Molecular Biology and Genetic Diversity of Microorganisms

2000 ◽  
pp. 43-57 ◽  
Author(s):  
Vigdis Torsvik ◽  
Frida Lise Daae ◽  
Ruth Anne Sandaa ◽  
Lise Øvreås
Keyword(s):  
Genetic Diversity ◽  
Molecular Biology

KERAGAMAN FENOTIPE DAN GENETIK TIGA VARIETAS KELAPA GENJAH KOPYOR ASAL PATI JAWA TENGAH

2016 ◽  
Vol 21 (1) ◽  
pp. 1 ◽  
Author(s):  
ISMAIL MASKROMO ◽  
ELSJE T. TENDA ◽  
MEITY A. TULALO ◽  
HENGKY NOVARIANTO ◽  
DEWI SUKMA ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Cluster Analysis ◽  
Molecular Biology ◽  
Plant Molecular Biology ◽  
Morphological Diversity ◽  
Local Varieties ◽  
Central Java ◽  
Variety Selection ◽  
Ssr Loci ◽  
Biology Laboratory

<p>ABSTRAK<br />Kelapa Genjah kopyor asal Pati, Jawa Tengah merupakan<br />kekayaaan hayati asli Indonesia dengan nilai ekonomi tinggi. Informasi<br />keragaman genetik kelapa kopyor masih terbatas. Data keragaman<br />morfologi dan genetik diperlukan dalam program pemuliaan kelapa<br />kopyor. Penelitian ini mempelajari keragaman tiga varietas kelapa genjah<br />kopyor asal Pati yang telah dilepas berdasarkan karakter morfologi,<br />kuantitas endosperma, dan keragaman alel marka SSR. Penelitian<br />dilakukan di Pati dan di Laboratorium Plant Molecular Biology,<br />Departemen Agronomi dan Hortikultura, IPB. Evaluasi dilakukan terhadap<br />tiga populasi kelapa Genjah kopyor (hijau, coklat, dan kuning) dengan 30<br />tanaman sampel untuk setiap populasi. Rataan data morfologi digunakan<br />untuk menyusun dendogram. Kuantitas endosperma diamati pada satu<br />buah kelapa kopyor per tanaman yang dievaluasi. Karakteristik<br />endosperma dikelompokkan sesuai kategori yang telah ditetapkan. Untuk<br />setiap populasi, analisis marka dengan lima pasang primer SSR dilakukan<br />pada 10 tanaman sampel. Data yang didapat digunakan untuk menentukan<br />keragaman genetik kelapa Genjah kopyor asal Pati. Hasil pengamatan<br />menunjukkan keragaman morfologis dan alel SSR antar tanaman dalam<br />varietasnya (keragaman intra-varietas) rendah. Sebaliknya, keragaman<br />morfologis dan alel SSR antar varietasnya tinggi. Kuantitas endosperma<br />kelapa Genjah kopyor asal Pati bervariasi antara skor 1–6. Keragaman<br />genetik yang rendah dalam varietas dan tinggi antar ketiga varietas (coklat,<br />hijau, dan kuning) memperkuat pelepasan ketiganya sebagai varietas lokal.<br />Selain itu, keragaman genetik antar tanaman dalam varietas yang rendah<br />mendukung penggunaan ketiga varietas lokal sebagai tetua dalam program<br />perakitan varietas kelapa kopyor unggul baru. Tetua yang dipilih dapat<br />diseleksi intra-varietas berdasarkan persentase buah kopyor per tandan<br />dan skor kuantitas endosperma yang tinggi.<br />Kata kunci: Keragaman morfologis, keragaman intra dan antar varietas,<br />kuantitas endosperma</p><p>ABSTRACT<br />Kopyor dwarf coconuts are mutants from Pati, Central Java having<br />high economic values. However, morphological and genetic diversities of<br />this coconut were still limited. Morphological and genetic diversity data<br />are needed for breeding program. The research objectives were to evaluate<br />intra and inter-specific diversity based on morphology, endosperm<br />quantity, and SSR alleles. Field evaluations were conducted in Pati while<br />laboratory activities were at Plant Molecular Biology Laboratory,<br />Department of Agronomy and Horticulture, IPB. Three populations of<br />kopyor dwarf varieties (brown, green, and yellow) were evaluated. Thirty<br />trees were sampled for each population. The average of morphological<br />data were used to construct cluster analysis. Endosperm quantity was<br />scored (0 – 9) based on a single nut sample. Ten palms were analyzed<br />using five SSR loci for each population and used to determine genetic<br />diversity of populations. Results of observations indicated intra-variety<br />morphological and SSR allele variations among kopyor dwarf was low.<br />However, inter-variety variations were high. The endosperm quantity<br />scores among kopyor dwarf coconut ranged from 1–6. The low intra-<br />variety and high inter-variety variations among the three kopyor dwarf<br />coconut supported their release as different local varieties. Moreover, the<br />low intra-variety phenotypic and genotypic diversities among kopyor<br />brown, green, and yellow dwarf coconut support their use as parents for<br />new and superior kopyor coconut variety development in the future. For<br />such purpose, however, it is necessary to conduct intra-variety selection to<br />identify desirable parents based on high kopyor fruit percentage per bunch<br />and for high kopyor endosperm quantity.<br />Key words: Morphological diversity, intra and inter variety diversities,<br />quantity, endosperm</p>

Genetic diversity of HIV-1 subtype F from Brazil: failure of HIV-1 viral load testing based on molecular biology amplification methods

AIDS ◽  
1999 ◽  
Vol 13 (15) ◽  
pp. 2183 ◽  
Author(s):  
João Marcos M. Mercado ◽  
Renato Di Dio ◽  
Mário G. Pradal
Keyword(s):  
Genetic Diversity ◽  
Viral Load ◽  
Molecular Biology ◽  
Load Testing ◽  
Viral Load Testing ◽  
Hiv 1

scholarly journals GENETIC DIVERSITY OF BACTERIAL WILT CAUSED BY RALSTONIA SOLANACEARUM AS ASSESSED BY PCR

2017 ◽  
Vol 29 (1) ◽  
pp. 69
Author(s):  
Muhammad Mansoor ◽  
Muhammad W. Abbas ◽  
J. D V. Elsas ◽  
Muhammad R. Bashir ◽  
Muhammad Atiq
Keyword(s):  
Genetic Diversity ◽  
Molecular Biology ◽  
Bacterial Wilt ◽  
Ralstonia Solanacearum ◽  
Wilt Disease ◽  
Multi Locus Sequence Typing ◽  
Chain Reaction ◽  
Bacterial Wilt Disease ◽  
Different Strains ◽  
Modern Tool

The current research was conducted to investigate genetic diversity of Ralstonia solanacearum for comparison of different strains that were collected mainly from Netherlands as well as from Bangladesh, Brazil, Kenya, Egypt, Pakistan and Palma. Forty six strains were included in contemporary studies whereas main biovars for these strains included biovar-2 except GMI1000 that belonged to biovar 3. Genetic diversity of bacterial wilt disease caused by R. solancearum was assessed by focusing mainly on three genes i.e. mutL, cbhA and dps. All the genes seem to be conserved but in case of mutL some strains showed divergence. Multi Locus Sequence Typing (MLST) scheme was used in this contemporary research. It was concluded that polymerized chain reaction (PCR) is the most imperative and appropriate modern tool of molecular biology to find genetic diversity in Ralstonia solanacearum causing bacterial wilt.     

Santalum molecular biology: molecular markers for genetic diversity, phylogenetics and taxonomy, and genetic transformation

2017 ◽  
Vol 92 (5) ◽  
pp. 1301-1315 ◽  
Author(s):  
Jaime A. Teixeira da Silva ◽  
Mafatlal M. Kher ◽  
Deepak Soner ◽  
M. Nataraj ◽  
Judit Dobránszki ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Molecular Markers ◽  
Molecular Biology ◽  
Genetic Transformation

scholarly journals Molecular Pharmacognosy: A new Borderline Discipline

2009 ◽  
Vol 4 (11) ◽  
pp. 1934578X0900401
Author(s):  
Lu-Qi Huang ◽  
Yuan Yuan ◽  
Guang-Hong Cui ◽  
Zhu-Bo Dai ◽  
Pei-Gen Xiao
Keyword(s):  
Genetic Diversity ◽  
Molecular Biology ◽  
Genetic Engineering ◽  
Molecular Marker ◽  
Active Compound ◽  
Interdisciplinary Science ◽  
Wide Range ◽  
Wild Resources ◽  
Crude Drugs ◽  
Marker Assay

Pharmacognosy has developed rapidly in recent years and now represents a highly interdisciplinary science. At the boundary between pharmacognosy and molecular biology, molecular pharmacognosy has developed as a new borderline discipline. Using the method and technology of molecular biology, molecular pharmacognosy focuses on resolving a wide range of challenging problems, such as distinguishing herbal and animal drug populations by molecular marker assay, conserving and utilizing wild resources on the basis of knowledge of genetic diversity, investigating the mechanism of active compound accumulation and obtaining new resources with higher quality through genetic engineering. Recent research results show that molecular pharmacognosy has extended the scope of pharmacognostical science and plays an important role in the safe and efficient usage of crude drugs.

scholarly journals Saving living diversity in the face of the unstoppable 6th mass extinction: a call for urgent international action

2017 ◽  
Vol 1 (2) ◽  
Author(s):  
Fred Naggs
Keyword(s):  
Genetic Diversity ◽  
Molecular Biology ◽  
Mass Extinction ◽  
Human Population ◽  
Biological Diversity ◽  
Low Cost ◽  
Global Scale ◽  
Numerous Species ◽  
Damage Limitation ◽  
The Face

The global scale and impact of current and increasing human population size is incompatible with the survival of biological diversity and the 6th mass extinction cannot be stopped. For the vast majority of species we have neither the knowledge of when they will go extinct nor the capacity to find out. Conventional conservation measures can only amount to token damage limitation. Advances in molecular biology allow low cost options for storing the genetic diversity of numerous species and maximising future options for restoring species.

Recent progress in henipavirus research: Molecular biology, genetic diversity, animal models

2012 ◽  
Vol 95 (2) ◽  
pp. 135-149 ◽  
Author(s):  
Barry Rockx ◽  
Richard Winegar ◽  
Alexander N. Freiberg
Keyword(s):  
Genetic Diversity ◽  
Animal Models ◽  
Molecular Biology ◽  
Recent Progress
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