Association of a RAPD marker with linolenic acid concentration in the seed oil of rapeseed (Brassica napus L.)

Genome ◽  
1995 ◽  
Vol 38 (2) ◽  
pp. 414-416 ◽  
Author(s):  
P. K. Tanhuanpää ◽  
J. P. Vilkki ◽  
H. J. Vilkki

The F2 progeny (64 individuals) from the cross between oilseed rape (Brassica napus L.) cultivar Topas and R4 (a low linolenic mutation line) was analyzed with 8 RFLPs and 34 RAPDs to discover a genetic tag for gene(s) affecting linolenic acid concentration. According to variance analysis (ANOVA), one RAPD marker (25a) was significantly associated with linolenic acid content; the linolenic acid concentration in the seeds of F2 individuals showing the marker (includes both homo- and hetero-zygotes) was 7.43 ± 1.35% and in those lacking the marker was 5.70 ± 1.52%. Marker 25a may be used to facilitate selection for fatty acid composition in future breeding programs of oilseed rape.Key words: Brassica napus, RFLP, RAPD, linolenic acid.

1976 ◽  
Vol 56 (4) ◽  
pp. 961-966 ◽  
Author(s):  
Z. P. KONDRA ◽  
T. W. WILSON

Single plant selections for high, intermediate, and low oleic, linoleic and linolenic acid content of rapeseed (Brassica napus L.) oil were made in the F2 generation of three crosses. The three parent strains produced seed oil practically free of eicosenoic and erucic acids and differed in oleic, linoleic and linolenic acid content. The F3 and F4 progeny were analyzed for fatty acid composition. Selection for low and high values of oleic and linoleic acid content was reasonably successful in two out of three crosses. Selection for extremes of linolenic acid content was unsuccessful in all three crosses. Regression analyses indicated high heritabilities for oleic and linoleic acids in two of three crosses but low heritabilities for linolenic acid in all three crosses. Correlations between oleic versus linoleic and oleic versus linolenic were negative and highly significant. Correlations between linoleic versus linolenic were of lower magnitude but positive and significant in all but two populations.


1997 ◽  
Vol 77 (1) ◽  
pp. 125-126 ◽  
Author(s):  
R. Scarth ◽  
P. B. E. McVetty ◽  
S. R. Rimmer

Allons (Brassica napus L.) is a low linolenic acid canola cultivar which produces seed oil with an average linolenic acid content of 2.5%. The low linolenic oil has a shorter hydrogenation time and greater stability than standard canola oil. Allons is higher yielding and contains higher seed oil and lower meal protein than the low linolenic cultivar Stellar. Allons is adapted to the long season B. napus canola growing areas of western Canada. Key words: Rape (summer), low linolenic, cultivar description


2021 ◽  
Vol 22 (3) ◽  
pp. 1033
Author(s):  
Abirami Rajavel ◽  
Selina Klees ◽  
Johanna-Sophie Schlüter ◽  
Hendrik Bertram ◽  
Kun Lu ◽  
...  

Transcription factors (TFs) and their complex interplay are essential for directing specific genetic programs, such as responses to environmental stresses, tissue development, or cell differentiation by regulating gene expression. Knowledge regarding TF–TF cooperations could be promising in gaining insight into the developmental switches between the cultivars of Brassica napus L., namely Zhongshuang11 (ZS11), a double-low accession with high-oil- content, and Zhongyou821 (ZY821), a double-high accession with low-oil-content. In this regard, we analysed a time series RNA-seq data set of seed tissue from both of the cultivars by mainly focusing on the monotonically expressed genes (MEGs). The consideration of the MEGs enables the capturing of multi-stage progression processes that are orchestrated by the cooperative TFs and, thus, facilitates the understanding of the molecular mechanisms determining seed oil content. Our findings show that TF families, such as NAC, MYB, DOF, GATA, and HD-ZIP are highly involved in the seed developmental process. Particularly, their preferential partner choices as well as changes in their gene expression profiles seem to be strongly associated with the differentiation of the oil content between the two cultivars. These findings are essential in enhancing our understanding of the genetic programs in both cultivars and developing novel hypotheses for further experimental studies.


2005 ◽  
Vol 107 (3) ◽  
pp. 180-186 ◽  
Author(s):  
Juan Carlos López-Martínez ◽  
Pablo Campra-Madrid ◽  
Miguel Ángel Rincón-Cervera ◽  
José Luis Guil-Guerrero

1994 ◽  
Vol 74 (2) ◽  
pp. 275-277 ◽  
Author(s):  
L. A. Murphy ◽  
R. Scarth

Early maturity is a major objective of oilseed rape (Brassica napus L.) breeding programs in western Canada. Maturity of crops is influenced by time of initiation and flowering. The presence of a vernalization requirement affects plant development by delaying floral initiation until the cold requirement of the plant has been satisfied. Five spring oilseed rape cultivars were screened for their response to vernalization. Vernalization treatments consisted of exposure of germinated seeds to 0–42 d at 4 °C. Plants were assessed under a 20-h photoperiod. In general, there was a cumulative response to vernalization, with a decrease in days to each developmental stage as exposure to 4 °C was increased. Vernalization treatment of 6 d at 4 °C was sufficient to decrease both the days to first flower and the final leaf number. The characterization of vernalization response is of interest because variation in flowering time in response to year-to-year variations in the environment could result. Key words:Brassica napus, canola, oilseed rape, vernalization


1990 ◽  
Vol 93 (2) ◽  
pp. 418-424 ◽  
Author(s):  
D. Evan Evans ◽  
Joseph P. Sang ◽  
Xenophon Cominos ◽  
Neil E. Rothnie ◽  
R. Bruce Knox

PLoS ONE ◽  
2020 ◽  
Vol 15 (6) ◽  
pp. e0233959 ◽  
Author(s):  
Stanisław Spasibionek ◽  
Katarzyna Mikołajczyk ◽  
Hanna Ćwiek–Kupczyńska ◽  
Teresa Piętka ◽  
Krystyna Krótka ◽  
...  

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