Decellularized Tissue Engineering

2017 ◽  
pp. 185-226 ◽  
Author(s):  
Nana Shirakigawa ◽  
Hiroyuki Ijima
Keyword(s):  
Tissue Engineering ◽  
Decellularized Tissue

Tissue Engineering with Natural Tissue Matrices

2010 ◽  
Vol 76 ◽  
pp. 125-132 ◽  
Author(s):  
Akio Kishida ◽  
Seiichi Funamoto ◽  
Jun Negishi ◽  
Yoshihide Hashimoto ◽  
Kwangoo Nam ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Blood Vessel ◽  
Heart Valve ◽  
Biomechanical Properties ◽  
Biological Tissues ◽  
The Novel ◽  
Detergent Treatment ◽  
Decellularized Tissue ◽  
Natural Tissue ◽  
Porcine Tissues

Natural tissue, especially autologous tissue is one of ideal materials for tissue regeneration. Decellularized tissue could be assumed as a second choice because the structure and the mechanical properties are well maintained. Decellularized human tissues, for instance, heart valve, blood vessel, and corium, have already been developed and applied clinically. Nowadays, decellularized porcine tissues are also investigated. These decellularized tissues were prepared by detergent treatment. The detergent washing is easy but sometime it has problems. We have developed the novel decellularization method, which applied the high-hydrostatic pressure (HHP). As the tissue set in the pressurizing chamber is treated uniformly, the effect of the high-hydrostatic pressurization does not depend on the size of tissue. We have reported the HHP decellularization of heart valve, blood vessel, bone, and cornea. Furthermore, HHP treatments are reported to have the ability of the extinction of bacillus and the inactivation of virus. So, the HHP treatment is also expected as the sterilization method. We are investigating efficient processes of decellularization and recellularization of biological tissues to have bioscaffolds keeping intact structure and biomechanical properties. Our recent studies on tissue engineering using HHP decellularized tissue will be reported here.

Repopulation of decellularized pig scaffolds: Promising approach for liver tissue engineering

2019 ◽  
Vol 98 (10) ◽  
Keyword(s):  
Tissue Engineering ◽  
Cell Adhesion ◽  
Liver Tissue ◽  
Host Organism ◽  
Spatial Cues ◽  
Liver Tissue Engineering ◽  
3D Environment ◽  
Ecm Proteins ◽  
Decellularized Tissue ◽  
Decellularized Liver

Repopulation of decellularized tissue with cells is a very promising approach in tissue engineering, with liver tissue engineering not being an exception. Decellularized liver scaffolds can serve as an excellent 3D environment for recellularization as it maintain tissue-specific microarchitecture of ECM proteins with important spatial cues for cell adhesion, migration, growth and differentiation. Moreover, by using autologous cells the newly constructed graft should lack immunogenicity in the host organism and thus eliminate the need for immunosuppressive therapy in the post-transplant period. This review provides an overview of liver decellularization and repopulation experiments done so far while highlighting the advances as well as pin-pointing the challenges that remain to be solved.

scholarly journals Review on Brain Decellularization Methods and their Applications for Tissue Engineering

2020 ◽  
Author(s):  
Leila Darabi ◽  
Farshad Homayouni Moghadam ◽  
Mohammad Hossein Nasr Esfahani
Keyword(s):  
Tissue Engineering ◽  
Brain Tissue ◽  
Brain Injuries ◽  
Regulatory Function ◽  
Secretory Product ◽  
Limited Capacity ◽  
Growth And Survival ◽  
Physical Chemical ◽  
Decellularized Tissue ◽  
The Brain

Introduction: Tissue engineering by using decellularized tissues has been attracted attention of researchers in the regenerative medicine. Extra cellular matrix (ECM) is a secretory product of cells inside the tissues with supportive and regulatory function for homing cells. ECM contains glycosaminoglycans (GAGs) and fibrous proteins. Each particular tissue has its unique ECM, especially brain, because of its limited capacity for renovation, which is noticeable during aging and brain injuries. Recent studies reported that decellularized brain could provide necessary ECM for growth and survival of neurons. The main available decellularization techniques are based on physical, chemical and enzymatic approaches. Regarding the fragility of brain tissue, decellularization methods have been optimized to three methods: detergent, detergent enzymatic and physicochemical-enzymatic methods. Focusing on these methods, we performed this review to compare the efficacy and functionality of brain decellularization methods. Conclusion: The decellularized tissue of the brain contains a variety of glycoprotein components that can be used in the preparation of engineered scaffolds for the survival of nerve cells as well as in the preparation of brain organoids. Brain tissue decellularization has been much more successful with the methods that use the chemical solvents Triton X100, trypsin, and DNase in combination with freeze-thaw cycles and low-speed centrifuges.

Decellularized bone extracellular matrix in skeletal tissue engineering

2020 ◽  
Vol 48 (3) ◽  
pp. 755-764
Author(s):  
Benjamin B. Rothrauff ◽  
Rocky S. Tuan
Keyword(s):  
Tissue Engineering ◽  
Bone Regeneration ◽  
Tissue Regeneration ◽  
Animal Studies ◽  
Tumor Resection ◽  
Regenerative Capacity ◽  
Skeletal Tissue ◽  
Large Bone

Bone possesses an intrinsic regenerative capacity, which can be compromised by aging, disease, trauma, and iatrogenesis (e.g. tumor resection, pharmacological). At present, autografts and allografts are the principal biological treatments available to replace large bone segments, but both entail several limitations that reduce wider use and consistent success. The use of decellularized extracellular matrices (ECM), often derived from xenogeneic sources, has been shown to favorably influence the immune response to injury and promote site-appropriate tissue regeneration. Decellularized bone ECM (dbECM), utilized in several forms — whole organ, particles, hydrogels — has shown promise in both in vitro and in vivo animal studies to promote osteogenic differentiation of stem/progenitor cells and enhance bone regeneration. However, dbECM has yet to be investigated in clinical studies, which are needed to determine the relative efficacy of this emerging biomaterial as compared with established treatments. This mini-review highlights the recent exploration of dbECM as a biomaterial for skeletal tissue engineering and considers modifications on its future use to more consistently promote bone regeneration.

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