scholarly journals A Statistical Approach for Optimization of Simultaneous Production of β-Glucosidase and Endoglucanase by Rhizopus oryzae from Solid-State Fermentation of Water Hyacinth Using Central Composite Design

2011 ◽  
Vol 2011 ◽  
pp. 1-7 ◽  
Author(s):  
Moumita Karmakar ◽  
Rina Rani Ray
Keyword(s):  
Solid State ◽  
Carbon Source ◽  
Solid State Fermentation ◽  
Substrate Concentration ◽  
Water Hyacinth ◽  
Enzyme Production ◽  
Rhizopus Oryzae ◽  
Aquatic Weed ◽  
The Impact ◽  
Central Composite

The production cost of β-glucosidase and endoglucanase could be reduced by using water hyacinth, an aquatic weed, as the sole carbon source and using cost-efficient fermentation strategies like solid-state fermentation (SSF). In the present study, the effect of different production conditions on the yield of β-glucosidase and endoglucanase by Rhizopus oryzae MTCC 9642 from water hyacinth was investigated systematically using response surface methodology. A Central composite experimental design was applied to optimize the impact of three variables, namely, substrate concentration, pH, and temperature, on enzyme production. The optimal level of each parameter for maximum enzyme production by the fungus was determined. Highest activity of endoglucanase of 495 U/mL was achieved at a substrate concentration of 1.23%, pH 7.29, and temperature 29.93°C whereas maximum β-glucosidase activity of 137.32 U/ml was achieved at a substrate concentration of 1.25%, pH 6.66, and temperature 32.09°C. There was a direct correlation between the levels of enzymatic activities and the substrate concentration of water hyacinth as carbon source.

scholarly journals Enzyme production by soil filamentous fungi under solid-state fermentation in banana stalk

2018 ◽  
Vol 8 (3) ◽  
pp. 424-431 ◽  
Author(s):  
Fernanda Castro Pires dos Santos ◽  
Joice Raísa Barbosa Cunha ◽  
Fábia Giovana Do val de Assis ◽  
Patrícia Lopes Leal
Keyword(s):  
Solid State ◽  
Environmental Factors ◽  
Solid State Fermentation ◽  
Substrate Concentration ◽  
Enzyme Production ◽  
Agricultural Waste ◽  
Cellulolytic Enzymes ◽  
Sustainable Solutions ◽  
Research Challenge ◽  
Penicillium Spp

The adequate disposal of agricultural waste is one of the major concerns of public officials and a research challenge to obtain sustainable solutions to the problem. In this sense, the objective of this study was to evaluate the banana leaf stalk use as substrate in solid-state fermentation (SSF) for production of amylolytic and cellulolytic enzymes by Penicillium spp. LEMI A11 strain grown under different substrate concentrations, pH and temperature. Effects of different pH conditions (5.0 and 6.0), temperature (30 and 35 °C) and substrate concentration 70 and 90% (in relation to the final volume) of the fermentation were evaluated over 120 hours of fermentation. The results indicated that Penicillium spp. LEMI A11 was able to use the banana stalk as substrate under SSF. The maximum activities for amylase dextraining, amylase saccharifying and CMCase were 0.18; 0.13 and 04 U.g-1, respectively. The effect of environmental factors related to the substrate concentration was significant for saccharifying amylase and CMCase activity only. The interaction between the environmental factors tested was significant for the dextrinizing amylase activity only. It was verified enzyme activity reduction after 96 hours of fermentation for all enzymes. It concluded that banana stalk is an alternative carbon source to be used in SSF for enzyme production by of Penicillium spp. LEMI A11.

scholarly journals Biotechnological Potential of Agro Residues for Economical Production of Thermoalkali-Stable Pectinase by Bacillus pumilus dcsr1 by Solid-State Fermentation and Its Efficacy in the Treatment of Ramie Fibres

2012 ◽  
Vol 2012 ◽  
pp. 1-7 ◽  
Author(s):  
Deepak Chand Sharma ◽  
T. Satyanarayana
Keyword(s):  
Solid State ◽  
Solid State Fermentation ◽  
Enzyme Production ◽  
Bacillus Pumilus ◽  
Biotechnological Potential ◽  
Oilseed Cake ◽  
The Impact ◽  
Modulus Reduction ◽  
Agro Residues

The production of a thermostable and highly alkaline pectinase by Bacillus pumilus dcsr1 was optimized in solid-state fermentation (SSF) and the impact of various treatments (chemical, enzymatic, and in combination) on the quality of ramie fibres was investigated. Maximum enzyme titer ( Ug−1 DBB) in SSF was attained, when a mixture of agro-residues (sesame oilseed cake, wheat bran, and citrus pectin, 1 : 1 : 0.01) was moistened with mineral salt solution ( 0.92, pH 9.0) at a substrate-to-moistening agent ratio of 1 : 2.5 and inoculated with 25% of 24 h old inoculum, in 144 h at 40°C. Parametric optimization in SSF resulted in 1.7-fold enhancement in the enzyme production as compared to that recorded in unoptimized conditions. A 14.2-fold higher enzyme production was attained in SSF as compared to that in submerged fermentation (SmF). The treatment with the enzyme significantly improved tensile strength and Young’s modulus, reduction in brittleness, redness and yellowness, and increase in the strength and brightness of ramie fibres.

scholarly journals Comparative Evaluation of Agroindustrial Byproducts for the Production of Alkaline Protease by Wild and Mutant Strains ofBacillus subtilisin Submerged and Solid State Fermentation

2013 ◽  
Vol 2013 ◽  
pp. 1-6 ◽  
Author(s):  
Hamid Mukhtar ◽  
Ikramul Haq
Keyword(s):  
Bacillus Subtilis ◽  
Solid State ◽  
Wheat Bran ◽  
Solid State Fermentation ◽  
Soybean Meal ◽  
Alkaline Protease ◽  
Enzyme Production ◽  
Enhanced Production ◽  
Industrial Byproducts ◽  
Agroindustrial Byproducts

The present study describes the screening of different agroindustrial byproducts for enhanced production of alkaline protease by a wild and EMS induced mutant strain ofBacillus subtilisIH-72EMS8. During submerged fermentation, different agro-industrial byproducts were tested which include defatted seed meals of rape, guar, sunflower, gluten, cotton, soybean, and gram. In addition to these meals, rice bran, wheat bran, and wheat flour were also evaluated for protease production. Of all the byproducts tested, soybean meal at a concentration of 20 g/L gave maximum production of the enzyme, that is, 5.74  ±  0.26 U/mL from wild and 11.28  ±  0.45 U/mL from mutant strain, during submerged fermentation. Different mesh sizes (coarse, medium, and fine) of the soybean meal were also evaluated, and a finely ground soybean meal (fine mesh) was found to be the best. In addition to the defatted seed meals, their alkali extracts were also tested for the production of alkaline protease byBacillus subtilis, but these were proved nonsignificant for enhanced production of the enzyme. The production of the enzyme was also studied in solid state fermentation, and different agro-industrial byproducts were also evaluated for enzyme production. Wheat bran partially replaced with guar meal was found as the best substrate for maximum enzyme production under solid state fermentation conditions.

scholarly journals Screening of winery and olive mill wastes for lignocellulolytic enzyme production from Aspergillus species by solid-state fermentation

2013 ◽  
Vol 4 (3) ◽  
pp. 201-209 ◽  
Author(s):  
José Manuel Salgado ◽  
Luís Abrunhosa ◽  
Armando Venâncio ◽  
José Manuel Domínguez ◽  
Isabel Belo
Keyword(s):  
Solid State ◽  
Solid State Fermentation ◽  
Enzyme Production ◽  
Aspergillus Species ◽  
Olive Mill Wastes ◽  
Olive Mill

scholarly journals APROVEITAMENTO DE RESÍDUOS AGROINDUSTRIAIS: PRODUÇÃO DE ENZIMAS A PARTIR DA CASCA DE COCO VERDE

2001 ◽  
Vol 19 (1) ◽  
Author(s):  
MARIA ALICE ZARUR COELHO ◽  
SELMA GOMES FERREIRA LEITE ◽  
MORSYLEIDE DE FREITAS ROSA ◽  
ANGELA APARECIDA LEMOS FURTADO
Keyword(s):  
Solid State ◽  
Aspergillus Niger ◽  
Solid State Fermentation ◽  
Industrial Production ◽  
Enzyme Production ◽  
Raw Material ◽  
Production Time ◽  
Maximum Production ◽  
E 32

Investigou-se o aproveitamento da casca do coco verde, mediante fermentação semisólida, para produção de enzimas. A casca de coco foi previamente desidratada, moída e classificada em três diferentes granulometrias, ou seja, 14, 28 e 32 mesh Tyler. Todas as enzimas obtidas tiveram sua produção máxima na faixa de 24 e 96 horas, o que corresponde ao tempo de produção industrial corrente. Cada granulometria produziu complexos enzimáticos ricos em diferentes atividades. O estudo realizado validou a hipótese do aproveitamento do resíduo da casca do coco verde na produção de enzimas por Aspergillus niger. Abstract The utilization of immature coconut peel as substrate for enzyme production by solid state fermentation was investigated. The coconut peel was previously dehydrated, milled and classified in three distinct granulometries: 14, 28 and 32 mesh Tyler. All the enzymes obtained had its maximum production in 24 to 96 hour interval, which correspond to the current industrial production time. Each granulometry produced rich enzymatic complexes with different activities. This study validates the hypothesis of benefit immature coconut peel as raw material for enzyme production by Aspergillus niger.

Sign in / Sign up

Export Citation Format

Share Document