Rapid Electrokinetic Patterning and Its Applications

2016 ◽  
pp. 3409-3419
Author(s):  
Katherine Clayton ◽  
Jian-Wei Khor ◽  
Steven T. Wereley
Keyword(s):  
Rapid Electrokinetic Patterning

Towards New Methodologies for Manipulation of Colloidal Particles in a Miniaturized Fluidic Device: Optoelectrokinetic Manipulation Technique

2013 ◽  
Vol 135 (2) ◽  
Author(s):  
Jae-Sung Kwon ◽  
Steven T. Wereley
Keyword(s):  
Microfluidic Chip ◽  
Colloidal Particles ◽  
Future Prospects ◽  
Simultaneous Application ◽  
Laser Illumination ◽  
Research Fields ◽  
Ac Electric Field ◽  
Fluidic Device ◽  
New Methodologies ◽  
Rapid Electrokinetic Patterning

The rapid electrokinetic patterning (REP) technique developed recently is a hybrid optoelectrokinetic one that manipulates micro- or nanocolloids in a microfluidic chip using the simultaneous application of a uniform ac electric field and laser illumination. Since its invention, the technique has been applied to many research fields with promising potential, but these applications are still in their early stages. In order to effectively complete and leverage the applications, this paper reviews the publications concerning the REP technique and discusses its underlying principles, applications, and future prospects.

Optically Induced Electrokinetic Trapping and Sorting of Colloids

2010 ◽  
Author(s):  
Stuart J. Williams ◽  
Aloke Kumar ◽  
Steven T. Wereley
Keyword(s):  
Electrical Conductivity ◽  
Surface Modification ◽  
Particle Diameter ◽  
Lab On A Chip ◽  
Particle Manipulation ◽  
Complete Understanding ◽  
Heating Source ◽  
Electrokinetic Trapping ◽  
Rapid Electrokinetic Patterning ◽  
Optically Induced

Recently, we have demonstrated electrothermal hydrodynamics with an external heating source of a highly focused 1,064 nm laser beam [1]. This phenomenon, when coupled with particle-electrode electrokinetic interactions, has led to the rapid and selective concentration of suspended colloids [2–6]. This technique, termed Rapid Electrokinetic Patterning (REP) was demonstrated without any additional surface modification or patterning of the electrodes. This dynamic, optically induced fluid and particle manipulation technique could be used for a variety of lab-on-a-chip applications. However, there are additional effects that have yet to be investigated that are important for a complete understanding of REP. This paper showcases experimental particle-particle behavior observations by varying particle diameter, electrode material, and preliminary results of varying fluid electrical conductivity.

Development of a Bead-Based Optoelectrokinetic Immunosensing Technique for Detection of Low-Abundance Analytes

2017 ◽  
Author(s):  
Han-Sheng Chuang ◽  
Hsiao-Neng Lin ◽  
Hu-Yao Ku
Keyword(s):  
Limit Of Detection ◽  
Point Of Care ◽  
Particle Manipulation ◽  
Great Flexibility ◽  
Point Of Care Diagnostics ◽  
Combined Use ◽  
Tnf Α ◽  
Rapid Electrokinetic Patterning ◽  
Sample Droplet ◽  
Made In

Bead-based immunosensing has been growing as a promising technology in the point-of-care diagnostics because of great flexibility. For dilute samples, functionalized particles can be used to collect dispersed analytes and act as carriers for particle manipulation. To carry out rapid and selective diagnosis, a bead-based optoelectrokinetic immunosensing technique was developed herein to detect biomarkers, lipocalin 1 (LCN1) and TNF-α, for diabetic retinopathy (DR). The measurement was made in a sample droplet sandwiched between two parallel electrodes. With an electric field and a focused laser beam simultaneously applying on the microchip, the immunocomplexes in the droplet were further concentrated within the region of irradiation to enhance the fluorescent signal. The optoelectrokinetic platform, termed rapid electrokinetic patterning (REP), is excellent in dynamic and programmable particle manipulation. Therefore, the detection could be complete in roughly 10 s. With an appropriate frequency modulation, the two DR biomarkers were detected at a time. The limit of detection (LOD) of the REP-enabled measurement reached as low as 100 pg/mL. The combined use of bead-based immunoassays and the optoelectrokinetic platform therefore provides an insightful measure to the early diagnosis of diseases.

Rapid Electrokinetic Patterning and Its Applications

2015 ◽  
pp. 1-11
Author(s):  
Katherine Clayton ◽  
Jian-Wei Khor ◽  
Steven T. Wereley
Keyword(s):  
Rapid Electrokinetic Patterning

Electrothermal Pumping With Thin Film Resistive Heaters

2012 ◽  
Author(s):  
Andrew Work ◽  
Vanessa Velasco ◽  
Stuart J. Williams
Keyword(s):  
Thin Film ◽  
Tin Oxide ◽  
Indium Tin Oxide ◽  
Parallel Plate ◽  
Colloidal Particles ◽  
Infrared Laser ◽  
Electrode Geometry ◽  
Ac Electric Field ◽  
Glass Slides ◽  
Rapid Electrokinetic Patterning

Rapid Electrokinetic Patterning (REP) is a technique used to gather suspended colloidal particles at a planar electrode surface. REP is capable of gathering colloids using very simplistic electrode geometry. Two parallel plate electrodes are used to supply an AC electric field, and an infrared laser is required to heat the surface. Gold and indium tin oxide (ITO) on glass slides have been used as electrode material. Experiments were conducted using ITO coated glass slides, a 980 nm infrared laser, one-micron red fluorescent polystyrene particles, and an aqueous KCl solution. An inverted Nikon Eclipse microscope was used, and video was captured using a PCO Sensicam camera.

Optically Induced Rapid Electrokinetic Patterning: A Study of the Operational Regimes and Dominant Forces

2009 ◽  
Author(s):  
Aloke Kumar ◽  
Steven T. Wereley
Keyword(s):  
Colloidal Particle ◽  
Particle Density ◽  
Trap Particle ◽  
Laser Beams ◽  
Power Laser ◽  
Induced Dipole ◽  
Patterning Technique ◽  
Rapid Electrokinetic Patterning ◽  
Optically Induced

We study a recently proposed optically induced electrokinetic colloidal particle patterning technique that can pattern/translate or trap particle clusters on an electrode surface using high power laser beams and under an applied AC field. By exploring variations in particle density, with changes in various factors, using Voronoi tessellations we show that the role of various forces changes dramatically with conductivity. For low conductivity (∼2 mS/m) solutions we show that particle density increases with frequency primarily due to a lowering of the induced dipole moment of the particle.

Optically Induced Rapid Electrokinetic Patterning of Non-Spherical Particles: Study of Colloidal Phase Transition

2010 ◽  
Author(s):  
Raviraj Vijay Thakur ◽  
Steven Wereley
Keyword(s):  
Phase Transition ◽  
Fluid Mechanics ◽  
Electrode Surface ◽  
Colloidal Particles ◽  
Spherical Particles ◽  
Lab On Chip ◽  
Chip Technology ◽  
Sorting Technique ◽  
On Chip ◽  
Rapid Electrokinetic Patterning

Patterning of colloidal particles on surfaces is an application that has evinced wide interest from the fluid mechanics community, due to its possible applicability in a number of engineering situations such as manufacture of photonic crystals[1], bioengineering tissues[2] and lab on chip technology[3], etc. Recently Kumar et al. had proposed the technique of rapid electrokinetic patterning (REP) [4], a hybrid opto-electric manipulation technique that can manipulate and pattern colloidal particles on an electrode surface. REP utilizes optical landscapes to create local gradients in temperature on an electrode substrate. This allows local changes in permittivity and conductivity of the fluid. Colloidal particles can then be dynamically patterned at the illuminated locations of the electrode surface. REP can be used for capturing selective group of particles and thus it serves as a sorting technique too [5].

Advances and Applications of Rapid Electrokinetic Patterning

2018 ◽  
Vol 98 (2) ◽  
pp. 85-101 ◽  
Author(s):  
Mohamed Z. Rashed ◽  
Vanessa Velasco ◽  
Stuart J. Williams
Keyword(s):  
Rapid Electrokinetic Patterning

scholarly journals Sensitive tear screening of diabetic retinopathy with dual biomarkers enabled using a rapid electrokinetic patterning platform

Lab on a Chip ◽  
2020 ◽  
Vol 20 (2) ◽  
pp. 356-362 ◽  
Author(s):  
Jen-Yi Wang ◽  
Jae-Sung Kwon ◽  
Sheng-Min Hsu ◽  
Han-Sheng Chuang
Keyword(s):  
Quality Of Life ◽  
Diabetic Retinopathy ◽  
Early Treatment ◽  
Diagnostic Method ◽  
Screening Technique ◽  
Rapid Electrokinetic Patterning

A screening technique with dual biomarkers for diabetic retinopathy (DR) based on optoelectrokinetics was presented. Our technique will revolutionize the diagnostic method for DR and enable an early treatment to improve patients' quality of life.

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