A barley cDNA clone encoding a type III chlorophyll a/b-binding polypeptide of the light-harvesting complex II

1992 ◽  
Vol 19 (4) ◽  
pp. 699-703 ◽  
Author(s):  
Jakob Brandt ◽  
Vibeke Skovgaard Nielsen ◽  
Hans Thordal-Christensen ◽  
David John Simpson ◽  
Jens Sigurd Okkers
1995 ◽  
Vol 69 (6) ◽  
pp. 2670-2678 ◽  
Author(s):  
E.J. Peterman ◽  
F.M. Dukker ◽  
R. van Grondelle ◽  
H. van Amerongen

1999 ◽  
Vol 54 (9-10) ◽  
pp. 746-753 ◽  
Author(s):  
Éva Sárvári ◽  
Ferenc Fodor ◽  
Edit Cseh ◽  
Anita Varga ◽  
Gyula Záray ◽  
...  

Hydroponically cultured cucumber plants supplied with 4 μᴍ Fe chelated either with EDTA or citrate and treated with Cd (10 μᴍ) and Pb (10, 50 μᴍ) from their one- or fourleaf stage were grown up to five-week-old age. The decrease in the chlorophyll content was the most pronounced in plants treated with Cd from a younger age, and in the case of Fecitrate. The chlorophyll a/b ratio of Cd stressed plants was also significantly lowered. In later treated plants the accumulation of chlorophyll was inhibited and the chlorophyll a/b ratio decreased only in the vigorously growing young leaves. Pb treatment had only a slight effect on both parameters. The changes in the chlorophyll-protein pattern of thylakoids were strongly related to their chlorophyll content but the response of each complex was different. Cd reduced the amount of chlorophyll containing complexes in the order of photosystem I > light-harvesting complex II > photosystem Il-core, while light-harvesting complex II appeared somewhat more sensitive than photosystem I in Pb treated plants. In accordance, a decline or blue shift of the long wavelength fluorescence emission band of chloroplasts was observed referring to disturbances also in photosystem I antenna assembly. The accumulation of chlorophyll and chlorophyll-proteins did not show close relationship to the heavy metal content of leaves which was the highest in the first of the intensively expanding leaves in the time of the treatment. The extraordinary sensitivity of photosystem I, and the relative stability of photosystem II under Cd treatment were similar to the case found in iron deficient plants. However, the pattern of chlorophyll content of leaf storeys of Cd treated plants rather followed the changes in their Mn content


2016 ◽  
Vol 8 (1) ◽  
pp. 257-263 ◽  
Author(s):  
Parveen Akhtar ◽  
Cheng Zhang ◽  
Thanh Nhut Do ◽  
Győző Garab ◽  
Petar H. Lambrev ◽  
...  

2019 ◽  
Vol 476 (20) ◽  
pp. 2981-3018 ◽  
Author(s):  
Petar H. Lambrev ◽  
Parveen Akhtar

Abstract The light reactions of photosynthesis are hosted and regulated by the chloroplast thylakoid membrane (TM) — the central structural component of the photosynthetic apparatus of plants and algae. The two-dimensional and three-dimensional arrangement of the lipid–protein assemblies, aka macroorganisation, and its dynamic responses to the fluctuating physiological environment, aka flexibility, are the subject of this review. An emphasis is given on the information obtainable by spectroscopic approaches, especially circular dichroism (CD). We briefly summarise the current knowledge of the composition and three-dimensional architecture of the granal TMs in plants and the supramolecular organisation of Photosystem II and light-harvesting complex II therein. We next acquaint the non-specialist reader with the fundamentals of CD spectroscopy, recent advances such as anisotropic CD, and applications for studying the structure and macroorganisation of photosynthetic complexes and membranes. Special attention is given to the structural and functional flexibility of light-harvesting complex II in vitro as revealed by CD and fluorescence spectroscopy. We give an account of the dynamic changes in membrane macroorganisation associated with the light-adaptation of the photosynthetic apparatus and the regulation of the excitation energy flow by state transitions and non-photochemical quenching.


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