Protoplast isolation, culture, and fusion protocols for kenaf (Hibiscus cannabinus)

1996 ◽  
Vol 44 (3) ◽  
pp. 201-210 ◽  
Author(s):  
Nancy A. Reichert ◽  
Donglong Liu
Keyword(s):  
Protoplast Isolation ◽  
Hibiscus Cannabinus

scholarly journals PROTOPLAST ISOLATION AND CULTURE OF KENAF (HIBISCUS CANNABINUS L.)

HortScience ◽  
1994 ◽  
Vol 29 (7) ◽  
pp. 729e-729
Author(s):  
Donglong Liu ◽  
Nancy A. Reichert
Keyword(s):  
Cell Division ◽  
Liquid Medium ◽  
Hydrolytic Enzymes ◽  
Leaf Tissue ◽  
Protoplast Isolation ◽  
Hibiscus Cannabinus ◽  
Cultivar Differences ◽  
Fusion Frequency ◽  
Protoplast Yield ◽  
Acceptable Range

Protoplast isolation and culture protocols were developed for leaf tissue from 6 kenaf cultivars [Everglades 41 (E41), E71, Guatemala 4 (G4), G45, G51, and Tainung 1]. For protoplast isolation, the best combination of hydrolytic enzymes was cellulysin (1% w/v; Calbiochem) plus macerase (0.5% w/v; Calbiochem), with a 24 hour digestion at 30°C in the dark. Yields reached 7.2 (10)6 protoplasts/g leaf tissue. Protoplast viabilities ranged from 65% to 96%. Minor cultivar differences were observed related to protoplast yield, but all viability estimates were in an acceptable range. Greatest cell division frequencies and plating efficiencies were obtained when protoplasts were initially cultured in liquid medium at a density of 1.0 (10)5 protoplasts/ml. Electrofusion protocols were developed for kenaf protoplasts testing the range from 1200 to 3000 V/cm. A fusion voltage of 2000 V/cm yielded the highest fusion frequency and retained viability above 80%.

Protoplast Isolation and Fusion Induced by PEG with Leaves and Roots of Tea Plant (Camellia sinensis L. O. Kuntze)

2018 ◽  
Vol 44 (3) ◽  
pp. 463 ◽  
Author(s):  
Zhang PENG ◽  
Hua-Rong TONG ◽  
Guo-Lu LIANG ◽  
Yi-Qi SHI ◽  
Lian-Yu YUAN
Keyword(s):  
Camellia Sinensis ◽  
Protoplast Isolation ◽  
Tea Plant ◽  
Leaves And Roots

Effect of Plant Spacing and Time of Planting on Fiber Yield of Kenaf, Hibiscus Cannabinus L. 1

1946 ◽  
Vol 38 (1) ◽  
pp. 46-59 ◽  
Author(s):  
Julian C. Crane ◽  
Julían B. Acuña ◽  
Raul E. Alonso
Keyword(s):  
Hibiscus Cannabinus ◽  
Plant Spacing ◽  
Fiber Yield

Kenaf (Hibiscus cannabinus L.) based substrates for the production of compact plants

2005 ◽  
Vol 21 (2) ◽  
pp. 223-227 ◽  
Author(s):  
A. Tsakonas ◽  
V. Stergiou ◽  
M. Polissiou ◽  
K. Akoumianakis ◽  
H.C. Passam
Keyword(s):  
Hibiscus Cannabinus

Callus induction, suspension culture and protoplast isolation in Camellia oleifera

2021 ◽  
Vol 286 ◽  
pp. 110193
Author(s):  
Su-Fang Li ◽  
Tian-Wen Ye ◽  
Xin Xu ◽  
De-Yi Yuan ◽  
Shi-Xin Xiao
Keyword(s):  
Suspension Culture ◽  
Callus Induction ◽  
Protoplast Isolation ◽  
Camellia Oleifera

scholarly journals Thermal degradation of kenaf (Hibiscus cannabinus L.): Impact of torrefaction on pyrolysis kinetics and thermal behavior

2021 ◽  
Vol 7 ◽  
pp. 951-959
Author(s):  
Byoung-Hwa Lee ◽  
Tae-Yong Jeong ◽  
Viet Thieu Trinh ◽  
Chung-Hwan Jeon
Keyword(s):  
Thermal Degradation ◽  
Thermal Behavior ◽  
Hibiscus Cannabinus ◽  
Pyrolysis Kinetics

scholarly journals Medium Density Fibreboard Made from Kenaf (Hibiscus cannabinus L.) Stem: Effect of Thermo-mechanical Refining and Resin Content

BioResources ◽  
2014 ◽  
Vol 9 (2) ◽  
Author(s):  
Majid Dehghan Nayeri ◽  
Paridah Md Tahir ◽  
Mohammad Jawaid ◽  
Zaidon Ashaari ◽  
Luqman Chuah Abdullah ◽  
...  
Keyword(s):  
Resin Content ◽  
Hibiscus Cannabinus ◽  
Medium Density ◽  
Medium Density Fibreboard ◽  
Mechanical Refining

Chemical and Morphological Characteristics of Malaysian Cultivated Kenaf (Hibiscus cannabinus) Fiber

2006 ◽  
Vol 45 (1) ◽  
pp. 131-134 ◽  
Author(s):  
Alireza Ashori ◽  
Jalaluddin Harun ◽  
Warwick D. Raverty ◽  
Mohd. Nor Mohd. Yusoff
Keyword(s):  
Morphological Characteristics ◽  
Hibiscus Cannabinus

Eco-Friendly of Hibiscu cannabinus L. Fibers Dyeing with Natural Dye

2013 ◽  
Vol 781-784 ◽  
pp. 674-677
Author(s):  
Sudaporn Tangkawanit
Keyword(s):  
Natural Dye ◽  
Hibiscus Cannabinus ◽  
Intensive Study ◽  
Light Fastness ◽  
Color Strength ◽  
Caesalpinia Sappan ◽  
Washing Fastness ◽  
Fastness Property ◽  
Level 3

This research was an intensive study on development of Hibiscus cannabinus L. fibers dyeing with natural dye. The result reflected that color shade and color strength value results were different depend on type of mordanted. The Hibiscus cannabinus L. washing with detergent dyeing with Caesalpinia sappan L. and various mordented showed that light fastness was in level 3-4, when CuSO4 and FeSO4 mordanted light fastness result was in level 4-5, mordants as lime, CuSO4 and FeSO4 were added washing fastness property was in level 5.

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