Human Peyer’s patches and intestinal lamina propria contain remarkably different numbers of dendritic cells positive for S-100 protein and macrophages positive for L1 antigen

1990 ◽  
pp. 617-618
Author(s):  
K Bjerke ◽  
P Brandtzaeg
Keyword(s):  
Dendritic Cells ◽  
Lamina Propria ◽  
Peyer's Patches ◽  
Peyer’S Patches ◽  
Intestinal Lamina Propria ◽  
S 100

scholarly journals P073 Compartmentalised human gut immunity: studies of innate and adaptive lymphocyte distribution in the epithelium, lamina propria and GALT of healthy gut mucosa by flow cytometry

2020 ◽  
Vol 14 (Supplement_1) ◽  
pp. S172-S172
Author(s):  
A Carrasco Garcia ◽  
A Rao ◽  
E Kokkinou ◽  
S Haapaniemi ◽  
U Lindforss ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
T Cell ◽  
Lamina Propria ◽  
Immune Cell ◽  
Cd8 T Cell ◽  
Peyer's Patches ◽  
Peyer’S Patches ◽  
Human Gut ◽  
Cell Ratio ◽  
Health And Disease

Abstract Background The human gut mucosal immune system is compartmentalised in distinct and specialised immune niches. The epithelium and the lamina propria have been proposed as effector sites, while gut-associated lymphoid tissues (GALTs) constitute inductive immune niches. The major mucosal GALTs are the Peyer’s patches in the ileum and the colonic isolated lymphoid follicles (ILFs), scattered in the submucosa of the colon. The majority of studies of human gut immune function in health and disease have analysed unfractionated mucosal tissue samples. Hence, in contrast to mice, little is known about compartmentalised immune cell specialisation in the human gut. The aim of this study was to use novel dissection methods to analyse separate human gut immune niches. Methods Macroscopically healthy margins from colorectal cancer colectomies were obtained at a minimum distance of 10 cm from the tumour border. After faeces, mucus, fat and muscle removal, Peyer’s patches were identified and dissected using a stereomicroscope (based on Keita et al., Lab Invest, 2006). Colonic mucosa and submucosa (containing ILFs) fractions were mechanically separated by forceps (based on the method developed by Fenton et al., Immunity, under revision). Isolation of epithelial and lamina propria fractions from the mucosal compartment was performed by calcium chelation (DTT and EDTA) and enzymatic digestion (Collagenase II and DNAse), respectively. Cell suspensions from each fraction were analysed by flow cytometry (BD LSR-Fortessa and BD FACSymphony). Results As expected, mucosal GALTs were characterised by an enrichment of germinal centre B cells (CD19+CD20+CD38+), lymphoid tissue-like innate lymphoid cells (Lin−CD127+CD117+Nrp1+) and a higher CD4+/CD8+ T-cell ratio vs. mucosa, whereas the mucosal fraction was enriched for plasma cells (CD19+CD20−CD38high) and distinguished by a decreased CD4+/CD8+ T-cell ratio as compared with the GALT in both ileum and colon. CD19+/CD3+ ratios were only higher in Peyer’s patches but not in colonic submucosa enriched with ILFs, possibly due to the smaller size of the B-cell follicles in the latter. The intraepithelial compartment lacked B cells and contained more γδ-T cells as compared with the GALT and lamina propria. Conclusion We have used novel dissection methods in human intestinal tissues that reveal a compartmentalised immune cell specialisation that is in line with what has previously been described in mice. The method will allow for future deeper analysis of the human gut immune niches in health and disease, such as in inflammatory bowel disease.

scholarly journals Intestinal lamina propria dendritic cells maintain T cell homeostasis but do not affect commensalism

2013 ◽  
Vol 210 (10) ◽  
pp. 2011-2024 ◽  
Author(s):  
Nathan E. Welty ◽  
Christopher Staley ◽  
Nico Ghilardi ◽  
Michael J. Sadowsky ◽  
Botond Z. Igyártó ◽  
...  
Keyword(s):  
T Cells ◽  
Dendritic Cells ◽  
T Cell ◽  
Lamina Propria ◽  
Th17 Cells ◽  
Intestinal Infection ◽  
T Cell Homeostasis ◽  
Cd11b Expression ◽  
Cell Homeostasis ◽  
Intestinal Lamina Propria

Dendritic cells (DCs) in the intestinal lamina propria (LP) are composed of two CD103+ subsets that differ in CD11b expression. We report here that Langerin is expressed by human LP DCs and that transgenic human langerin drives expression in CD103+CD11b+ LP DCs in mice. This subset was ablated in huLangerin-DTA mice, resulting in reduced LP Th17 cells without affecting Th1 or T reg cells. Notably, cognate DC–T cell interactions were not required for Th17 development, as this response was intact in huLangerin-Cre I-Aβfl/fl mice. In contrast, responses to intestinal infection or flagellin administration were unaffected by the absence of CD103+CD11b+ DCs. huLangerin-DTA x BatF3−/− mice lacked both CD103+ LP DC subsets, resulting in defective gut homing and fewer LP T reg cells. Despite these defects in LP DCs and resident T cells, we did not observe alterations of intestinal microbial communities. Thus, CD103+ LP DC subsets control T cell homeostasis through both nonredundant and overlapping mechanisms.

Effect of bombesin on [Ca++]i and proliferation of lymphocytes isolated from lamina propria, peyer's patches, and spleen

1992 ◽  
Vol 40 (2) ◽  
pp. 273
Author(s):  
R Waetke ◽  
RM Liehr ◽  
K Bordasch ◽  
H Haller ◽  
M Zeitz ◽  
...  
Keyword(s):  
Lamina Propria ◽  
Peyer's Patches ◽  
Peyer’S Patches ◽  
Proliferation Of Lymphocytes

scholarly journals Oral Prion Disease Pathogenesis Is Impeded in the Specific Absence of CXCR5-Expressing Dendritic Cells

2017 ◽  
Vol 91 (10) ◽  
Author(s):  
Barry M. Bradford ◽  
Boris Reizis ◽  
Neil A. Mabbott
Keyword(s):  
Dendritic Cells ◽  
Prion Disease ◽  
Disease Susceptibility ◽  
Peyer's Patches ◽  
Oral Exposure ◽  
Peyer’S Patches ◽  
Follicular Dendritic Cells ◽  
Follicular Dendritic ◽  
Host Infection ◽  
The Brain

ABSTRACT After oral exposure, the early replication of certain prion strains upon stromal cell-derived follicular dendritic cells (FDC) in the Peyer's patches in the small intestine is essential for the efficient spread of disease to the brain. However, little is known of how prions are initially conveyed from the gut lumen to establish infection on FDC. Our previous data suggest that mononuclear phagocytes such as CD11c+ conventional dendritic cells play an important role in the initial propagation of prions from the gut lumen into Peyer's patches. However, whether these cells conveyed orally acquired prions toward FDC within Peyer's patches was not known. The chemokine CXCL13 is expressed by FDC and follicular stromal cells and modulates the homing of CXCR5-expressing cells toward the FDC-containing B cell follicles. Here, novel compound transgenic mice were created in which a CXCR5 deficiency was specifically restricted to CD11c+ cells. These mice were used to determine whether CXCR5-expressing conventional dendritic cells propagate prions toward FDC after oral exposure. Our data show that in the specific absence of CXCR5-expressing conventional dendritic cells the early accumulation of prions upon FDC in Peyer's patches and the spleen was impaired, and disease susceptibility significantly reduced. These data suggest that CXCR5-expressing conventional dendritic cells play an important role in the efficient propagation of orally administered prions toward FDC within Peyer's patches in order to establish host infection. IMPORTANCE Many natural prion diseases are acquired by oral consumption of contaminated food or pasture. Once the prions reach the brain they cause extensive neurodegeneration, which ultimately leads to death. In order for the prions to efficiently spread from the gut to the brain, they first replicate upon follicular dendritic cells within intestinal Peyer's patches. How the prions are first delivered to follicular dendritic cells to establish infection was unknown. Understanding this process is important since treatments which prevent prions from infecting follicular dendritic cells can block their spread to the brain. We created mice in which mobile conventional dendritic cells were unable to migrate toward follicular dendritic cells. In these mice the early accumulation of prions on follicular dendritic cells was impaired and oral prion disease susceptibility was reduced. This suggests that prions exploit conventional dendritic cells to facilitate their initial delivery toward follicular dendritic cells to establish host infection.

Oral scrapie infection modifies the homeostasis of Peyer’s patches’ dendritic cells

2007 ◽  
Vol 128 (3) ◽  
pp. 243-251 ◽  
Author(s):  
Gauthier Dorban ◽  
Valérie Defaweux ◽  
Etienne Levavasseur ◽  
Caroline Demonceau ◽  
Olivier Thellin ◽  
...  
Keyword(s):  
Dendritic Cells ◽  
Peyer's Patches ◽  
Peyer’S Patches ◽  
Scrapie Infection
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