Correlation and Autofluorescence Microscopy in Forensics Medicine: Time of Death Detection Using Polycrystalline Cerebrospinal Fluid Films

2021 ◽  
Author(s):  
M.S. Harazdyuk ◽  
V.T. Bachinsky ◽  
O.Ya. Wanchulyak ◽  
A. G. Ushenko ◽  
Yu. A. Ushenko ◽  
...  
Keyword(s):  
Cerebrospinal Fluid ◽  
Time Of Death ◽  
Fluid Films

METACHROMATIC LEUKODYSTROPHY: CLINICAL, HISTOCHEMICAL, AND CEREBROSPINAL FLUID ABNORMALITIES

PEDIATRICS ◽  
1962 ◽  
Vol 30 (4) ◽  
pp. 629-638
Author(s):  
Richard J. Allen ◽  
James J. McCusker ◽  
Wallace W. Tourtellotte
Keyword(s):  
Central Nervous System ◽  
Cerebrospinal Fluid ◽  
Nervous System ◽  
Metachromatic Leukodystrophy ◽  
Lipid Analysis ◽  
Time Of Death ◽  
System Degeneration ◽  
Cherry Red Spot ◽  
Histochemical Changes ◽  
Acid Esters

A child has been reported with histochemically proven metachromatic leukodystrophy. Central nervous system degeneration began at the age of 20 months and continued to the time of death at the age of 49 months. Prior to death a "cherry red spot" became evident in the retina and is the only known instance of this finding. This adds to the evidence that metachromatic leukodystrophy is one of the neurolipidoses, but the metabolic defect apparently affects primarily the cerebroside sulfuric acid esters. Repeated analyses of cerebrospinal fluid demonstrated an elevated total protein and a "midzone" gold curve. Lipid analysis of serum and cerebrospinal fluid demonstrated findings which are discussed in detail and correlated to the histochemical changes. In addition, comparisons are made with the cerebrospinal fluids in Tay-Sach's and Niemann-Pick's disease. This too revealed important differences between the various neurolipidoses.

Tau protein in cerebrospinal fluid: a novel biomarker of the time of death?

2021 ◽  
Author(s):  
Pierre-Antoine Peyron ◽  
Christophe Hirtz ◽  
Eric Baccino ◽  
Nelly Ginestet ◽  
Laurent Tiers ◽  
...  
Keyword(s):  
Cerebrospinal Fluid ◽  
Tau Protein ◽  
Time Of Death

scholarly journals Estimation of the time of death by measuring the variation of lateral cerebral ventricle volume and cerebrospinal fluid radiodensity using postmortem computed tomography

2021 ◽  
Author(s):  
Fabio De-Giorgio ◽  
Gabriele Ciasca ◽  
Gennaro Fecondo ◽  
Alberto Mazzini ◽  
Marco De Spirito ◽  
...  
Keyword(s):  
Cerebrospinal Fluid ◽  
Time Range ◽  
Postmortem Computed Tomography ◽  
Time Of Death ◽  
Cerebral Ventricles ◽  
3D Segmentation ◽  
Postmortem Ct ◽  
Ventricle Volume ◽  
Lateral Cerebral Ventricle ◽  
Over Time

Abstract Using postmortem CT (PMCT), changes in the volume of the lateral cerebral ventricles (LCVs) and modifications of the radiodensity of cerebrospinal fluid (CSF) have been examined to identify a possible relationship between these changes and the time of death. Subsequent periodical CT scans termed “sequential scans” for ten corpses at known time of death were obtained, and a 3D segmentation of the entire LCV was carried out to measure its volume and radiodensity over time from ~ 5.5- h up to 273-h postmortem. A linear decrease of the LCV volume for all the cases was observed in the investigated time range, together with an overall logarithmic increase of radiodensity. Although a larger sampling should be performed to improve the result reliability, our finding suggests that the postmortem variation of CSF radiodensity can be a potentially useful tool in determining postmortem interval, a finding that is worthy of further investigation.

scholarly journals POST-MORTEM INTERVAL ESTIMATION USING POLYCRYSTALLINE CEREBROSPINAL FLUID FILMS TWO-DIMENSIONAL CARTOGRAPHY OF MUTUAL LASER POLARIZATION DISTRIBUTION LEVELS

2018 ◽  
Vol 6 ◽  
pp. 892-897
Author(s):  
Marta Garazdiuk ◽  
Oleh Wanchuliak ◽  
Oleksandr Pavlyukovich ◽  
Natalia Pavlyukovich ◽  
Oleksandr Garazdiuk
Keyword(s):  
Cerebrospinal Fluid ◽  
Large Scale ◽  
Interval Estimation ◽  
Diagnostic Methods ◽  
Two Dimensional ◽  
Post Mortem ◽  
Polycrystalline Films ◽  
Fluid Films ◽  
Microscopic Images ◽  
Post Mortem Interval

Post-mortem interval estimation is one of the most important issues in forensic practice. Optical diagnostic methods of biological tissue structure assessment are perspective in this area.The objects of investigation are polycrystalline films of cerebrospinal fluid, taken from 64 corpses with accurately known times of death and 15 healthy volunteers.The method of two-dimensional stokes-polarimetric mapping of distributions of a complex degree of mutual polarization with spatial-frequency filtration of microscopic images of cerebrospinal fluid films in the time monitoring of post-mortem changes in optical manifestations of polycrystalline networks has been tested in order to estimate the post-mortem interval. The most sensitive post-mortem changes in the optical manifestations of polycrystalline cerebrospinal fluid networks are revealed - statistical moments of the third and fourth orders that characterize the asymmetry and the excess (severity of the peak) of the distributions of values of the complex degree of mutual polarization of large-scale components of cerebrospinal fluid polycrystalline films microscopic images.An interval of 48 hours with the accuracy of the post-mortem interval estimation in ± 30 minutes were established by the method of two-dimensional mapping of distributions of the values of a complex degree of mutual polarization of large-scale component of cerebrospinal fluid films microscopic images.

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