Determination of phosphoglucomutase (PGM1), acid phosphatase (ACP), and esterase D (ESD) in human bloodstains by hybrid isoelectric focusing (HIEF)

1989 ◽  
Vol 102 (4) ◽  
Author(s):  
I. Mu�oz-Bar�s ◽  
M.V. Lareu ◽  
I. L�pez-Rodriguez ◽  
M.S. Rodriguez-Calvo ◽  
A. Carracedo
Keyword(s):  
Acid Phosphatase ◽  
Isoelectric Focusing ◽  
Hybrid Isoelectric Focusing

Determination of Orosomucoid (ORM) Phenotypes by Hybrid Isoelectric Focusing

1988 ◽  
Vol 21 (4) ◽  
pp. 209-212 ◽  
Author(s):  
I López-Rodriguez ◽  
Md. Montiel ◽  
A. Carracedo ◽  
Ms. Rodriguez-Calvo ◽  
Mv. Lareu ◽  
...  
Keyword(s):  
Isoelectric Focusing ◽  
Hybrid Isoelectric Focusing

Comparison of human and murine isolates of Schistosoma mansoni from Richard-Toll, Senegal, by isoelectric focusing

1997 ◽  
Vol 71 (2) ◽  
pp. 175-181 ◽  
Author(s):  
M. Sène ◽  
P. Brémond ◽  
J.P. Hervé ◽  
V.R. Southgate ◽  
B. Sellin ◽  
...  
Keyword(s):  
Genetic Variation ◽  
Acid Phosphatase ◽  
Lactate Dehydrogenase ◽  
Schistosoma Mansoni ◽  
Isoelectric Focusing ◽  
Glucose Phosphate Isomerase ◽  
Glucose Phosphate ◽  
Enzyme Systems ◽  
Glucose 6 Phosphate Dehydrogenase ◽  
Arvicanthis Niloticus

AbstractStudies on human and murine isolates of Schistosoma mansoni, from Richard-Toll, Senegal, were carried out by isoelectric focusing in polyacrylamide gels. Seven enzyme systems; lactate dehydrogenase (LDH), malate dehydrogenase (MDH), glucose-6-phosphate dehydrogenase (G6PD), acid phosphatase (AcP), hexokinase (HK), glucose phosphate isomerase (GPI), and phosphoglucomutase (PGM), were used to compare the two isolates. All systems tested, apart from LDH, were found to be polymorphic for both isolates. Interestingly, one phenotype is more frequent than the remainder. The results show that there is no significant genetic variation between the S. mansoni isolates from man and the rodents, Arvicanthis niloticus and Mastomys huberti.

Is the determination of prostatic acid phosphatase still worthwhile in prostate cancer patients?

1997 ◽  
Vol 3 (2) ◽  
pp. 47-50
Author(s):  
Walter L Strohmaier ◽  
Andreas Zumbraegel ◽  
Lennart Koschella ◽  
K Horst Bichler
Keyword(s):  
Prostate Cancer ◽  
Acid Phosphatase ◽  
Cancer Patients ◽  
Prostatic Acid Phosphatase

Rapid Determination of Acid Phosphatase by a Surface Acoustic Wave Impedance Sensor

1996 ◽  
Vol 29 (1) ◽  
pp. 59-72 ◽  
Author(s):  
R. Wang ◽  
Q. Y. Cai ◽  
L. Wu ◽  
L. H. Nie ◽  
S. Z. Yao
Keyword(s):  
Acid Phosphatase ◽  
Acoustic Wave ◽  
Surface Acoustic Wave ◽  
Rapid Determination ◽  
Wave Impedance ◽  
Impedance Sensor

Determination of Acid Phosphatase Activity in Cells of Prostatic Tumours

Nature ◽  
1960 ◽  
Vol 188 (4751) ◽  
pp. 663-664 ◽  
Author(s):  
P. L. ESPOSTI ◽  
B. ESTBORN ◽  
J. ZAJICEK
Keyword(s):  
Acid Phosphatase ◽  
Phosphatase Activity ◽  
Acid Phosphatase Activity ◽  
In Cells

scholarly journals Determination of prostate cancer biomarker acid phosphatase at a copper phthalocyanine-modified screen printed gold transducer

2019 ◽  
Author(s):  
Zina Fredj ◽  
Mounir Ben Ali ◽  
Mohammed Nooredeen Abbas ◽  
Eithne Dempsey
Keyword(s):  
Prostate Cancer ◽  
Acid Phosphatase ◽  
Copper Phthalocyanine ◽  
Cancer Biomarker ◽  
Screen Printed

Simple, rapid determination of zinc and acid phosphatase in seminal plasma with an ABA-100 bichromatic analyzer.

1988 ◽  
Vol 34 (8) ◽  
pp. 1605-1607 ◽  
Author(s):  
M Gavella
Keyword(s):  
Acid Phosphatase ◽  
Male Infertility ◽  
Seminal Plasma ◽  
Rapid Determination ◽  
Assay System ◽  
Human Seminal Plasma ◽  
Automated Assay ◽  
Sensitivity Specificity

Abstract I describe an automated assay for zinc and acid phosphatase in seminal plasma. These, which are markers of the function of the prostate, were assayed bichromatically with an Abbott ABA-100 analyzer. As many as 25 samples of human seminal plasma can be analyzed sequentially with CVs of 3.1% for zinc and 1.5% for acid phosphatase. The sensitivity, specificity, and speed of this assay system make it practicable for use in investigation of male infertility.

scholarly journals Investigation by Isoelectric Focusing of the Initial Carbohydrate-deficient Transferrin (CDT) and non-CDT Transferrin Isoform Fractionation Step Involved in Determination of CDT by the ChronAlcoI.D. Assay

2000 ◽  
Vol 46 (4) ◽  
pp. 483-492 ◽  
Author(s):  
Rolf Hackler ◽  
Torsten Arndt ◽  
Angelika Helwig-Rolig ◽  
Juergen Kropf ◽  
Armin Steinmetz ◽  
...  
Keyword(s):  
Anion Exchange ◽  
Isoelectric Focusing ◽  
Peak Height ◽  
Test Results ◽  
Chronic Alcohol Abuse ◽  
Independent Evaluation ◽  
Carbohydrate Deficient Transferrin

Abstract Background: The introduction of a new set of reagents for the determination of carbohydrate-deficient transferrin (CDT) as a marker of chronic alcohol abuse requires an independent evaluation of the analytic specificity of the test. This information is needed for correct interpretation and classification of test results. Methods: Isoelectric focusing on the PhastSystemTM followed by immunofixation, silver staining, and densitometry was used to validate the initial transferrin isoform fractionation step on anion-exchange microcolumns involved in the ChronAlcoI.D.TM assay. Results: The in vitro transferrin iron load was complete and stable. The CDT and non-CDT transferrin fractionation on anion-exchange microcolumns was reliable and reproducible (CV ≤10%). Except for quantitatively unimportant traces of trisialo-Fe2-transferrin (<5% of total CDT), only asialo-, mono-, and disialo-Fe2-transferrin were detected in the microcolumn eluates (n = 170). There was a loss of proportionally similar amounts of asialo-Fe2-transferrin (during column rinsing) and disialo-Fe2-transferrin (on the anion exchanger). Thus, the peak height ratios for disialo- and asialo-Fe2-transferrin did not change from >1 (serum) to <1 (eluates) as described for the CDTect assays. The transferrin patterns in the ChronAlcoI.D. eluates were representative of those in serum. Transferrin D variants with isoelectric points close to that of trisialo-Fe2-transferrin C1 did not cause overdetermination of CDT by the ChronAlcoI.D. test. Conclusions: The initial CDT and non-CDT fractionation step involved in determination of CDT by the ChronAlcoI.D. assay is efficient for eliminating non-CDT transferrins from serum before quantification of CDT in the final turbidimetric immunoassay. We recommend IEF for validation of other (commercial) CDT analysis methods and of odd CDT results.

scholarly journals A Novel Immunoassay for The Determination of Tartrate-Resistant Acid Phosphatase 5b From Rat Serum

2003 ◽  
Vol 18 (1) ◽  
pp. 134-139 ◽  
Author(s):  
Sari L Alatalo ◽  
Zhiqi Peng ◽  
Anthony J Janckila ◽  
Helena Kaija ◽  
Pirkko Vihko ◽  
...  
Keyword(s):  
Acid Phosphatase ◽  
Tartrate Resistant Acid Phosphatase ◽  
Rat Serum
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