Measurement of nutrient spiralling during a period of continuous surface flow in a Mediterranean temporary stream (Arroyo de La Montesina, Spain)

SummaryThe mode of F Xa inhibition was investigated on a thromboresistant surface with end-point attached partially depoly-merized heparin of an approximate molecular weight of 8000. Affinity chromatography revealed that one fourth of the heparin used in surface coating had high affinity for antithrombin III (AT). The heparin surface adsorbed AT from both human plasma and solutions of purified AT. By increasing the ionic strength in the AT solution the existence of high and low affinity sites could be shown. The uptake of AT was measured and the density of available high and low affinity sites was found to be in the range of 5 HTid 11 pic.omoles/cmf, respectively Thus the estimated density of biologically active high and low ailmity heparm respectively would be 40 and 90 ng/cm2 The heparin coating did not take up or exert F Xa inhibition by itself. With AT adsorbed on both high and low affinity heparin the surface had the capacity to inhibit several consecutive aliquots of F Xa exposed to the surface. When mainly high affinity sites were saturated with AT the inhibition capacity was considerably lower. Tt was demonstrated that the density of AT on both high and low affinity heparin determines the F Xa inhibition capacity whereas the amount of AT on high affinity sites limits the rate of the reaction. This implies that during the inhibition of F Xa there is a continuous surface-diffusion of AT from sites of a lower class to the high affinity sites where the F Xa/AT complex is formed and leaves the surface. The ability of the immobilized heparin to catalyze inhibition of F Xa is likely to be an important component for the thromboresistant properties of a heparin coating with non-compromized AT binding sequences.

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IDENTIFYING THE MOST SUITABLE AREAS FOR SURFACE FLOW WETLAND CONSTRUCTION IN LITHUANIA

Proceedings of the 7th International Scientific Conference Rural Development 2015 ◽

10.15544/rd.2015.060 ◽

2015 ◽

Author(s):

Antanas DUMBRAUSKAS ◽

Nijolė BASTIENĖ ◽

Petras PUNYS

Keyword(s):

Constructed Wetlands ◽

Ecological Status ◽

Surface Flow ◽

Gis Analysis ◽

Proximity Analysis ◽

Wetland Construction ◽

The Mean ◽

Two Phases ◽

Terrain Features ◽

Surface Flow Constructed Wetlands

GIS-based approach to find the suitable sites for surface flow constructed wetlands was employed for the Lithuanian river basins with low ecological status. According to the nature of the analysed criteria the flowchart consists of two phases. Criteria used include hydrographical network, soil properties, terrain features, land use, etc. Some of them have strictly defined values (constraints), and other ranges within certain limits (factors). Limited criteria were analysed using rejection principle and influencing factors using proximity analysis and overlay methods. Selecting the potential sites using standard GIS analysis tools there was estimated about 3286 sites for possible wetlands with the mean area of inflow basin about 4 km2 in the basins of water bodies at risk.

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