Incidence of Growth Regulators on RNA/ DNA Levels in Protoplasts as Analysed by Acridine Orange Staining and Flow Cytometry

1988 ◽  
pp. 145-146
Author(s):  
C. Bergounioux ◽  
C. Perennes ◽  
S. C. Brown ◽  
C. Sarda ◽  
P. Gadal
Keyword(s):  
Flow Cytometry ◽  
Acridine Orange ◽  
Growth Regulators ◽  
Acridine Orange Staining

scholarly journals Total cellular RNA content: correlation between flow cytometry and ultraviolet spectroscopy.

1980 ◽  
Vol 28 (6) ◽  
pp. 493-498 ◽  
Author(s):  
K D Bauer ◽  
L A Dethlefsen
Keyword(s):  
Flow Cytometry ◽  
Acridine Orange ◽  
Chinese Hamster Ovary ◽  
Chinese Hamster ◽  
Ultraviolet Spectroscopy ◽  
Acridine Orange Staining ◽  
Red Fluorescence ◽  
Rna Content ◽  
Hela S3 ◽  
Hela S3 Cells

Total RNA content in Chinese hamster ovary and HeLa-S3 cells determined by ultraviolet spectroscopy is compared with the red fluorescence distribution of acridine orange-stained cells observed by flow cytometry. A correlation coefficient of 0.93 is obtained when these methods of estimating RNA content are compared after various RNAse treatments. These data suggest that acridine orange staining effectively quantitates total cellular RNA content when analyzed by flow cytometry, although DNA is also shown to contribute a low but significant background of red fluorescence.

The Study of Acute Leukemia Cells by Means of Acridine Orange Staining and Flow Cytometry

1994 ◽  
Vol 13 (1-2) ◽  
pp. 61-73 ◽  
Author(s):  
H. D. Preisler ◽  
A. Raza ◽  
V. Gopal ◽  
S. D. Banavali ◽  
J. Bokhari ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Acute Leukemia ◽  
Acridine Orange ◽  
Leukemia Cells ◽  
Acridine Orange Staining

scholarly journals A double-blinded comparison of in situ TUNEL and aniline blue versus flow cytometry acridine orange for the determination of sperm DNA fragmentation and nucleus decondensation state index

Zygote ◽  
2014 ◽  
Vol 23 (4) ◽  
pp. 556-562 ◽  
Author(s):  
Jamal Hamidi ◽  
Christophe Frainais ◽  
Edouard Amar ◽  
Eric Bailly ◽  
Patrice Clément ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Acridine Orange ◽  
Dna Fragmentation ◽  
Aniline Blue ◽  
Sperm Dna Fragmentation ◽  
Acridine Orange Staining ◽  
Sperm Dna ◽  
Double Blinded ◽  
The Impact

SummaryThe impact of sperm DNA fragmentation on assisted reproductive technology (ART) successes, in terms of outcome, is now established. High levels of DNA strand breaks severely affect the probability of pregnancy. The importance of sperm nucleus condensation in early embryogenesis and, subsequently, on the quality of the conceptus is now emerging. In this article we have compared in situ analyses with terminal deoxynucleotidyl transferase (TdT)-mediated dUTP nick end labelling (TUNEL) (for DNA fragmentation) with aniline blue (AB) (for nucleus decondensation), versus flow cytometry (FC) after acridine orange staining, in a double-blinded analysis. In our hands, TUNEL and acridine orange give perfectly comparable results. For decondensation the results are also comparable, but the double-stranded green fluorescence obtained with acridine orange seems to slightly underestimate the decondensation status obtained with AB.

STUDIES ON THE BIOLOGICAL PROPERTIES AND CLASSIFICATION OF SV4 VIRUS

1965 ◽  
Vol 11 (2) ◽  
pp. 325-335 ◽  
Author(s):  
S. A. Sattar ◽  
K. R. Rozee
Keyword(s):  
Electron Microscope ◽  
Rhesus Monkey ◽  
Red Blood Cells ◽  
Acridine Orange ◽  
Blood Cells ◽  
Magnesium Chloride ◽  
Fluorescent Microscopy ◽  
Biological Properties ◽  
Acridine Orange Staining

Cytopathic changes in LLC-MK2 cells infected with SV4 virus, observed with the electron microscope and using acridine orange staining and fluorescent microscopy, have been shown to be similar to that caused by picornaviruses and members of the Columbia-SK virus group. The virus was found to be stabilized against heat in the presence of molar magnesium chloride, and to be stable at pH 3.5. The virus was non-pathogenic for suckling mice, failed to agglutinate sheep and human "O" red blood cells, but agglutinated rhesus monkey erythrocytes at 4 °C. On the basis of these properties and those already known, it was suggested that SV4 virus be placed in the group Enteroviruses of lower animals.

scholarly journals CYTOPLASMIC DNA SYNTHESIS IN AMOEBA PROTEUS

1962 ◽  
Vol 15 (3) ◽  
pp. 535-540 ◽  
Author(s):  
M. Rabinovitch ◽  
W. Plaut
Keyword(s):  
Nucleic Acid ◽  
Dna Synthesis ◽  
Acridine Orange ◽  
Particle Number ◽  
Amoeba Proteus ◽  
Acridine Orange Staining ◽  
Particle Population ◽  
Cell Age ◽  
Cytoplasmic Dna ◽  
Number Of Particles

Nucleic acid-containing particles in the cytoplasm of Amoeba proteus (cf. reference 1) were counted after acridine orange staining. The number of particles per ameba was found to be correlated with cell age and size. Fresh daughters had a mean particle number of 5400, whereas predivision amebae contained around 11,000 particles. Amebae from two other strains contained similar particles. The particles were found to be clustered in fasted cells and redispersed after feeding. A marked increase in the particle population was noted in anucleate fragments. These results, together with those previously presented, suggest that the particles multiply intracellularly. Their nature and their relationship to previous work on nucleic acid labeling in Amoeba are discussed.

The fluorescent staining of a mycobacteriophage (C2) nucleic acid

1971 ◽  
Vol 17 (2) ◽  
pp. 171-174 ◽  
Author(s):  
Jose Menezes
Keyword(s):  
Nucleic Acid ◽  
Acridine Orange ◽  
Staining Technique ◽  
Fluorescent Staining ◽  
Green Fluorescence ◽  
Acridine Orange Staining ◽  
Fluorescence Characteristic ◽  
Phage Dna ◽  
Acid Dnase

By using the acridine orange staining technique a green fluorescence, characteristic of double-stranded nucleic acid, can be observed with purified preparations of mycobacteriophage C2 and its extracted nucleic acid. DNAse-treated samples do not show this fluorescence, which leads to the conclusion that this fluorescence is associated with phage DNA. Examination of preparations of phage grown in the presence of acridine orange supported these results.

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