Mutation and selection for improved oil and meal quality in Brassica napus utilizing microspore culture

Author(s):  
L. Kott ◽  
R. Wong ◽  
E. Swanson ◽  
J. Chen
2012 ◽  
Vol 38 (No. 1) ◽  
pp. 50-54 ◽  
Author(s):  
V. Kučera ◽  
M. Vyvadilová ◽  
M. Klíma

A survey of development and prospects of the utilisation of doubled haploid techniques in rapeseed breeding in the world and in the Czech Republic is presented. The first utilisation of spontaneously occurred haploids from Brassica napus inbreeding programmes is described. The development of techniques of anther and later microspore culture is outlined. Special emphasis is given to the practical use of doubled haploids for the improvement of the effectiveness of breeding new productive cultivars. Some partial results of evaluation of yield parameters and resistance to important diseases in the obtained doubled haploid lines of winter oilseed rape are shown. The literary review and present results indicate, that the doubled haploid technique can be effectively used for the development of homozygous oilseed rape lines as an alternative to conventional methods.


Genome ◽  
1995 ◽  
Vol 38 (2) ◽  
pp. 414-416 ◽  
Author(s):  
P. K. Tanhuanpää ◽  
J. P. Vilkki ◽  
H. J. Vilkki

The F2 progeny (64 individuals) from the cross between oilseed rape (Brassica napus L.) cultivar Topas and R4 (a low linolenic mutation line) was analyzed with 8 RFLPs and 34 RAPDs to discover a genetic tag for gene(s) affecting linolenic acid concentration. According to variance analysis (ANOVA), one RAPD marker (25a) was significantly associated with linolenic acid content; the linolenic acid concentration in the seeds of F2 individuals showing the marker (includes both homo- and hetero-zygotes) was 7.43 ± 1.35% and in those lacking the marker was 5.70 ± 1.52%. Marker 25a may be used to facilitate selection for fatty acid composition in future breeding programs of oilseed rape.Key words: Brassica napus, RFLP, RAPD, linolenic acid.


Genes ◽  
2020 ◽  
Vol 11 (10) ◽  
pp. 1161
Author(s):  
Linh Bao Ton ◽  
Ting Xiang Neik ◽  
Jacqueline Batley

Since their domestication, Brassica oilseed species have undergone progressive transformation allied with the development of breeding and molecular technologies. The canola (Brassica napus) crop has rapidly expanded globally in the last 30 years with intensive innovations in canola varieties, providing for a wider range of markets apart from the food industry. The breeding efforts of B. napus, the main source of canola oil and canola meal, have been mainly focused on improving seed yield, oil quality, and meal quality along with disease resistance, abiotic stress tolerance, and herbicide resistance. The revolution in genetics and gene technologies, including genetic mapping, molecular markers, genomic tools, and gene technology, especially gene editing tools, has allowed an understanding of the complex genetic makeup and gene functions in the major bioprocesses of the Brassicales, especially Brassica oil crops. Here, we provide an overview on the contributions of these technologies in improving the major traits of B. napus and discuss their potential use to accomplish new improvement targets.


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