Use of RAPD markers to determine the genetic diversity of diploid, wheat genotypes

1992 ◽  
Vol 84-84 (7-8) ◽  
pp. 835-838 ◽  
Author(s):  
Richard A. Vierling ◽  
Henry T. Nguyen
Keyword(s):  
Genetic Diversity ◽  
Rapd Markers ◽  
Diploid Wheat ◽  
Wheat Genotypes

scholarly journals Assessment of genetic diversity in bread wheat (Triticum aestivum L.) using RAPD markers

2017 ◽  
Vol 9 (3) ◽  
pp. 1751-1755
Author(s):  
Amit Kumar ◽  
R. S. Sengar ◽  
Vivekanand Pratap Rao ◽  
Gyanika Shukla ◽  
Rekha Dixit ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Cluster Analysis ◽  
Genomic Dna ◽  
Core Collection ◽  
Rapd Markers ◽  
Rapd Marker ◽  
Triticum Aestivum L ◽  
Wheat Cultivars ◽  
High Genetic Diversity ◽  
Wheat Genotypes

The present study aimed to evaluate the genetic diversity of 10 wheat cultivars by Random Amplified Pol-ymorphic DNA (RAPD) marker. The genomic DNA of 10 wheat genotypes were amplified with 10 RAPD primers that produced 53 amplified band, out of which 23 band were polymorphic (43.39%). The number of fragment amplified per primer ranged from 4 to 9. Primer A01 generated maximum number of amplified band, out of which 5 band were polymorphic. Cluster analysis of wheat genotypes were based on UPGMA method. Cluster analysis of 10 wheat genotypes were classified in to two main group; single variety AKW 1071 was placed in first group and rest 9 variety were placed in second group. The pair wise similarity values ranged from 0.58% to 100% and showed that cultivars Raj-3765 and K-7903 were the closest with highest similarity value (100%), while genotypes AKW 1071 and K9006 showed minimum similarity value (62%). The present study indicated the presence of high genetic diversity among wheat cultivars, which could be used for the developing core collection of wheat germplasm for breeding purpose.

Genetic diversity of durum wheat genotypes using morpho-protein analysis

2014 ◽  
Vol 42 (4) ◽  
pp. 677-686
Author(s):  
M. Rajabi Hashjin ◽  
M.H. Fotokian ◽  
M. Agahee Sarbrzeh ◽  
M. Mohammadi ◽  
D. Talei
Keyword(s):  
Genetic Diversity ◽  
Durum Wheat ◽  
Protein Analysis ◽  
Wheat Genotypes

Assessing temporal changes in genetic diversity of maize hybrids using RAPD markers

2007 ◽  
Vol 35 (4) ◽  
pp. 1563-1571 ◽  
Author(s):  
I. Bauer ◽  
S. Mladenovic Drinic ◽  
G. Drinić ◽  
D. Ignjatović Micić
Keyword(s):  
Genetic Diversity ◽  
Rapd Markers ◽  
Temporal Changes ◽  
Maize Hybrids

Study of genetic diversity in wild raspberry (Rubus idaeus L.) germplasm collection using morphological characters and RAPD markers

Biologija ◽  
2008 ◽  
Vol 54 (2) ◽  
pp. 66-74 ◽  
Author(s):  
Jolanta Patamsytė ◽  
Donatas Žvingila ◽  
Juozas Labokas ◽  
Virgilijus Baliuckas ◽  
Laimutė Balčiūnienė ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Rapd Markers ◽  
Germplasm Collection ◽  
Morphological Characters ◽  
Rubus Idaeus

Genetic Diversity of Basil (Ocimum spp. - Lamiaceae) Based on RAPD Markers and Volatile Oil Constituents

HortScience ◽  
1998 ◽  
Vol 33 (3) ◽  
pp. 492f-493
Author(s):  
Roberto F. Vieira ◽  
James E. Simon ◽  
Peter Goldsbrough ◽  
Antonio Figueira
Keyword(s):  
Genetic Diversity ◽  
Molecular Markers ◽  
Rapd Markers ◽  
Volatile Oil ◽  
Essential Oil Composition ◽  
Oil Composition ◽  
Traditional Medicines ◽  
The Tropics ◽  
High Degree ◽  
First Time

Essential oils extracted from basil (Ocimum spp.) by steam distillation are used to flavor foods, oral products, in fragrances, and in traditional medicines. The genus Ocimum contains around 30 species native to the tropics and subtropics, with some species naturalized and/or cultivated in temperate areas. Interand intraspecific hybridization have created significant confusion in the botanical systematics of this genus. Taxonomy of basil (O. basilicum) is also complicated by the existence of numerous varieties, cultivars, and chemotypes within the species that do not differ significantly in morphology. In this study we are using RAPD markers and volatile oil composition to characterize the genetic diversity among the most economically important Ocimum species. We hypothesize that the genetic similarity revealed by molecular markers will more accurately reflect the morphological and chemical differences in Ocimum than essential oil composition per se. Preliminary research using five Ocimum species, four undetermined species, and eight varieties of O. basilicum (a total of 19 accessions) generated 107 polymorphic fragments amplified with 19 primers. RAPDs are able to discriminate between Ocimum species, but show a high degree of similarity between O. basilicum varieties. The genetic distance between nine species and among 55 accessions within the species O. americanum, O. basilicum, O. campechianum, O. × citriodorum, O. gratissimum, O. kilimandscharium, O. minimum, O. selloi, and O. tenuiflorum will be analyzed by matrix of similarity and compared to the volatile oil profile. This research will for the first time apply molecular markers to characterize the genetic diversity of Ocimum associate with volatile oil constituent.

The Impact of Drought Stress on some Morpho-Physiological Traits and RAPD Markers in Wheat Genotypes

2015 ◽  
Vol 4 (1) ◽  
pp. 27-37 ◽  
Author(s):  
Bayoumi Y. ◽  
Amal Abd EL-Mageed ◽  
Enas Ibrahim ◽  
Soad Mahmoud ◽  
I. El-Demardash ◽  
...  
Keyword(s):  
Drought Stress ◽  
Rapd Markers ◽  
Physiological Traits ◽  
Wheat Genotypes ◽  
The Impact

Assessment of the Genetic Diversity in Iranian Fennels by RAPD Markers

2013 ◽  
Vol 19 (3) ◽  
pp. 275-285 ◽  
Author(s):  
Kaivan Bahmani ◽  
Ali Izadi Darbandi ◽  
Seyed Ahmad Sadat Noori ◽  
Ali Ashraf Jafari
Keyword(s):  
Genetic Diversity ◽  
Rapd Markers

Genetic diversity of Meloidogyne spp. parasitising potato in Brazil and aggressiveness of M. javanica populations on susceptible cultivars

Nematology ◽  
2017 ◽  
Vol 19 (1) ◽  
pp. 69-80 ◽  
Author(s):  
Israel L. Medina ◽  
Cesar B. Gomes ◽  
Valdir R. Correa ◽  
Vanessa S. Mattos ◽  
Philippe Castagnone-Sereno ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Rapd Markers ◽  
Potato Production ◽  
Potato Cultivars ◽  
Root Knot Nematode ◽  
Root Knot Nematodes ◽  
Nematode Reproduction ◽  
Meloidogyne Spp ◽  
Prevalent Species ◽  
Reproduction Factor

Root-knot nematodes (Meloidogyne spp.) significantly impact potato production worldwide and in Brazil they are considered one of the most important group of nematodes affecting potatoes. The objectives of this study were to survey Meloidogyne spp. associated with potatoes in Brazil, determine their genetic diversity and assess the aggressiveness of M. javanica on two susceptible potato cultivars. Fifty-seven root-knot nematode populations were identified using esterase phenotyping, including Meloidogyne javanica, M. incognita, M. arenaria and M. ethiopica. Overall, root-knot nematodes were present in ca 43% of sampled sites, in which M. javanica was the most prevalent species, and the phenotypes Est J3, J2a and J2 occurred in 91.2, 6.7 and 2.1% of the positive samples, respectively. Other species, such as M. incognita, M. arenaria and M. ethiopica, were found less frequently and occurred at rates of 6.4, 4.3 and 2.1% of the samples, respectively. Sometimes, M. javanica was found in mixtures with other root-knot nematodes in ca 10.6% of sites containing Meloidogyne. After confirming the identification of 17 isolates of M. javanica and one isolate each of M. incognita, M. arenaria and M. ethiopica by SCAR markers, the populations were used to infer their genetic diversity using RAPD markers. Results revealed low intraspecifc genetic diversity among isolates (13.9%) for M. javanica. Similarly, M. javanica sub-populations (J2a) clustered together (81% of bootstrap), indicating subtle variation from typical J3 populations. The aggressiveness of four populations of M. javanica from different Brazilian states on two susceptible potato cultivars was tested under glasshouse conditions. Results indicated differences in aggressiveness among these populations and showed that potato disease was proportional to nematode reproduction factor.

Genetic diversity in Bambara groundnut (Vigna subterranea L.) germplasm revealed by RAPD markers

Genome ◽  
2001 ◽  
Vol 44 (6) ◽  
pp. 995-999 ◽  
Author(s):  
H I Amadou ◽  
P J Bebeli ◽  
P J Kaltsikes
Keyword(s):  
Genetic Diversity ◽  
Cluster Analysis ◽  
Rapd Markers ◽  
Random Amplified Polymorphic Dna ◽  
Geographic Origin ◽  
Bambara Groundnut ◽  
International Institute ◽  
Tropical Agriculture ◽  
Vigna Subterranea ◽  
Ibadan Nigeria

Random amplified polymorphic DNA (RAPD) markers were used to assess genetic diversity in Bambara groundnut (Vigna subterranea L.) germplasm using 25 African accessions from the collection in the International Institute for Tropical Agriculture, Ibadan, Nigeria. Fifty random decamer primers were screened to assess their ability to detect polymorphism in bambara; 17 of them were selected for this study. Considerable genetic diversity was found among the V. subterranea accessions studied. The relationships among the 25 accessions were studied by cluster analysis. The dendrograms showed two main groups of accessions mainly along the lines of their geographic origin. It is concluded that RAPD can be used for germplasm classification in bambara groundnut and hence for improving this crop.Key words: germplasm, PCR, RAPD, Vigna subterranea.

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