tra cistrons and proteins encoded by the Escherichia coli antibiotic resistance plasmid R6-5

1978 ◽  
Vol 163 (2) ◽  
pp. 169-179 ◽  
Author(s):  
Mark Achtman ◽  
Barica Kušećek ◽  
Kenneth N. Timmis
Keyword(s):  
Escherichia Coli ◽  
Antibiotic Resistance ◽  
Resistance Plasmid

scholarly journals Limited and strain-specific transcriptional and growth responses to acquisition of a multidrug resistance plasmid in genetically diverse Escherichia coli lineages

2020 ◽  
Author(s):  
Steven J. Dunn ◽  
Laura Carrilero ◽  
Michael Brockhurst ◽  
Alan McNally
Keyword(s):  
Escherichia Coli ◽  
Antibiotic Resistance ◽  
Multidrug Resistance ◽  
Resistance Genes ◽  
Antibiotic Resistance Genes ◽  
Growth Responses ◽  
Fitness Costs ◽  
Transcriptional Responses ◽  
E Coli ◽  
Resistance Plasmid

AbstractMulti-drug resistant (MDR) Escherichia coli are a major global threat to human health, wherein multi-drug resistance is primarily spread by MDR plasmid acquisition. MDR plasmids are not widely distributed across the entire E. coli species, but instead are concentrated in a small number of clones. Here, we test if diverse E. coli strains vary in their ability to acquire and maintain MDR plasmids, and if this relates to their transcriptional response following plasmid acquisition. We used strains from across the diversity of E. coli, including the common MDR lineage ST131, and the IncF plasmid, pLL35, encoding multiple antibiotic resistance genes. Strains varied in their ability to acquire pLL35 by conjugation, but all were able to stably maintain the plasmid. The effects of pLL35 acquisition on cefotaxime resistance and growth also varied among strains, with growth responses ranging from a small decrease to a small increase in growth of the plasmid-carrier relative to the parental strain. Transcriptional responses to pLL35 acquisition were limited in scale and highly strain specific. We observed significant transcriptional responses at the operon or regulon level, possibly due to stress responses or interactions with resident MGEs. Subtle transcriptional responses consistent across all strains were observed affecting functions, such as anaerobic metabolism, previously shown to be under negative frequency dependent selection in MDR E. coli. Overall there was no correlation between the magnitude of the transcriptional and growth responses across strains. Together these data suggest that fitness costs arising from transcriptional disruption are unlikely to act as a barrier to MDR plasmid dissemination in E. coli.ImportancePlasmids play a key role in bacterial evolution by transferring niche adaptive functions between lineages, including driving the spread of antibiotic resistance genes. Fitness costs of plasmid acquisition arising from the disruption of cellular processes could limit the spread of multidrug resistance plasmids. However, the impacts of plasmid acquisition are typically measured in lab-adapted strains rather than in more ecologically relevant natural isolates. Using a clinical multidrug resistance plasmid and a diverse collection of E. coli strains isolated from clinical infections and natural environments, we show that plasmid acquisition had only limited and highly strain-specific effects on bacterial growth and transcription. These findings suggest that fitness costs arising from transcriptional disruption are unlikely to act as a barrier to plasmid transmission in natural populations of E. coli.

scholarly journals Stability in Escherichia coli of an antibiotic resistance plasmid from Bacteroides fragilis.

1981 ◽  
Vol 146 (1) ◽  
pp. 121-127 ◽  
Author(s):  
A Rashtchian ◽  
S J Booth
Keyword(s):  
Escherichia Coli ◽  
Antibiotic Resistance ◽  
Bacteroides Fragilis ◽  
Resistance Plasmid

scholarly journals Molecular Characterization of a 21.4 Kilobase Antibiotic Resistance Plasmid from an α-Hemolytic Escherichia coli O108:H- Human Clinical Isolate

PLoS ONE ◽  
2012 ◽  
Vol 7 (4) ◽  
pp. e34718
Author(s):  
Fay E. Dawes ◽  
Dieter M. Bulach ◽  
Alexander Kuzevski ◽  
Karl A. Bettelheim ◽  
Carola Venturini ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Antibiotic Resistance ◽  
Molecular Characterization ◽  
Clinical Isolate ◽  
Resistance Plasmid

scholarly journals Characterization of a Large Antibiotic Resistance Plasmid Found in Enteropathogenic Escherichia coli Strain B171 and Its Relatedness to Plasmids of Diverse E. coli and Shigella Strains

2017 ◽  
Vol 61 (9) ◽  
Author(s):  
Tracy H. Hazen ◽  
Jane Michalski ◽  
Sushma Nagaraj ◽  
Iruka N. Okeke ◽  
David A. Rasko
Keyword(s):  
Escherichia Coli ◽  
Antibiotic Resistance ◽  
Virulence Plasmid ◽  
Type I ◽  
Epec Strain ◽  
Content Type ◽  
E Coli ◽  
Resistance Plasmid ◽  
Resistance Plasmids

ABSTRACT Enteropathogenic Escherichia coli (EPEC) is a leading cause of severe infantile diarrhea in developing countries. Previous research has focused on the diversity of the EPEC virulence plasmid, whereas less is known regarding the genetic content and distribution of antibiotic resistance plasmids carried by EPEC. A previous study demonstrated that in addition to the virulence plasmid, reference EPEC strain B171 harbors a second, larger plasmid that confers antibiotic resistance. To further understand the genetic diversity and dissemination of antibiotic resistance plasmids among EPEC strains, we describe the complete sequence of an antibiotic resistance plasmid from EPEC strain B171. The resistance plasmid, pB171_90, has a completed sequence length of 90,229 bp, a GC content of 54.55%, and carries protein-encoding genes involved in conjugative transfer, resistance to tetracycline (tetA), sulfonamides (sulI), and mercury, as well as several virulence-associated genes, including the transcriptional regulator hha and the putative calcium sequestration inhibitor (csi). In silico detection of the pB171_90 genes among 4,798 publicly available E. coli genome assemblies indicates that the unique genes of pB171_90 (csi and traI) are primarily restricted to genomes identified as EPEC or enterotoxigenic E. coli. However, conserved regions of the pB171_90 plasmid containing genes involved in replication, stability, and antibiotic resistance were identified among diverse E. coli pathotypes. Interestingly, pB171_90 also exhibited significant similarity with a sequenced plasmid from Shigella dysenteriae type I. Our findings demonstrate the mosaic nature of EPEC antibiotic resistance plasmids and highlight the need for additional sequence-based characterization of antibiotic resistance plasmids harbored by pathogenic E. coli.

scholarly journals Persistence of an antibiotic resistance plasmid in intestinal Escherichia coli of chickens in the absence of selective pressure.

1987 ◽  
Vol 31 (5) ◽  
pp. 784-788 ◽  
Author(s):  
E Chaslus-Dancla ◽  
G Gerbaud ◽  
M Lagorce ◽  
J P Lafont ◽  
P Courvalin
Keyword(s):  
Escherichia Coli ◽  
Antibiotic Resistance ◽  
Selective Pressure ◽  
Resistance Plasmid
Sign in / Sign up

Export Citation Format

Share Document