Potential of solid state fermentation for production of L(+)-lactic acid by Rhizopus oryzae

1994 ◽  
Vol 41 (3) ◽  
pp. 286-290 ◽  
Author(s):  
C. R. Soccol ◽  
B. Marin ◽  
M. Raimbault ◽  
J. -M. Lebeault
Keyword(s):  
Lactic Acid ◽  
Solid State ◽  
Solid State Fermentation ◽  
Rhizopus Oryzae

Potential of solid state fermentation for production of L(+)-lactic acid by Rhizopus oryzae

1994 ◽  
Vol 41 (3) ◽  
pp. 286-290 ◽  
Author(s):  
B. Marin ◽  
J.-M. Lebeault ◽  
M. Raimbault ◽  
C. R. Soccol
Keyword(s):  
Lactic Acid ◽  
Solid State ◽  
Solid State Fermentation ◽  
Rhizopus Oryzae

scholarly journals Evaluasi Perlakuan Pendahuluan Menggunakan Kalsium Hidroksida untuk Biokonversi Jerami Padi Menjadi L-Asam Laktat oleh Rhizopus oryzae AT3 (Evaluation of Lime Pretreatment for Bioconversion of Rice Straw to L-Lactic Acid by Rhizopus Oryzae AT3)

2016 ◽  
Vol 36 (03) ◽  
pp. 253
Author(s):  
Dhina Aprilia Nurani Widyahapsari ◽  
Retno Indrati ◽  
Sigit Setyabudi ◽  
Sardjono Sardjono
Keyword(s):  
Lactic Acid ◽  
Solid State ◽  
Enzymatic Hydrolysis ◽  
Rice Straw ◽  
Solid State Fermentation ◽  
Reducing Sugars ◽  
Rhizopus Oryzae ◽  
Crude Enzyme ◽  
Lime Pretreatment ◽  
Rice Straw Hydrolysate

L-lactic acid can be used as a precursor of polylactic acid (PLA). PLA is a biodegradable biomaterial commonly used for biodegradable plastics. Lactic acid can be produced from lignocelluloses materials such as rice straw. Rice straw is composed of cellulose and hemicellulose that can be hydrolyzed to fermentable sugar by cellulolytic and hemicellulolytic enzymes then converted to L-lactic acid by Rhizopus oryzae. As most cellulose and hemicellulose present in lignocellulose biomass are not readily accessible for these enzyme, pretreatment is required to alter the structure of lignocellulose substrates. This research aimed to investigate the effect of lime pretreatment on rice straw bioconversion to L-lactic acid by Rhizopus oryzae AT3. Rice straw was pretreated with lime (Ca(OH)2) at 85 °C for 16 hours. Unpretreated and pretreated rice straw were hydrolyzed using crude enzyme that produced by Trichoderma reesei Pk1J2. Enzyme production was carried out by solid state fermentation using rice straw and rice brand as substrate. Enzymatic hydrolysis was carried out in flasks. Each flask was added with unpretreated or pretreated rice straw, buffer citrate solution and crude enzyme then hydrolyzed for 0-96 hours. Hydrolysate was fermented by Rhizopus oryzae AT3 for 0-6 days by using adsorbed carrier solid-state fermentation method with polyurethane foam as inert support material. Lime pretreatment at 85 °C for 16 hour led to significant solubilisation of lignin and hemicellulose. It involved lignocellulose structure modified that enhance enzymatic hydrolysis and resulted higher reducing sugars than unpretreated rice straw. The high reducing sugars was not related to high lactic acid yields. Fermentation of pretreated rice straw hydrolysate by Rhizopus oryzae AT3 did not only produce L-lactic acid but also other compound. On the other hand, fermentation of unpretreated rice straw hydrolysate only produced L-lactic acid. ABSTRAKPolimerisasi asam laktat menjadi polylactic acid untuk menghasilkan biodegradable plastic membutuhkan asam laktat dengan isomer spesifik. Rhizopus oryzae adalah mikroorganisme yang spesifik menghasilkan L-asam laktat. Selain itu Rhizopus oryzae dapat menggunakan limbah pertanian seperti jerami padi sebagai substrat. Komponen utama jerami padi merupakan lignoselulosa yang dapat dihidrolisa secara enzimatis menjadi komponen gula sederhana penyusunnya dan selanjutnya dapat dikonversi menjadi L-asam laktat oleh Rhizopus oryzae. Namun struktur lignoselulosa sangat kompak dan rapat, sulit untuk dihidrolisa secara enzimatis sehingga diperlukan adanya perlakuan pendahuluan untuk merombak struktur lignoselulosa agar mudah dihidrolisa. Penelitian ini bertujuan untuk mengetahui pengaruh perlakuan pendahuluan menggunakan kalsium hidroksida (Ca(OH)2) terhadap biokonversi jerami padi menjadi L-asam laktat oleh Rhizopus oryzae AT3. Perlakuan pendahuluan pada jerami padi dilakukan menggunakan (Ca(OH)2) disertai pemanasan suhu 85 °C selama 16 jam. Jerami padi dengan dan tanpa perlakuan pendahuluan dihidrolisa secara enzimatis menggunakan crude enzyme yang diproduksi oleh Trichoderma reesei Pk1J2. Produksi crude enzyme dilakukan dengan fermentasi substrat padat dengan campuran jerami padi dan dedak sebagai substrat. Hidrolisat jerami padi dengan dan tanpa perlakuan pendahuluan selanjutnya difermentasi oleh Rhizopus oryzae AT3 menggunakan metode adsorbed carrier solid state fermentation dengan polyurethane foam (PUF) sebagai bahan pendukung. Perlakuan pendahuluan menggunakan Ca(OH2) disertai pemanasan suhu 85 °C selama 16 jam dapat merubah komposisi lignoselulosa jerami padi yaitu dengan melarutkan lignin dan hemiselulosa. Perubahan komposisi lignoselulosa memudahkan kerja crude enzyme dalam menghidrolisa jerami padi sehingga menghasilkan gula reduksi lebih tinggi dibandingkan jerami padi tanpa perlakuan pendahuluan. Tingginya gula reduksi tidak serta merta meningkatkan yield L-asam laktat yang dihasilkan. Fermentasi hidrolisat jerami padi dengan perlakuan pendahuluan oleh Rhizopus oryzae AT3 menghasilkan yield L-asam laktat lebih rendah dibandingkan hidrolisat jerami padi tanpa perlakuan pendahuluan. Namun pada jerami padi dengan perlakuan pendahuluan dihasilkan senyawa lain selain asam laktat.Kata Kunci: Adsorbed carrier solid state fermentation; L-asam laktat; perlakuan pendahuluan; Rhizopus oryzae AT3; jerami padi

DETERMINATION OF LACTIC ACID PRODUCTION BY RHIZOPUS ORYZAE IN SOLID STATE FERMENTATION OF PINEAPPLE WASTE

2015 ◽  
Vol 77 (31) ◽  
Author(s):  
Siti Nurbalqis Aziman ◽  
Hasnaa’ Hazimah Tumari ◽  
Nor Azimah Mohd Zain
Keyword(s):  
Lactic Acid ◽  
Solid State ◽  
Solid State Fermentation ◽  
Acid Production ◽  
Rhizopus Oryzae ◽  
Lactic Acid Production ◽  
Inoculum Size ◽  
Pineapple Waste ◽  
Good Agreement

Solid pineapple waste (SPW) is one of the most abundant agricultural wastes found in tropic region. This study is looking into the potential of utilising solid pineapple waste in solid state fermentation for the production of lactic acid by Rhizopus oryzae. A 2-level factorial design was employed to screen the effect of moisture content (60% and 80%), inoculum size (1×104 spores/g and 1×108 spores/g), pH (4.5 and 6.5), temperature (27°C and 40°C) and particle size (<0.5 mm and >3.15 mm) to the production of lactic acid. The predicted maximum production is 0.0221 g lactic acid/g SPW in SSF condition of 80% moisture; pH 6.5; 1×104 spores/g of inoculum; waste particle of 3.15 mm; and temperature 27°C. Analysis of variance (ANOVA) showed that the model is significant with high value of predicted (0.9616) and adjusted (0.9726) R-squared, indicated a good agreement between the predicted and actual values at each point of the experiment. Post-statistical experiment confirmed the ability of lactic acid production by R. oryzae at the predicted conditions with 0.0236 g lactic acid/g SPW being produced.

L(+)-Lactic Acid Production Using Lactobacillus Casei in Solid-State Fermentation

2005 ◽  
Vol 27 (21) ◽  
pp. 1685-1688 ◽  
Author(s):  
Pappy John Rojan ◽  
Kesavan Madhavan Nampoothiri ◽  
Athira Syamaprasad Nair ◽  
Ashok Pandey
Keyword(s):  
Lactic Acid ◽  
Solid State ◽  
Solid State Fermentation ◽  
Acid Production ◽  
Lactobacillus Casei ◽  
Lactic Acid Production

scholarly journals Carboxylic acid production from ensiled crops in anaerobic solid-state fermentation - trace elements as pH controlling agents support microbial chain elongation with lactic acid

2018 ◽  
Vol 18 (7) ◽  
pp. 447-458 ◽  
Author(s):  
Heike Sträuber ◽  
Franziska Bühligen ◽  
Sabine Kleinsteuber ◽  
Michael Dittrich-Zechendorf
Keyword(s):  
Trace Elements ◽  
Lactic Acid ◽  
Solid State ◽  
Carboxylic Acid ◽  
Solid State Fermentation ◽  
Acid Production ◽  
Chain Elongation

Optimization of soy solid-state fermentation with selected lactic acid bacteria and the effect on the anti-nutritional components

2017 ◽  
Vol 41 (6) ◽  
pp. e13290 ◽  
Author(s):  
Xin Rui ◽  
Mingjia Wang ◽  
Yuqian Zhang ◽  
Xiang Chen ◽  
Lan Li ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Solid State ◽  
Lactic Acid Bacteria ◽  
Solid State Fermentation ◽  
Nutritional Components

scholarly journals A Statistical Approach for Optimization of Simultaneous Production of β-Glucosidase and Endoglucanase by Rhizopus oryzae from Solid-State Fermentation of Water Hyacinth Using Central Composite Design

2011 ◽  
Vol 2011 ◽  
pp. 1-7 ◽  
Author(s):  
Moumita Karmakar ◽  
Rina Rani Ray
Keyword(s):  
Solid State ◽  
Carbon Source ◽  
Solid State Fermentation ◽  
Substrate Concentration ◽  
Water Hyacinth ◽  
Enzyme Production ◽  
Rhizopus Oryzae ◽  
Aquatic Weed ◽  
The Impact ◽  
Central Composite

The production cost of β-glucosidase and endoglucanase could be reduced by using water hyacinth, an aquatic weed, as the sole carbon source and using cost-efficient fermentation strategies like solid-state fermentation (SSF). In the present study, the effect of different production conditions on the yield of β-glucosidase and endoglucanase by Rhizopus oryzae MTCC 9642 from water hyacinth was investigated systematically using response surface methodology. A Central composite experimental design was applied to optimize the impact of three variables, namely, substrate concentration, pH, and temperature, on enzyme production. The optimal level of each parameter for maximum enzyme production by the fungus was determined. Highest activity of endoglucanase of 495 U/mL was achieved at a substrate concentration of 1.23%, pH 7.29, and temperature 29.93°C whereas maximum β-glucosidase activity of 137.32 U/ml was achieved at a substrate concentration of 1.25%, pH 6.66, and temperature 32.09°C. There was a direct correlation between the levels of enzymatic activities and the substrate concentration of water hyacinth as carbon source.

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