Biotechnological Application of Meta-topolins as Highly Active Aromatic Cytokinins in Micropropagation of Medicinal Plants

Objective: The present study was aimed to evaluate the antibacterial effect of Himalayan medicinal plants on several human pathogens.Methods: The leaves of Mesua ferrea, Cannabis sativa, and Urtica dioica, bark of Juglans regia, Glycyrrhiza glabra, fruit of Terminalia chebula, and seed of Piper nigrum were dried and powdered before methanolic extraction (ME). Antibacterial assay of MEs was performed against Bacillus subtilis, Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, and Klebsiella pneumoniae using agar well diffusion method. The minimum inhibitory concentrations of these extracts were assessed using microdilution method. The phytochemical analysis of these extracts was carried out to know the bioactive compounds and was fractionated with various solvents such as chloroform, butanol, ethyl acetate, and hexane to isolate the bioactive compound.Results: The ME of C. sativa was found highly active, followed by T. chebula, J. regia, G. glabra, and M. ferrea against these pathogens. While the extracts of U. dioica and P. nigrum found less effective. MIC values of J. regia extract were found much more significant (0.219 g/ml). The antibacterial assay of fractions revealed that the chloroform fraction was highly active against all the pathogens than those of ethyl acetate, butanol, and hexane.Conclusion: It was concluded from the present study that methanol was a good solvent for isolating compounds from medicinal plants and also favors the traditional uses of medicinal plants as antibacterial agents and as a potential source for the new drug discovery. It could also be possible that the compound responsible for antibacterial activity must be present in chloroform fraction of J. regia.

Download Full-text

ANTIMICROBIAL ACTIVITY OF SOME FOLK MEDICINAL PLANTS USED IN RAJASTHAN AGAINST SELECTED PATHOGENIC MICROORGANISMS

International Journal of Current Pharmaceutical Research ◽

10.22159/ijcpr.2017.v9i3.19595 ◽

2017 ◽

Vol 9 (3) ◽

pp. 84

Author(s):

Maya Verma ◽

Ashwani Kumar

Keyword(s):

Antifungal Activity ◽

Medicinal Plants ◽

Leaf Extract ◽

Diffusion Method ◽

Bacterial Strains ◽

Leaf Extracts ◽

Phyllanthus Niruri ◽

E Coli ◽

Methanol Extracts ◽

Highly Active

Objective: To investigate the antibacterial efficacy of methanol extracts of leaves and roots of B. diffusa, Eclipta alba, Phyllanthus niruri and Ricinus communis.Methods: The antimicrobial efficacy of methanol extracts of some medicinal plants was evaluated by agar well diffusion method against selected pathogenic bacterial strains. Gram+ve strains (S. aureus, B. subtilis) were tested and Gram-ve strains tested were (E. coli, S. typhii and K. pneumoniae). Antifungal activity against was tested.Results: B. diffusa and P. niruri leaf extract showed highest antibacterial activity against S. aureus and S. typhii. Leaf extract of P. niruri and R. communis showed highest antifungal activity against A. niger and C. albicans respectivelyConclusion: The methanolic leaf extracts of B. diffusa and P. niruri were highly active against S. aureus and S. typhii.

Download Full-text

Effect of pomegranate fruit supplementation on performance and various markers in athletes and active subjects: a systematic review

International Journal for Vitamin and Nutrition Research ◽

10.1024/0300-9831/a000601 ◽

2019 ◽

pp. 1-15

Author(s):

Alicja Urbaniak ◽

Anna Skarpańska-Stejnborn

Keyword(s):

Vessel Diameter ◽

Whole Body ◽

Double Blind Study ◽

Double Blind ◽

Physically Active ◽

Highly Active ◽

Body Strength ◽

Rate Of Increase ◽

Study Designs ◽

Pomegranate Fruit

Abstract. The aim of the study was to review recent findings on the use of POM supplements in athletes of various disciplines and physically active participants. Eleven articles published between 2010 and 2018 were included, where the total number of investigated subjects was 176. Male participants constituted the majority of the group (n = 155), as compared to females (n = 21). 45% of research described was conducted on athletes, whereas the remaining studies were based on highly active participants. Randomised, crossover, double-blind study designs constituted the majority of the experimental designs used. POM supplementation varied in terms of form (pills/juice), dosage (50 ml–500 ml) and time of intervention (7 days–2 months) between studies. Among the reviewed articles, POM supplementation had an effect on the improvement of the following: whole body strength; feeling of vitality; acute and delayed muscle fatigue and soreness; increase in vessel diameter; blood flow and serum level of TAC; reduction in the rate of increase for HR, SBP, CK and LDH; support in the recovery of post-training CK, LDH, CRP and ASAT to their baseline levels; reduction of MMP2, MMP9, hsCRP and MDA; and increased activity of antioxidant enzymes (glutathione peroxidase and superoxide dismutase). In the majority of reviewed articles POM supplementation had a positive effect on a variety of parameters studied and the authors recommended it as a supplement for athletes and physically active bodies.

Download Full-text