The influence of culture conditions on anther culture response of commercial varieties of Solanum tuberosum L.

1992 ◽  
Vol 30 (3) ◽  
pp. 211-219 ◽  
Author(s):  
Taina Tiainen
Euphytica ◽  
1973 ◽  
Vol 22 (2) ◽  
pp. 317-323 ◽  
Author(s):  
J. M. Dunwell ◽  
N. Sunderland

Uniciencia ◽  
2017 ◽  
Vol 31 (2) ◽  
pp. 121
Author(s):  
José Antonio García-García ◽  
José Bernal Azofeifa-Bolaños

In order to understand the performance of Solanum tuberosum L. “Floresta” in producing microtubers, the effect of four concentrations of boric acid (6.2 mg l-1, 7.75 mg l-1, 9.3 mg l-1, and 10.85 mg l-1) under two conditions—1) 8 hour dark and 2) 16 hour light photoperiod and 8 hour dark—was evaluated under in vitro culture conditions. Full-strength Murashige and Skoog medium was used as a control. The data analysis for the number and biomass of microtubers were significant for the condition (p ≤ 0.05). Concerning the concentration of boric acid and the interaction between factors, a significant difference was obtained for biomass (p ≤ 0.05). In dark conditions, a greater number of microtubers was obtained, although the biomass was lower compared to the light conditions where the biomass was higher, and the number of microtubers was lower. A concentration of 9.3 mg l-1 of boric acid in both types of conditions was the best treatment to produce a greater number and more biomass of microtubers. The differences identified in this work with regards to the number and biomass of microtubers were probably the combined result of genotype and specific culture conditions. Even considering this, the use of lighting conditions is proposed to induce larger and greener microtubers.


2016 ◽  
Vol 38 (2) ◽  
Author(s):  
Yong Rhee ◽  
KyungRan Hwang ◽  
Seungchan Cho ◽  
Minji Lee ◽  
Eui-Joon Kil ◽  
...  

Author(s):  
Andreea NISTOR ◽  
Nicoleta CHIRU ◽  
Mihaela CIOLOCA ◽  
Monica POPA

Five genotypes of potato (Solanum tuberosum L.) were induced to form microtubers under two in vitro culture conditions (continuos darkness and light). Cultures maintained under continuos darkness (with a temperatue of 170C) had a higher yield with a greater number of microtuber (1.394/plantlet) than those maintained under long days (16 h of light/day) combined with temperature of 200C. In the last case the microtubers were higher, with with the highest average weight of 0.602 g. Three phytohormones it was used: coumarine, benzylaminopurine and salicylic acid. Regarding the average number of microtubers obtained when it was used coumarine and salicylic acid, the highest average number of microtubers was recorded (1,135 microtubers/plantlet). The study had been performed also on two fractions of the of sugar quantity (80 and 140 g/l), but they had less influence compared to the varieties.


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