Environmental Adaptation of Dihydrofolate Reductase from Deep-Sea Bacteria

2015 ◽  
pp. 423-442 ◽  
Author(s):  
Eiji Ohmae ◽  
Kunihiko Gekko ◽  
Chiaki Kato
Keyword(s):  
Dihydrofolate Reductase ◽  
Deep Sea ◽  
Environmental Adaptation ◽  
Deep Sea Bacteria

scholarly journals 3P043 Structural Characteistic of Dihydrofolate Reductase from Deep-sea Bacteria by High Pressure NMR

2005 ◽  
Vol 45 (supplement) ◽  
pp. S214
Author(s):  
K. Hata ◽  
Xu Ying ◽  
E. Ohmae ◽  
K. Gekko ◽  
R. Kitahara ◽  
...  
Keyword(s):  
High Pressure ◽  
Dihydrofolate Reductase ◽  
Deep Sea ◽  
Deep Sea Bacteria

scholarly journals Chemical Elicitors Induce Rare Bioactive Secondary Metabolites in Deep-Sea Bacteria under Laboratory Conditions

Metabolites ◽  
2021 ◽  
Vol 11 (2) ◽  
pp. 107
Author(s):  
Rafael de Felício ◽  
Patricia Ballone ◽  
Cristina Freitas Bazzano ◽  
Luiz F. G. Alves ◽  
Renata Sigrist ◽  
...  
Keyword(s):  
Natural Products ◽  
Secondary Metabolites ◽  
Natural Product ◽  
Deep Sea ◽  
Chemical Space ◽  
Bacterial Genome ◽  
Vast Number ◽  
Bacterial Metabolites ◽  
Laboratory Conditions ◽  
Deep Sea Bacteria

Bacterial genome sequencing has revealed a vast number of novel biosynthetic gene clusters (BGC) with potential to produce bioactive natural products. However, the biosynthesis of secondary metabolites by bacteria is often silenced under laboratory conditions, limiting the controlled expression of natural products. Here we describe an integrated methodology for the construction and screening of an elicited and pre-fractionated library of marine bacteria. In this pilot study, chemical elicitors were evaluated to mimic the natural environment and to induce the expression of cryptic BGCs in deep-sea bacteria. By integrating high-resolution untargeted metabolomics with cheminformatics analyses, it was possible to visualize, mine, identify and map the chemical and biological space of the elicited bacterial metabolites. The results show that elicited bacterial metabolites correspond to ~45% of the compounds produced under laboratory conditions. In addition, the elicited chemical space is novel (~70% of the elicited compounds) or concentrated in the chemical space of drugs. Fractionation of the crude extracts further evidenced minor compounds (~90% of the collection) and the detection of biological activity. This pilot work pinpoints strategies for constructing and evaluating chemically diverse bacterial natural product libraries towards the identification of novel bacterial metabolites in natural product-based drug discovery pipelines.

scholarly journals Contribution of trimethylamine N-oxide on the growth and pressure tolerance of deep-sea bacteria

2018 ◽  
Vol 37 (1) ◽  
pp. 210-222 ◽  
Author(s):  
Qunjian Yin ◽  
Weijia Zhang ◽  
Xuegong Li ◽  
Lihong Zhou ◽  
Xiaoqing Qi ◽  
...  
Keyword(s):  
Deep Sea ◽  
Deep Sea Bacteria ◽  
Pressure Tolerance

Environmental adaptation: Genomic analysis of the piezotolerant and psychrotolerant deep-sea iron reducing bacterium Shewanella piezotolerans WP3

2008 ◽  
Vol 136 ◽  
pp. S542-S543
Author(s):  
Fengping Wang ◽  
Jianbin Wang ◽  
Huahua Jian ◽  
Bing Zhang ◽  
Feng Wang ◽  
...  
Keyword(s):  
Deep Sea ◽  
Genomic Analysis ◽  
Environmental Adaptation

Deep-Sea Bacteria

2000 ◽  
pp. 161-174 ◽  
Author(s):  
A. Aristides Yayanos
Keyword(s):  
Deep Sea ◽  
Deep Sea Bacteria

Lipids: Fatty Acids

2011 ◽  
Author(s):  
Thomas S. Bianchi ◽  
Elizabeth A. Canuel
Keyword(s):  
Fatty Acids ◽  
Polyunsaturated Fatty Acids ◽  
Deep Sea ◽  
Saturated Fatty Acids ◽  
Relative Proportion ◽  
Aquatic Organisms ◽  
Building Blocks ◽  
Deep Sea Bacteria ◽  
Total Fatty Acids ◽  
Fatty Acid Biomarkers

This chapter discusses fatty acids, the building blocks of lipids, which represent a significant fraction of the total lipid pool in aquatic organisms. It explores how chain length and levels of unsaturation (number of double bonds) have been shown to be correlated to decomposition, indicating a pre- and postdepositional selective loss of short-chain and polyunsaturated fatty acids. In contrast, saturated fatty acids are more stable and typically increase in relative proportion to total fatty acids with increasing sediment depth. Polyunsaturated fatty acids (PUFAs) are predominantly used as proxies for the presence of “fresh” algal sources, although some PUFAs also occur in vascular plants and deep-sea bacteria. Thus, these biomarkers represent a very diverse group of compounds present in aquatic systems. The numerous applications of fatty acid biomarkers to identifying the sources of organic matter in lakes, rivers, estuaries, and marine ecosystems are discussed.

scholarly journals Characterization of Two Toxin-Antitoxin Systems in Deep-Sea Streptomyces sp. SCSIO 02999

Marine Drugs ◽  
2019 ◽  
Vol 17 (4) ◽  
pp. 211 ◽  
Author(s):  
Waner Zhan ◽  
Jianyun Yao ◽  
Kaihao Tang ◽  
Yangmei Li ◽  
Yunxue Guo ◽  
...  
Keyword(s):  
Toxic Effect ◽  
Deep Sea ◽  
Pathogenic Bacteria ◽  
Direct Interaction ◽  
Streptomyces Sp ◽  
E Coli ◽  
Deep Sea Bacteria ◽  
Bona Fide ◽  
Ta Studies

Toxin-antitoxin (TA) systems are ubiquitous and abundant genetic elements in bacteria and archaea. Most previous TA studies have focused on commensal and pathogenic bacteria, but have rarely focused on marine bacteria, especially those isolated from the deep sea. Here, we identified and characterized three putative TA pairs in the deep-sea-derived Streptomyces sp. strain SCSIO 02999. Our results showed that Orf5461/Orf5462 and Orf2769/Orf2770 are bona fide TA pairs. We provide several lines of evidence to demonstrate that Orf5461 and Orf5462 constitute a type-II TA pair that are homologous to the YoeB/YefM TA pair from Escherichia coli. Although YoeB from SCSIO 02999 was toxic to an E. coli host, the homologous YefM antitoxin from SCSIO 02999 did not neutralize the toxic effect of YoeB from E. coli. For the Orf2769/Orf2770 TA pair, Orf2769 overexpression caused significant cell elongation and could lead to cell death in E. coli, and the neighboring Orf2770 could neutralize the toxic effect of Orf2769. However, no homologous toxin or antitoxin was found for this pair, and no direct interaction was found between Orf2769 and Orf2770. These results suggest that Orf2769 and Orf2770 may constitute a novel TA pair. Thus, deep-sea bacteria harbor typical and novel TA pairs. The biochemical and physiological functions of different TAs in deep-sea bacteria warrant further investigation.

scholarly journals Screening and Phylogenetic Analysis of Deep-Sea Bacteria Capable of Metabolizing Lignin-Derived Aromatic Compounds

2012 ◽  
Vol 02 (04) ◽  
pp. 177-187 ◽  
Author(s):  
Y. Ohta ◽  
S. Nishi ◽  
T. Haga ◽  
T. Tsubouchi ◽  
R. Hasegawa ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
Aromatic Compounds ◽  
Deep Sea ◽  
Deep Sea Bacteria

scholarly journals Deep-sea bacteria enriched by oil and dispersant from the Deepwater Horizon spill

2012 ◽  
Vol 14 (9) ◽  
pp. 2405-2416 ◽  
Author(s):  
Jacob Baelum ◽  
Sharon Borglin ◽  
Romy Chakraborty ◽  
Julian L. Fortney ◽  
Regina Lamendella ◽  
...  
Keyword(s):  
Deep Sea ◽  
Deepwater Horizon ◽  
Deep Sea Bacteria
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