Immunological Methods for Detection and Identification of Yersinia pestis

2018 ◽  
pp. 251-257
Author(s):  
Yong Zhao
Keyword(s):  
Yersinia Pestis ◽  
Immunological Methods ◽  
Detection And Identification

scholarly journals Development of a DNA Microarray Method for Detection and Identification of All 15 Distinct O-Antigen Forms of Legionella pneumophila

2013 ◽  
Vol 79 (21) ◽  
pp. 6647-6654 ◽  
Author(s):  
Boyang Cao ◽  
Fangfang Yao ◽  
Xiangqian Liu ◽  
Lu Feng ◽  
Lei Wang
Keyword(s):  
Dna Microarray ◽  
Legionella Pneumophila ◽  
Detection Sensitivity ◽  
Immunological Methods ◽  
Clinical Samples ◽  
Content Type ◽  
O Antigen ◽  
Detection And Identification ◽  
Legionnaires Disease ◽  
Reference Strains

ABSTRACTLegionellais ubiquitous in many environments. At least 50 species and 70 serogroups of the Gram-negative bacterium have been identified. Of the 50 species, 20 are pathogenic, andLegionella pneumophilais responsible for the great majority (approximately 90%) of the Legionnaires' disease cases that occur. Furthermore, of the 15L. pneumophilaserogroups identified, O1 alone causes more than 84% of the Legionnaires' disease cases that occur worldwide. Rapid and reliable assays for the detection and identification ofL. pneumophilain water, environmental, and clinical samples are in great demand.L. pneumophilabacteria are traditionally identified by their O antigens by immunological methods. We have recently developed an O serogroup-specific DNA microarray for the detection of all 15 distinct O-antigen forms ofL. pneumophila, including serogroups O1 to O15. A total of 35 strains were used to verify the specificity of the microarray, including 15L. pneumophilaO-antigen standard reference strains and sevenL. pneumophilaclinical isolates as target strains, seven reference strains of other non-pneumophila Legionellaspecies as closely related strains, and six non-Legionellabacterial species as nonrelated strains. The detection sensitivity was 1 ng of genomic DNA or 0.4 CFU/ml in water samples with filter enrichment and plate culturing. This study demonstrated that the microarray allows specific, sensitive, and reproducible detection ofL. pneumophilaserogroups. To the best of our knowledge, this is the first report of a microarray serotyping method for all 15 distinct O-antigen forms ofL. pneumophila.

scholarly journals Detection and Identification of Virulence Factors in Yersinia pestis Using SELDI ProteinChip® System

BioTechniques ◽  
2001 ◽  
Vol 30 (2) ◽  
pp. 428-432 ◽  
Author(s):  
Vanitha Thulasiraman ◽  
Sandra L. McCutchen-Maloney ◽  
Vladimir L. Motin ◽  
Emilio Garcia
Keyword(s):  
Yersinia Pestis ◽  
Virulence Factors ◽  
Detection And Identification ◽  
Seldi Proteinchip

scholarly journals Laboratory Diagnosis of Cutaneous and Visceral Leishmaniasis: Current and Future Methods

2020 ◽  
Vol 8 (11) ◽  
pp. 1632
Author(s):  
Juliana Quero Reimão ◽  
Elizabeth Magiolo Coser ◽  
Monica Ran Lee ◽  
Adriano Cappellazzo Coelho
Keyword(s):  
Visceral Leishmaniasis ◽  
Microscopic Examination ◽  
Laboratory Diagnosis ◽  
Immunological Methods ◽  
Neglected Tropical Disease ◽  
Correct Identification ◽  
Specific Antibodies ◽  
New Methods ◽  
Detection And Identification

Leishmaniasis is a neglected tropical disease with two main clinical forms: cutaneous and visceral leishmaniasis. Diagnosis of leishmaniasis is still a challenge, concerning the detection and correct identification of the species of the parasite, mainly in endemic areas where the absence of appropriate resources is still a problem. Most accessible methods for diagnosis, particularly in these areas, do not include the identification of each one of more than 20 species responsible for the disease. Here, we summarize the main methods used for the detection and identification of leishmaniasis that can be performed by demonstration of the parasite in biological samples from the patient through microscopic examination, by in vitro culture or animal inoculation; by molecular methods through the detection of parasite DNA; or by immunological methods through the detection of parasite antigens that may be present in urine or through the detection of specific antibodies against the parasite. Potential new methods that can be applied for laboratory diagnosis of leishmaniasis are also discussed.

Biological Warfare Agents and their Detection and Monitoring Techniques (Review Paper)

2016 ◽  
Vol 66 (5) ◽  
pp. 445 ◽  
Author(s):  
Vijai Pal ◽  
M.K. Sharma ◽  
S.K. Sharma ◽  
A.K. Goel
Keyword(s):  
Social Impact ◽  
Cellular Fatty Acid ◽  
Immunological Methods ◽  
Biological Warfare ◽  
Detection And Identification ◽  
Biological Warfare Agents ◽  
Warfare Agents ◽  
Biological Event ◽  
Security Challenge ◽  
Identification Techniques

Recently, threat from biological warfare agents (BWAs) has emerged as the foremost national and global security challenge because of their simple and cheap production, easy dispersal, complicated detection, expensive protection and psychological, economical and social impact. Early detection and identification of BWAs during intentional biological event is essential to initiate corrective emergency responses for management of such incidents. Efforts are being made across the globe for development of state of the art technologies and systems for detection and identification of BWAs. However, till date there is no single system which can detect all the biothreat agents. In the present review, we describe the currently available techniques and systems for detection and identification of these agents. The basic identification techniques including biological culture, immunological methods, nucleic acid based detection, MALDI-TOF MS, cellular fatty acid profiling and flow cytometry based detection are presented. Detection of BWAs with biosensors, surface plasmon resonance, biological detectors, and stand-off detection systems is also summarized. However, despite of availability of several techniques and tools, no full proof system is available for detection/identification of all the BWAs.

Identification of hepatitis a virus in cell cultures by solid phase immune electron microscopy

1986 ◽  
Vol 44 ◽  
pp. 238-239
Author(s):  
C.D. Humphrey ◽  
T.L. Cromeans ◽  
E.H. Cook ◽  
D.W. Bradley
Keyword(s):  
Electron Microscopy ◽  
Liquid Phase ◽  
Cell Cultures ◽  
Rapid Detection ◽  
Hepatitis A ◽  
Hepatitis A Virus ◽  
Solid Phase ◽  
Immune Electron Microscopy ◽  
Detection And Identification

There is a variety of methods available for the rapid detection and identification of viruses by electron microscopy as described in several reviews. The predominant techniques are classified as direct electron microscopy (DEM), immune electron microscopy (IEM), liquid phase immune electron microscopy (LPIEM) and solid phase immune electron microscopy (SPIEM). Each technique has inherent strengths and weaknesses. However, in recent years, the most progress for identifying viruses has been realized by the utilization of SPIEM.

115: Detection and Identification of Bacterial DNA and HIV Gag in the Semen of HIV Infected Men

2004 ◽  
Vol 171 (4S) ◽  
pp. 30-30
Author(s):  
Robert C. Eyre ◽  
Ann A. Kiessling ◽  
Thomas E. Mullen ◽  
Rachel L. Kiessling
Keyword(s):  
Bacterial Dna ◽  
Detection And Identification
Sign in / Sign up

Export Citation Format

Share Document