Factors affecting transient gene expression in protoplasts isolated from very slowly growing embryogenic callus cultures of wheat (Triticum aestivum L.)

1993 ◽  
Vol 86 (6) ◽  
pp. 721-730 ◽  
Author(s):  
O. M. -F. Zaghmout ◽  
N. L. Trolinder
Keyword(s):  
Gene Expression ◽  
Triticum Aestivum ◽  
Embryogenic Callus ◽  
Transient Gene Expression ◽  
Triticum Aestivum L ◽  
Callus Cultures ◽  
Factors Affecting

scholarly journals Receiving of genetic-modified wheat plants with heterolous ornitin-δ-aminotransferase gene

2018 ◽  
Vol 22 ◽  
pp. 222-227
Author(s):  
O. M. Honcharuk ◽  
O. V. Dubrovna
Keyword(s):  
Triticum Aestivum ◽  
Transgenic Plants ◽  
Bread Wheat ◽  
Binary Vector ◽  
Triticum Aestivum L ◽  
Callus Cultures ◽  
Pcr Analysis ◽  
Genetic Construct ◽  
Agrobacterium Mediated Transformation

Aim. Receiving of genetically modified plants of bread wheat with heterologous ornithine‑δ‑aminotransferase gene. Methods. Agrobacterium-mediated transformation of callus cultures in vitro, PCR-analysis. Results. By Agrobacterium-mediated transformation of the morphogenic calluses of bread wheat (Triticum aestivum L.) using the AGLO strain containing the binary vector pBi-OAT with the target ornithine-δ-aminotransferase (oat) and selective neomycinphosphotransferase II (nptII), transgenic plants-regenerators have been obtained. Conclusions. As a result of the genetic transformation of Zimoyarka variety, 12 wheat regenerants were obtained in the genome which revealed a complete integration of the genetic construct containing the oat and nptII transgenes. Keywords: Triticum aestivum L., Agrobacterium-mediated transformation, ornithine‑δ‑aminotransferase gene, PCR-analysis.

Factors affecting transient gene expression in cultured radiata pine cotyledons following particle bombardment

1996 ◽  
Vol 96 (4) ◽  
pp. 630-636 ◽  
Author(s):  
Manuel Rey ◽  
Maria Victoria Gonzalez ◽  
Ricardo J. Ordas ◽  
Raffaela Tavazza ◽  
Giorgio Ancora
Keyword(s):  
Gene Expression ◽  
Particle Bombardment ◽  
Transient Gene Expression ◽  
Radiata Pine ◽  
Factors Affecting

Em gene expression during somatic embryogenesis in the monocot Triticum aestivum L.

Plant Science ◽  
1996 ◽  
Vol 117 (1-2) ◽  
pp. 139-149 ◽  
Author(s):  
Fabienne Corre ◽  
Yves Henry ◽  
André Rode ◽  
Caroline Hartmann
Keyword(s):  
Gene Expression ◽  
Triticum Aestivum ◽  
Somatic Embryogenesis ◽  
Triticum Aestivum L ◽  
Em Gene

scholarly journals Callus Induction and Regeneration from Germinating Mature Embryos of Wheat (Triticum aestivum L.)

2021 ◽  
Vol 50 (4) ◽  
pp. 889-896
Author(s):  
Samih M. Tamimi ◽  
Halima Othman
Keyword(s):  
Triticum Aestivum ◽  
Callus Induction ◽  
High Performance ◽  
Triticum Aestivum L ◽  
Mature Embryo ◽  
Callus Cultures ◽  
Differentiation Potential ◽  
Mature Embryos ◽  
The Mean ◽  
Wheat Callus

A high-performance protocol for callus induction was devised using germinating mature embryos of two local wheat (Triticum aestivum L.) landraces as explant. The results showed that callus development from germinating embryos was rapid starting one day after culture with an induction rate 20 to 25% higher than those of soaked embryos. In addition, the mean rate of growth of callus developed from germinating embryos was 60 to 70% higher than those cultured from soaked embryos. This study also demonstrated a higher frequency of green spots formation(48 to 56%)on callus derived from germinating embryos compared to their soaked counterpart (24 to 28%), suggesting a better differentiation potential of callus cultures derived from germinating embryo. These findings indicate that germinating mature embryo is more suitable explant for wheat callus induction and regeneration than the soaked mature embryo commonly employed for wheat callus culture.

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