Characterization of two distinct alpha-adrenoceptor binding sites in smooth muscle cell membranes from rat and bovine aorta

1985 ◽  
Vol 329 (3) ◽  
pp. 282-288 ◽  
Author(s):  
Jean-Jacques Descombes ◽  
Jean-Claude Stoclet
Keyword(s):  
Smooth Muscle ◽  
Smooth Muscle Cell ◽  
Muscle Cell ◽  
Binding Sites ◽  
Cell Membranes ◽  
Alpha Adrenoceptor

Isolation and characterization of smooth muscle cell membranes

1974 ◽  
Vol 332 (2) ◽  
pp. 221-232 ◽  
Author(s):  
Mecia M. Oliveira ◽  
Simonte Holzhacker
Keyword(s):  
Smooth Muscle ◽  
Smooth Muscle Cell ◽  
Muscle Cell ◽  
Cell Membranes ◽  
Isolation And Characterization

Characterization of kinin binding sites: identity of B2 receptors in the epithelium and the smooth muscle of the guinea pig ileum

1991 ◽  
Vol 69 (6) ◽  
pp. 818-825 ◽  
Author(s):  
C. Tousignant ◽  
G. Guillemette ◽  
J. Barabé ◽  
N.-E. Rhaleb ◽  
D. Regoli
Keyword(s):  
Smooth Muscle ◽  
Epithelial Cell ◽  
Guinea Pig ◽  
Smooth Muscle Cell ◽  
Muscle Cell ◽  
Binding Sites ◽  
Cell Membranes ◽  
Specific Binding ◽  
Scatchard Analysis ◽  
Guinea Pig Ileum

Binding of [125I-Tyr8]bradykinin (BK) was measured in homogenates of epithelial and smooth muscle layers of the guinea pig ileum. Binding assays were performed at 4 °C for 40 min (smooth muscle) or 90 min (epithelium) in 25 mM PIPES buffer at pH 6.8 in the presence of 1 mM 1,10-phenanthroline, 140 μg/mL bacitracin, 1 mM captopril, 1 mM dithiothreitol, and 0.1% bovine serum albumin. Specific binding of [125I-Tyr8]BK (0.32 nM) to epithelial and smooth muscle cell membranes was linearly related to protein concentration between 0.05 and 0.5 mg/mL. Equilibrium experiments showed that specific binding of [125I-Tyr8]BK was saturable and Scatchard analysis indicated the presence of a high affinity site with a Kd value of 1.6 nM and a Bmax. of 156 fmol/mg of protein in the epithelial cell membranes. In smooth muscle membranes, Kd was 1.8 nM and the maximum number of binding sites was 58 fmol/mg of protein. Unlabelled peptides, namely bradykinin, [Tyr8]BK, [Hyp3]BK, D-Arg[Hyp3]BK, [Hyp3,Tyr(Me8)]BK, and kallidin displaced [125I-Tyr8]BK binding while other peptides, angiotensin II and substance P, had no effect. A series of B2-receptor antagonists displaced [125I-Tyr8]BK from specific binding sites with IC50 values ranging from 16 to 152 nM on epithelial cell membranes; similar values were obtained from smooth muscle cell membranes. These findings suggest that the binding sites in both preparations are of the B2 type. B1-receptor agonists and antagonists were found to be inactive at concentrations up to 10−4 M. Results obtained in the two preparations were compared and a positive highly significant correlation was demonstrated between the two sets of data. It is therefore concluded that B2 receptors found in the epithelium and the smooth muscle membranes of the guinea pig ileum are identical. These sites are similar to the functional receptor mediating the myotropic effect of bradykinin in the ileum.Key words: kinins, epithelium, smooth muscle, guinea pig ileum, binding and biological assays.

Characterization of cholesteryl ester-loaded human coronary vascular smooth muscle cell secretome. A source of potential biomarkers of coronary artery disease

2021 ◽  
Vol 331 ◽  
pp. e67-e68
Author(s):  
J.E.G. Rodríguez ◽  
A. Benitez Amaro ◽  
L. Claudi ◽  
J. Ni ◽  
C. Pallara ◽  
...  
Keyword(s):  
Coronary Artery Disease ◽  
Smooth Muscle ◽  
Coronary Artery ◽  
Vascular Smooth Muscle ◽  
Cholesteryl Ester ◽  
Smooth Muscle Cell ◽  
Muscle Cell ◽  
Artery Disease ◽  
Potential Biomarkers

Characterization of a continuous smooth muscle cell line derived from rabbit aorta

1989 ◽  
Vol 25 (10) ◽  
pp. 892-898 ◽  
Author(s):  
Maurice Nachtigal ◽  
Madan L. Nagpal ◽  
Phillip Greenspan ◽  
Sidonia A. Nachtigal ◽  
Alain Legrand
Keyword(s):  
Smooth Muscle ◽  
Smooth Muscle Cell ◽  
Cell Line ◽  
Muscle Cell ◽  
Rabbit Aorta ◽  
Muscle Cell Line

scholarly journals Erratum: Lazar-Karsten, P., et al. Generation and Characterization of Vascular Smooth Muscle Cell Lines Derived from a Patient with a Bicuspid Aortic Valve. Cells 2016, 5, 19.

Cells ◽  
2016 ◽  
Vol 5 (3) ◽  
pp. 36
Author(s):  
Pamela Lazar-Karsten ◽  
Gazanfer Belge ◽  
Detlev Schult-Badusche ◽  
Tim Focken ◽  
Arlo Radtke ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Aortic Valve ◽  
Vascular Smooth Muscle ◽  
Smooth Muscle Cell ◽  
Cell Lines ◽  
Muscle Cell ◽  
Vascular Smooth Muscle Cell ◽  
Bicuspid Aortic Valve

Growing and differentiating characterization of aortic smooth muscle cell line, p53LMAC01 obtained from p53 knock out mice

1999 ◽  
pp. 99-104
Author(s):  
Tsuyoshi Masuda ◽  
Kazuhiro Ohmi ◽  
Hideki Yamaguchi ◽  
Kazuhide Hasegawa ◽  
Tomoyasu Sugiyama ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Smooth Muscle Cell ◽  
Cell Line ◽  
Muscle Cell ◽  
Aortic Smooth Muscle Cell ◽  
Knock Out ◽  
Aortic Smooth Muscle ◽  
Knock Out Mice ◽  
Muscle Cell Line

Characterization of Clonal Vascular Smooth Muscle Cell Lines Derived from Young and Old Fischer 344 Rats

2011 ◽  
Vol 47 (7) ◽  
pp. 445-450 ◽  
Author(s):  
William E. Schutzer ◽  
Douglas R. Beard ◽  
John F. Reed ◽  
Scott L. Mader
Keyword(s):  
Smooth Muscle ◽  
Vascular Smooth Muscle ◽  
Smooth Muscle Cell ◽  
Cell Lines ◽  
Muscle Cell ◽  
Vascular Smooth Muscle Cell ◽  
Fischer 344 Rats ◽  
Fischer 344
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