The use of agarose bead culture for the regeneration of single cell-derived colonies from protoplasts isolated from suspension cultures of Humulus lupulus

1987 ◽  
Vol 8 (1) ◽  
pp. 17-25 ◽  
Author(s):  
Judith M. Furze ◽  
Michael J. C. Rhodes ◽  
Richard J. Robins
Keyword(s):  
Single Cell ◽  
Suspension Cultures ◽  
Humulus Lupulus ◽  
Agarose Bead

scholarly journals Single-cell microscopy of suspension cultures using a microfluidics-assisted cell screening platform

2017 ◽  
Vol 13 (1) ◽  
pp. 170-194 ◽  
Author(s):  
Burak Okumus ◽  
Charles J Baker ◽  
Juan Carlos Arias-Castro ◽  
Ghee Chuan Lai ◽  
Emanuele Leoncini ◽  
...  
Keyword(s):  
Single Cell ◽  
Suspension Cultures ◽  
Screening Platform ◽  
Cell Microscopy

p-Hydroxynonanophenone in cell suspension cultures of a Humulus lupulus cultivar

1991 ◽  
Vol 30 (2) ◽  
pp. 495-496 ◽  
Author(s):  
Amitabh Chandra ◽  
Carlos R. Langezaal ◽  
Johannes J.C. Scheffer
Keyword(s):  
Cell Suspension ◽  
Cell Suspension Cultures ◽  
Suspension Cultures ◽  
Humulus Lupulus

On the generality of methods to obtain single-cell plant suspension cultures

1978 ◽  
Vol 56 (20) ◽  
pp. 2521-2527 ◽  
Author(s):  
D. J. Kubek ◽  
M. L. Shuler
Keyword(s):  
Single Cell ◽  
Cell Cultures ◽  
Mass Number ◽  
Cell Mass ◽  
Continuous Cultivation ◽  
Suspension Cultures ◽  
Media Composition ◽  
Glycine Max L ◽  
The Media ◽  
Biochemical State

Several techniques employing mechanical, chemical, or enzymatic methods have been suggested for the production of essentially single-cell plant suspension cultures. If single-cell cultures can be obtained, the effects of the media composition on growth can be unambiguously determined. Additionally, such cultures would be amenable to optical and electronic methods for rapidly determining cell mass, number, and volume and could easily be used in experiments on continuous cultivation. Most methods to produce single-cell cultures have been applied to only one or two species. In this paper, these techniques are compared when extended to cultures of Paul's Scarlet Rose (Rosa sp.) cells and soybean cells (Glycine max L.). It is concluded that no technique will generally give sustained disaggregation without affecting the apparent biochemical state of the culture.

Sign in / Sign up

Export Citation Format

Share Document