Regulation of Metabolic Pathways in Steroidogenic Cells by Ad4BP/SF-1

2018 ◽  
pp. 35-43
Author(s):  
Ken-ichirou Morohashi ◽  
Miki Inoue ◽  
Bing Li ◽  
Takashi Baba
Keyword(s):  
Metabolic Pathways ◽  
Steroidogenic Cells

High-resolution SEM and STEM of adrenocortical endothelium: Molecular resolution of membrane complexes

1993 ◽  
Vol 51 ◽  
pp. 610-611
Author(s):  
Robert P. Apkarian
Keyword(s):  
Fine Structure ◽  
Low Voltage ◽  
Uranyl Acetate ◽  
Correlative Microscopy ◽  
Coated Pits ◽  
Fine Grain ◽  
Structural Identity ◽  
Cellular Pathways ◽  
Steroidogenic Cells ◽  
Cr Film

A multitude of complex ultrastructural features are involved in endothelial cell (EC) gating and sorting of lipid through capillaries and into steroidogenic cells of the adrenal cortex. Correlative microscopy is necessary to distinguish the structural identity of features involved in specific cellular pathways. In addition to diaphragmed fenestrae that frequently appear in clusters, other 60-80 nm openings; plasmalemma vesicles (PV), channels and pockets fitted with diaphragms of the same dimension, coexist on the thin EC surface. Non-diaphragmed coated pits (CP) (100-120 nm) involved in receptor mediated endocytosis were also present on the EC membrane. The present study employed HRSEM of cryofractured and chromium coated specimens and low voltage HRSTEM of 80 nm thick LX-112 embedded sections stained with 2.0% uranyl acetate. Both preparations were imaged at 25 kV with a Topcon DS-130 FESEM equipped with in-lens stage and STEM detector.HRSEM images of the capillary lumen coated with a lnm continuous fine grain Cr film, provided the ability to scan many openings and resolve (SE-I contrast) the fine structure of diaphragm spokes and central densities (Fig. 1).

Accelerating Metabolic Pathways Simulation using GPUs

2010 ◽  
Author(s):  
Sohan Lal ◽  
Kolin Paul ◽  
James Gomes
Keyword(s):  
Metabolic Pathways

Mapping metabolic pathways in chemical property space paves the way for new leishmanicidals

Planta Medica ◽  
2016 ◽  
Vol 81 (S 01) ◽  
pp. S1-S381
Author(s):  
E Vikeved ◽  
R Buonfiglio ◽  
T Kogej ◽  
A Backlund
Keyword(s):  
Chemical Property ◽  
Metabolic Pathways ◽  
Property Space ◽  
The Way

METABOLISM OF PREGNENOLONE-3-SULPHATE IN THE PERFUSED DOG LIVER

1965 ◽  
Vol 49 (3) ◽  
pp. 427-435 ◽  
Author(s):  
K. D. Voigt ◽  
J. Tamm ◽  
U. Volkwein ◽  
H. Schedewie
Keyword(s):  
Metabolic Pathways ◽  
Dog Liver ◽  
Steroid Conjugates

ABSTRACT Pregnenolone-sulphate (400 mg) was perfused through isolated dog livers. The following steroids were isolated in the perfusate: pregnenolone, progesterone, dehydroepiandrosterone, androst-5-ene-diol and the two steroid conjugates, i. e. pregnenolone-sulphate and dehydroepiandrosterone-sulphate. Two »free« steroids and one steroid conjugate could not be characterized. A tentative scheme for the metabolic pathways of pregnenolone-sulphate is presented.

Synthesis and Breakdown of the Universal Precursors to Biological Metabolism Promoted by Ferrous Iron

2018 ◽  
Author(s):  
Kamila B. Muchowska ◽  
Sreejith Jayasree VARMA ◽  
Joseph Moran
Keyword(s):  
Amino Acids ◽  
Chemical Reaction ◽  
Metabolic Pathways ◽  
Ferrous Iron ◽  
Reaction Network ◽  
Tca Cycle ◽  
Chemical Reaction Network ◽  
Metabolic Precursors ◽  
Tca Cycle Intermediates

How core biological metabolism initiated and why it uses the intermediates, reactions and pathways that it does remains unclear. Life builds its molecules from CO<sub>2 </sub>and breaks them down to CO<sub>2 </sub>again through the intermediacy of just five metabolites that act as the hubs of biochemistry. Here, we describe a purely chemical reaction network promoted by Fe<sup>2+ </sup>in which aqueous pyruvate and glyoxylate, two products of abiotic CO<sub>2 </sub>reduction, build up nine of the eleven TCA cycle intermediates, including all five universal metabolic precursors. The intermediates simultaneously break down to CO<sub>2 </sub>in a life-like regime resembling biological anabolism and catabolism. Introduction of hydroxylamine and Fe<sup>0 </sup>produces four biological amino acids. The network significantly overlaps the TCA/rTCA and glyoxylate cycles and may represent a prebiotic precursor to these core metabolic pathways.

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