Enhancing the conformational stability of growth hormone via site-directed mutagenesis: Incorporation of a metal binding site

Peptides ◽  
1994 ◽  
pp. 1050-1051
Author(s):  
S. R. Lehrman ◽  
M. E. Lund ◽  
J. R. Shifflett
Keyword(s):  
Growth Hormone ◽  
Binding Site ◽  
Metal Binding ◽  
Conformational Stability ◽  
Site Directed Mutagenesis ◽  
Directed Mutagenesis ◽  
Metal Binding Site

scholarly journals Directed Mutagenesis Studies of the Metal Binding Site at the Subunit Interface ofEscherichia coliInorganic Pyrophosphatase

1999 ◽  
Vol 274 (6) ◽  
pp. 3294-3299 ◽  
Author(s):  
Irina S. Efimova ◽  
Anu Salminen ◽  
Pekka Pohjanjoki ◽  
Jukka Lapinniemi ◽  
Natalia N. Magretova ◽  
...  
Keyword(s):  
Binding Site ◽  
Metal Binding ◽  
Directed Mutagenesis ◽  
Metal Binding Site ◽  
Subunit Interface

scholarly journals The second metal-binding site of 70 kDa heat-shock protein is essential for ADP binding, ATP hydrolysis and ATP synthesis

2004 ◽  
Vol 378 (3) ◽  
pp. 793-799 ◽  
Author(s):  
Xueji WU ◽  
Mihiro YANO ◽  
Hiroyo WASHIDA ◽  
Hiroshi KIDO
Keyword(s):  
Heat Shock ◽  
Heat Shock Protein ◽  
Binding Site ◽  
Metal Binding ◽  
Atp Hydrolysis ◽  
Atp Synthesis ◽  
Site Directed Mutagenesis ◽  
Atp Binding ◽  
Binding Motif ◽  
Metal Binding Site

The chaperone activity of Hsp70 (70 kDa heat-shock protein) in protein folding and its conformational switch, including oligomeric and monomeric interconversion, are regulated by the hydrolysis of ATP and the ATP–ADP exchange cycle. The crystal structure of human ATPase domain shows two metal-binding sites, the first for ATP binding and a second, in close proximity to the first, whose function remains unknown [Sriram, Osipiuk, Freeman, Morimoto and Joachimiak (1997) Structure 5, 403–414]. In this study, we have characterized the second metal-binding motif by site-directed mutagenesis and the kinetics of ATP and ADP binding, and found that the second metal-binding site, comprising a loop co-ordinated by His-227, Glu-231 and Asp-232, participates both in ATP hydrolysis and ATP-synthetic activities, in co-operation with the first metal-binding site. The first metal-binding site, a catalytic centre, is essential for ATP binding and the second site for ADP binding in the reactions of ATP hydrolysis and ATP synthesis.

Unraveling the Substrate−Metal Binding Site of Ferrochelatase:  An X-ray Absorption Spectroscopic Study†

Biochemistry ◽  
2002 ◽  
Vol 41 (15) ◽  
pp. 4809-4818 ◽  
Author(s):  
Gloria C. Ferreira ◽  
Ricardo Franco ◽  
Arianna Mangravita ◽  
Graham N. George
Keyword(s):  
Spectroscopic Study ◽  
Binding Site ◽  
Metal Binding ◽  
Metal Binding Site ◽  
X Ray ◽  
Substrate Metal ◽  
X Ray Absorption

Construction of an additional metal-binding site in human metallothionein-2

2006 ◽  
Vol 101 (4) ◽  
pp. 354-360 ◽  
Author(s):  
Mitsutoshi Toyama ◽  
Mariko Sasaki ◽  
Noriaki Hirayama ◽  
Yoshikatsu Murooka ◽  
Mitsuo Yamashita
Keyword(s):  
Binding Site ◽  
Metal Binding ◽  
Metal Binding Site
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