Role of hepatocellular regeneration in chlordecone potentiated hepatotoxicity of carbon tetrachloride

The main purpose of this study is to investigate the role of citrus leaf extract in carbon tetrachloride-induced hepatic injury and its potential molecular mechanism. Carbon tetrachloride was used to construct hepatic injury animal model. To this end, rats were randomly divided into 4 groups: control, carbon tetrachloride-treated, and two carbon tetrachloride + citrus leaf extract-treated groups. The results show that citrus leaf extract treatment significantly reversed the effects of carbon tetrachloride on the body weight changes and liver index. Besides, treatment with citrus leaf extract also reduced the levels of serum liver enzymes and oxidative stress in a dose-dependent manner. H&E staining and western blotting suggested that citrus leaf extract could repair liver histological damage by regulating AMPK and Nrf-2.

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The role of capillarization in hepatic failure: Studies in carbon tetrachloride-induced cirrhosis

Hepatology ◽

10.1002/hep.1840140519 ◽

1991 ◽

Vol 14 (5) ◽

pp. 864-874 ◽

Cited By ~ 61

Author(s):

Antonio Martinez-Hernandez ◽

Jose Martinez

Keyword(s):

Carbon Tetrachloride ◽

Hepatic Failure

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The Hepatoprotective Role of Warburgia salutaris and Iso-Mukaadial Acetate on Carbon tetrachloride Intoxicated Rats Model

Current Bioactive Compounds ◽

10.2174/1573407217666210816105252 ◽

2021 ◽

Vol 17 ◽

Author(s):

Gideon Ayeni ◽

Mthokozisi Blessing Cedric Simelane ◽

Shahidul Islam ◽

Ofentse Jacob Pooe

Keyword(s):

Carbon Tetrachloride ◽

Hepatic Injury ◽

Antioxidant Properties ◽

Hepatic Necrosis ◽

Protective Role ◽

Dependent Manner ◽

Sprague Dawley ◽

Sprague Dawley Rats ◽

Isolated Compound

Background: Medicinal plants together with their isolated bioactive compounds are known for their antioxidant properties which constitute therapeutic agents that are routinely employed in the treatment of liver diseases. Aims of the Study: The current study sought to explore the protective role of Warburgia salutaris and its isolated compound, iso-mukaadial acetate against carbon tetrachloride (CCl4)-induced hepatic injury. Methods: Thirty-five male Sprague Dawley rats were divided into seven groups of five animals each and injected with CCl4 to induce hepatic injury. Results: Treatment with the crude extract of W. salutaris and of iso-mukaadial acetate significantly reduced the levels of alkaline phosphatase, alanine and aspartate aminotransaminases, total bilirubin and malondialdehyde in a dose dependent manner, when compared to untreated groups. Liver histology revealed a reduction in hepatic necrosis and inflammation. Conclusion: The current investigation has demonstrated that W. salutaris extract and iso-mukaadial acetate could mitigate the acute liver injury inflicted by a hepatotoxic inducer in rats.

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Alleviation of carbon tetrachloride-induced hepatocellular damage and oxidative stress with a leaf extract of Glyphae brevis (Tiliaceae)

Journal of Basic and Clinical Physiology and Pharmacology ◽

10.1515/jbcpp-2017-0058 ◽

2018 ◽

Vol 29 (6) ◽

pp. 609-619 ◽

Cited By ~ 1

Author(s):

Lucky Legbosi Nwidu ◽

Yibala I. Oboma ◽

Ekramy Elmorsy ◽

Wayne Grant Carter

Keyword(s):

Carbon Tetrachloride ◽

Leaf Extract ◽

Protective Role ◽

Lipid Peroxidation Product ◽

Hematological Indices ◽

Hepatocellular Damage ◽

Antioxidant Parameters ◽

Mechanistic Basis ◽

Histopathological Studies

Abstract Background Glyphae brevis leaf is reported in ethnomedicine as a treatment for hepatitis and jaundice; however, no studies have hitherto investigated the mechanistic basis of these claims. Methods A hepato-protective role of G. brevis hydromethanolic (GBH) leaf extract was established against carbon tetrachloride (CCl4)-induced hepatotoxicity. Twenty-four hours after a CCl4 challenge, rats were sacrificed and serum hematological indices, lipid profile, and biochemical parameters were determined. The antioxidant enzymes parameters (glutathione, catalase, and superoxide dismutase) and lipid peroxidation product (thiobarbituric reactive substances) levels in liver homogenates were evaluated. Changes in the liver cyto-architecture of different treatment groups were also investigated. Results The GBH extract produced no significant impact on weight and hematological indices. Intoxication with CCl4 significantly (p<0.001–0.05) increased total cholesterol (TC) and low-density lipoproteins (LDL) compared with control rats. Pretreatment with GBH leaf extract significantly reduced triglycerides, TC, and LDL to approaching control levels (p<0.001–0.05). The GBH leaf extract significantly alleviated CCl4-induced elevation of alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase, and the CCl4-induced depression of total protein, and albumin. Liver antioxidant parameters were significantly increased in plant extract-treated rats, and this antagonized the pro-oxidant effect of CCl4. Histopathological studies also supported a hepato-protective effect of GBH. Collectively, the GBH leaf extract alleviated the CCl4-induced hepatotoxicity through improvement of innate antioxidant enzyme levels and lipid metabolism and stabilized the hepatocyte cyto-architecture of intoxicated rats. Conclusions This study establishes the ethnomedicinal role of G. brevis leaf in hepatitis and the mechanistic basis of hepato-protection against CCl4-induced hepatotoxicity.

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