Spectrophotometric Methods and Electronic Spin Resonance for Evaluation of Antioxidant Capacity of Food

2020 ◽  
pp. 53-75
Author(s):  
Mauricio Moncada-Basualto ◽  
Claudio Olea-Azar
Keyword(s):  
Antioxidant Capacity ◽  
Spectrophotometric Methods ◽  
Electronic Spin ◽  
Spin Resonance

scholarly journals The Early Effect of Carotid Artery Stenting on Antioxidant Capacity and Oxidative Stress in Patients with Carotid Artery Stenosis

2016 ◽  
Vol 2016 ◽  
pp. 1-8 ◽  
Author(s):  
Slawomir Michalak ◽  
Wojciech Ambrosius ◽  
Ewa Wysocka ◽  
Mieczyslaw Dziarmaga ◽  
Robert Juszkat ◽  
...  
Keyword(s):  
Carotid Artery ◽  
Antioxidant Capacity ◽  
Carotid Artery Stenosis ◽  
Carotid Artery Stenting ◽  
Antioxidant Defense ◽  
Artery Stenosis ◽  
Sulfhydryl Groups ◽  
Early Effect ◽  
Spectrophotometric Methods ◽  
And Oxidative Stress

The treatment of carotid artery stenosis is associated with the risk of complications, which may include stroke after carotid artery stenting (CAS) and myocardial infarction after carotid endarterectomy (CEA). The imbalance between prooxidative mechanisms and antioxidant capacity creates a milieu of factors, which may increase the risk of complications after endovascular procedures. We have examined 43 consecutive patients with carotid artery stenosis. Sera were analyzed for the activity of paraoxonase (PON) and arylesterase (ARE), sulfhydryl groups (SG), malondialdehyde (MDA), and conjugated dienes (CD) concentrations by means of spectrophotometric methods before and next day after CAS. We have found lowered PON (P=0.0032), increase in ARE activity (P=0.0058), and decrease in sulfhydryl groups concentration (P=0.0267). No effect on absolute MDA and CD concentrations was observed. The degree of carotid artery stenosis correlated negatively with PON/ARE ratio after CAS (rS= −0.507,P=0.0268). To conclude, CAS influences both enzymatic (differently, PON and ARE activity) and nonenzymatic antioxidant defense. Females are more susceptible to lipid peroxidation after CAS. PON/ARE ratio after CAS correlated with the degree of carotid artery stenosis. The changes (deltas) in ARE activity, SG, and MDA concentrations correlated with the severity of neurological deficit and disability.

Electronic spin resonance spectra of SiH3 radicals trapped in the low temperature matrix of nonmagnetic isotopes of xenon

1989 ◽  
Vol 90 (6) ◽  
pp. 2992-2994 ◽  
Author(s):  
K. Nakamura ◽  
M. Okamoto ◽  
T. Takayanagi ◽  
T. Kawachi ◽  
K. Shimokoshi ◽  
...  
Keyword(s):  
Low Temperature ◽  
Electronic Spin ◽  
Temperature Matrix ◽  
Spin Resonance

Plasmatic antioxidant capacity due to ascorbate using TEMPO scavenging and electron spin resonance

2005 ◽  
Vol 359 (1-2) ◽  
pp. 78-88 ◽  
Author(s):  
Lidia L. Piehl ◽  
Graciela B. Facorro ◽  
Mónica G. Huarte ◽  
Martín F. Desimone ◽  
Guillermo J. Copello ◽  
...  
Keyword(s):  
Electron Spin Resonance ◽  
Antioxidant Capacity ◽  
Electron Spin ◽  
Spin Resonance

scholarly journals Determination of in Vitro Antioxidant Enzyme Capacity and Oxidative Stress Levels in Mazı Meşesi (Quercus infectoria)

2021 ◽  
Vol 9 (4) ◽  
pp. 814-817
Author(s):  
İlter Demirhan ◽  
Büşra Çitil ◽  
Mehmet Özyurt ◽  
Meltem Güngör ◽  
Erkan Öner ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Superoxide Dismutase ◽  
Antioxidant Capacity ◽  
Antioxidant Enzyme ◽  
Antioxidant Enzyme Activities ◽  
Spectrophotometric Methods ◽  
Significant Difference ◽  
Quercus Infectoria

South East Anatolia Region has a large genetic plant diversity due to its physical and different climatic charesteristics. These plants are potential sources of antioxidants that prevent oxidative stress caused by oxygen and photons. In recent years, it has become important to study the antioxidant capacity of many molecules found naturally in foods and biological systems. The reason for this is that it is believed that when the consumption of food rich in antioxidants is increased, the risk of developing different degenerative diseases will be reduced. In this study, it was aimed to measure the antoxidant capacity of Quercus infectoria, G.olivier gal seeds grown in Southeastern Anatolia. Q. infectoria gal seeds from Sanlıurfa province were used in our study. Q. infectoria gal seeds were extracted with water, ethanol and methanol and then antioxidant enzyme activities (catalase and superoxide dismutase) and malondialdehyde levels, which are indicators of oxidative stress were determined by spectrophotometric methods. It was found that the antioxidant capacity (catalase and superoxide dismutase activities) of extracts obtained from ethanol and methanol were higher and their malondialdehyde levels were statistically lower than those obtained from water. However, it was determined that there was no statistically significant difference between the antioxidant capacity and malondialdehyde levels of the extracts obtained from methanol compared to the extracts obtained from ethanol. It has been concluded that Q. infectoria gal seed has a effective antioxidant effect. In addition, it was observed that extracts obtained from ethanol and methanol have higher antioxidant capacity than extracts obtained from water.

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