glassware should be carefully rinsed to remove any persisting traces of calcium ion.

Computer Simulation of the Response of Amperometric Biosensors in Stirred and non Stirred Solution

2003 ◽  
Vol 8 (1) ◽  
pp. 3-18 ◽  
Author(s):  
R. Baronas ◽  
F. Ivanauskas ◽  
J. Kulys
Keyword(s):  
Mathematical Model ◽  
Computer Simulation ◽  
Finite Difference ◽  
Mass Transport ◽  
Enzyme Reaction ◽  
Analyte Concentration ◽  
Amperometric Biosensors ◽  
Finite Difference Technique ◽  
Enzyme Layer ◽  
Biosensor Response

A mathematical model of amperometric biosensors has been developed to simulate the biosensor response in stirred as well as non stirred solution. The model involves three regions: the enzyme layer where enzyme reaction as well as mass transport by diffusion takes place, a diffusion limiting region where only the diffusion takes place, and a convective region, where the analyte concentration is maintained constant. Using computer simulation the influence of the thickness of the enzyme layer as well the diffusion one on the biosensor response was investigated. The computer simulation was carried out using the finite difference technique.

LIMITATIONS OF THE STOCHASTIC APPROACH IN TWO-PHASE TURBULENT FLOW CALCULATIONS

1996 ◽  
Vol 6 (2) ◽  
pp. 211-225 ◽  
Author(s):  
Keh-Chin Chang ◽  
Wen-Jing Wu ◽  
Muh-Rong Wang
Keyword(s):  
Turbulent Flow ◽  
Stochastic Approach ◽  
Two Phase

STOCHASTIC CALCULATION OF LIFETIME PREDICTION FOR COMPONENTS IN METAL STRUCTURES DUE TO THE GROWTH OF MICRODEFECTS

2020 ◽  
Vol 4 (97) ◽  
pp. 69-76
Author(s):  
IGOR N. SILVERSTOV
Keyword(s):  
Fatigue Strength ◽  
Fracture Mechanics ◽  
Stochastic Approach ◽  
Fatigue Curve ◽  
Probability Of Failure ◽  
Lifetime Prediction ◽  
Statistical Distributions ◽  
Metal Structures

A stochastic approach has been developed to evaluate fatigue strength using elements of the fracture mechanics. The article presents a method for determining the initial parameters of statistical distributions. It also considers the method for constructing a fatigue curve for a component of any size and configuration with any given probability of failure.

Machine Learning for Acute Toxicity Prediction Using High-Throughput Enzyme-Reaction Chip

2019 ◽  
Author(s):  
Qiannan Duan ◽  
Jianchao Lee ◽  
Jinhong Gao ◽  
Jiayuan Chen ◽  
Yachao Lian ◽  
...  
Keyword(s):  
Machine Learning ◽  
Acute Toxicity ◽  
Model Building ◽  
Low Cost ◽  
Enzyme Reaction ◽  
Chemical Effect ◽  
Technological Innovations ◽  
Acute Toxicity Test ◽  
Toxicity Prediction ◽  
Traditional Understanding

<p>Machine learning (ML) has brought significant technological innovations in many fields, but it has not been widely embraced by most researchers of natural sciences to date. Traditional understanding and promotion of chemical analysis cannot meet the definition and requirement of big data for running of ML. Over the years, we focused on building a more versatile and low-cost approach to the acquisition of copious amounts of data containing in a chemical reaction. The generated data meet exclusively the thirst of ML when swimming in the vast space of chemical effect. As proof in this study, we carried out a case for acute toxicity test throughout the whole routine, from model building, chip preparation, data collection, and ML training. Such a strategy will probably play an important role in connecting ML with much research in natural science in the future.</p>

APPLICATION OF THE METHOD SPRINGY DEFORMATIONS OF BARREL DURING SHOT FOR DETERMINING THE INITIAL VELOCITY OF THE SHELL (MINE)

2019 ◽  
Vol 1 (12) ◽  
pp. 75-80
Author(s):  
Ye. Didenko ◽  
O. Stepanenko
Keyword(s):  
Strain Gauge ◽  
Initial Velocity ◽  
Radial Direction ◽  
Fire Control ◽  
Automatic Control Systems ◽  
Measurement Results ◽  
Initial Resistance ◽  
The Moment ◽  
The Many ◽  
Effective Use

One of the indicators of the effective use of artillery is the accuracy of the fire impact on the objects of enemy. The accuracy of the artillery is achieved by completing the implementation of all measures for the preparation of shooting and fire control. Main measures of ballistic preparation are to determine and take into account the summary deviation of the initial velocity. The existing procedure for determining the summary deviation of the initial velocity for the check (main) cannon of battery leads to accumulation of ballistic preparation errors. The supply of artillery units with means of determining the initial speed of the projectile is insufficient. Among the many known methods for measuring the initial velocity, not enough attention was paid to the methods of analyzing the processes that occur during a shot in the "charge-shell-barrel" system. Under the action of the pressure of the powder gases in the barrel channel and the forces of the interaction of the projectile with the barrel there are springy deformations in the radial direction. To measure springy deformations it is advisable to use strain gauge sensors. Monitoring of deformation in a radial direction by time can be used to determine the moment of passing a projectile past the strain gauge mounted on the outer surface of the barrel. In the case of springy deformations, the initial resistance of the sensor varies in proportion to its value. The speed of the shell (mine) in the barrel can be determined by time between pulses of signals obtained from strain gauges located at a known distance from each other. The simplicity of the proposed method for measuring the initial velocity of an artillery shell provides an opportunity for equipping each cannon (mortar) with autonomous means for measuring the initial velocity. With the simultaneous puting into action of automatic control systems can be automatically taking into account the measurement results. This will change the existing procedure for determining the total deviation of the initial velocity and improve the accuracy, timeliness and suddenness of the opening of artillery fire, which are components of its efficiency.

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