Pollen‒pistil interaction in Lilium longiflorum : the role of the pistil in controlling pollen tube growth following cross- and self-pollinations

1982 ◽  
Vol 215 (1198) ◽  
pp. 45-62 ◽  
Keyword(s):  
Pollen Tube ◽  
Pollen Tube Growth ◽  
Active Component ◽  
Lilium Longiflorum ◽  
The Self ◽  
Tube Growth ◽  
Self Incompatibility ◽  
System A ◽  
Pollen Tube Elongation

By cytophysiological methods, the self-incompatibility mechanism of the breeding system in Lilium longiflorum has been examined with particular reference to the synthesis, location and nature of the stylar factors involved in the control of pollen tube development. A ‘bioassay’ has been developed by which the effect of stylar extracts on pollen tube elongation may be investigated. With use of this system, a crude fraction of proteins from the stylar fluid has been shown to inhibit pollen tube growth only when protein fractions from ‘self’ styles are used. The proteins of this fraction have been analysed by thin-layer gel electrofocusing. Changes in the profiles thus obtained following selfing and a heat treatment known to inactivate the self-incompatibility response indicate a highly polarized glycoprotein to be an active component of the system. The various ways by which such a glycoprotein could control pollen tube elongation are considered in detail, and these events in Lilium are discussed in the light of our knowledge of other self-incompatibility systems operating in angiosperms.

scholarly journals Treatment of isolated pistils with protease inhibitors overcomes the self-incompatibility response in buckwheat

2007 ◽  
Vol 59 (1) ◽  
pp. 45-49 ◽  
Author(s):  
Jovanka Miljus-Djukic ◽  
Svetlana Radovic ◽  
Vesna Maksimovic
Keyword(s):  
Pollen Tube ◽  
Protease Inhibitors ◽  
Pollen Tube Growth ◽  
Fluorescence Microscope ◽  
Fagopyrum Esculentum ◽  
The Self ◽  
Tube Growth ◽  
Self Incompatibility ◽  
Fagopyrum Esculentum Moench ◽  
Pepstatin A

Isolated pistils of distylous buckwheat (Fagopyrum esculentum Moench) were treated with protease inhibitors (PMSF, pepstatin A, and antipain). Pistils were cross- or self- pollinated, and growth of pollen tubes was observed under a fluorescence microscope. Treatments with all inhibitors suppressed inhibition of self-pollen tube growth, suggesting that activity of proteases is involved in rejection of self-pollen during the SI response.

scholarly journals Seasonal Changes in the Self-Incompatibility and Pollen Tube Growth in Japanese Pears (Pyrus serotina Rehd.)

1985 ◽  
Vol 53 (4) ◽  
pp. 377-382 ◽  
Author(s):  
Shin HIRATSUKA ◽  
Makoto HIROTA ◽  
Eikichi TAKAHASHI ◽  
Naomi HIRATA
Keyword(s):  
Pollen Tube ◽  
Pollen Tube Growth ◽  
Seasonal Changes ◽  
The Self ◽  
Tube Growth ◽  
Self Incompatibility ◽  
Pyrus Serotina

Effect of spermine and cyclohexylamine on in vitro pollen germination and tube growth in Helianthus annuus

2000 ◽  
Vol 80 (2) ◽  
pp. 241-245 ◽  
Author(s):  
Ergü Çetin ◽  
Cansev Yildirim ◽  
Narçin Palavan-Ünsal ◽  
Meral Ünal
Keyword(s):  
Pollen Tube ◽  
Helianthus Annuus ◽  
Pollen Tube Growth ◽  
Pollen Germination ◽  
Germination Percentage ◽  
Tube Growth ◽  
Pollen Tube Elongation ◽  
Relationship Of

Naturally occurring polyamines (PA) are known to play a key role in growth and development of plants and animals. However, the role of these polycations in the development and germination of the pollen grain is not well understood. The effect of different concentrations of spermine (Spm) on pollen tube growth in Helianthus annuus was investigated. Spermine treatments in the 10−7 to 10−5 M range stimulated pollen tube growth starting in the first 15 min of the incubation period, while 10−4 M Spm treatment resulted in inhibition of pollen tube elongation. The effect of cyclohexylamine (CHA), an inhibitor of Spm synthesis on pollen tube growth and germination percentage was also studied. Cyclohexylamine in the 0.5 × 10−3 M to the 1.5 × 10−3 M range inhibited pollen tube elongation. The relationship of B deficiency, excess B and PA effect on pollen germination and pollen tube growth were also investigated. Key words: Polyamines, pollen, cyclohexylamine, boron

scholarly journals Testing of self-(in)compatibility in apricot cultivars from European breeding programmes

2013 ◽  
Vol 40 (No. 2) ◽  
pp. 65-71 ◽  
Author(s):  
D. Milatović ◽  
D. Nikolić ◽  
B. Krška
Keyword(s):  
Fluorescence Microscopy ◽  
Pollen Tube ◽  
Pollen Tube Growth ◽  
Reliable Method ◽  
Pollen Tubes ◽  
Tube Growth ◽  
Self Incompatibility ◽  
Breeding Programmes

Self-(in)compatibility was tested in 40 new apricot cultivars from European breeding programmes. Pollen-tube growth in pistils from laboratory pollinations was analysed using the fluorescence microscopy. Cultivars were considered self-compatible if at least one pollen tube reached the ovary in the majority of pistils. Cultivars were considered self- incompatible if the growth of pollen tubes in the style stopped along with formation of characteristic swellings. Of the examined cultivars, 18 were self-compatible and 22 were self-incompatible. Fluorescence microscopy provides a relatively rapid and reliable method to determine self-incompatibility in apricot cultivars.      

scholarly journals Paving the Way for Fertilization: The Role of the Transmitting Tract

2021 ◽  
Vol 22 (5) ◽  
pp. 2603
Author(s):  
Ana Marta Pereira ◽  
Diana Moreira ◽  
Sílvia Coimbra ◽  
Simona Masiero
Keyword(s):  
Pollen Tube ◽  
Pollen Tube Growth ◽  
Current Knowledge ◽  
Embryo Sac ◽  
Tube Growth ◽  
Signaling Cascades ◽  
Secretory Cells ◽  
Transmitting Tract ◽  
Specialized Tissue

Angiosperm reproduction relies on the precise growth of the pollen tube through different pistil tissues carrying two sperm cells into the ovules’ embryo sac, where they fuse with the egg and the central cell to accomplish double fertilization and ultimately initiate seed development. A network of intrinsic and tightly regulated communication and signaling cascades, which mediate continuous interactions between the pollen tube and the sporophytic and gametophytic female tissues, ensures the fast and meticulous growth of pollen tubes along the pistil, until it reaches the ovule embryo sac. Most of the pollen tube growth occurs in a specialized tissue—the transmitting tract—connecting the stigma, the style, and the ovary. This tissue is composed of highly secretory cells responsible for producing an extensive extracellular matrix. This multifaceted matrix is proposed to support and provide nutrition and adhesion for pollen tube growth and guidance. Insights pertaining to the mechanisms that underlie these processes remain sparse due to the difficulty of accessing and manipulating the female sporophytic tissues enclosed in the pistil. Here, we summarize the current knowledge on this key step of reproduction in flowering plants with special emphasis on the female transmitting tract tissue.

scholarly journals Impact of Manganese on Pollen Germination and Tube Growth in Lily

2021 ◽  
Vol 74 ◽  
Author(s):  
Thomas Sawidis ◽  
Gülriz Baycu ◽  
Elżbieta Weryszko-Chmielewska ◽  
Aneta Sulborska
Keyword(s):  
Pollen Tube ◽  
Pollen Germination ◽  
Surrounding Medium ◽  
Pollen Grains ◽  
Lilium Longiflorum ◽  
Tube Growth ◽  
Low Concentrations ◽  
Main Effect

Abstract In vitro culture of Lilium longiflorum pollen grains was carried out to determine the role of manganese in pollen germination and pollen tube growth. Pollen germination was adversely affected by the presence of manganese (>10 −8 M), whereas low concentrations (10 −12 –10 −10 M) stimulated the process. Manganese caused morphological anomalies during tube growth, characterized by irregular pollen tube thickening and swollen tips. The main effect was the anomalous cell wall formation at the tip, in which the presence of several organelles reduced the number of secretory vesicles. A loose network of fibrillar material and spherical aggregates, mostly in the tip region, was detected, and this material was progressively loosened into the surrounding medium. As a response to potential toxicity, the excess manganese was isolated in vacuoles, which formed an internal barrier against penetration of manganese to the tip area. Elevated manganese concentrations might affect plant reproduction, resulting in anomalies in gamete development. Consequently, the loss in genetic diversity and decreased fruit set ultimately lower yield.

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