Demonstration of new Ca2+ binding sites by vesicular fraction of smooth muscle cell plasma membranes in the presence of alamethicin

1990 ◽  
Vol 109 (2) ◽  
pp. 125-127
Author(s):  
M. E. Kucherenko ◽  
V. K. Rybal'chenko
Keyword(s):  
Smooth Muscle ◽  
Smooth Muscle Cell ◽  
Muscle Cell ◽  
Binding Sites ◽  
Plasma Membranes ◽  
Cell Plasma

Inhomogeneous distribution of filipin–sterol complexes in smooth muscle cell plasma membrane

Nature ◽  
1979 ◽  
Vol 280 (5720) ◽  
pp. 328-329 ◽  
Author(s):  
ROBERTO MONTESANO
Keyword(s):  
Smooth Muscle ◽  
Plasma Membrane ◽  
Smooth Muscle Cell ◽  
Muscle Cell ◽  
Inhomogeneous Distribution ◽  
Cell Plasma Membrane ◽  
Cell Plasma

Characterization of kinin binding sites: identity of B2 receptors in the epithelium and the smooth muscle of the guinea pig ileum

1991 ◽  
Vol 69 (6) ◽  
pp. 818-825 ◽  
Author(s):  
C. Tousignant ◽  
G. Guillemette ◽  
J. Barabé ◽  
N.-E. Rhaleb ◽  
D. Regoli
Keyword(s):  
Smooth Muscle ◽  
Epithelial Cell ◽  
Guinea Pig ◽  
Smooth Muscle Cell ◽  
Muscle Cell ◽  
Binding Sites ◽  
Cell Membranes ◽  
Specific Binding ◽  
Scatchard Analysis ◽  
Guinea Pig Ileum

Binding of [125I-Tyr8]bradykinin (BK) was measured in homogenates of epithelial and smooth muscle layers of the guinea pig ileum. Binding assays were performed at 4 °C for 40 min (smooth muscle) or 90 min (epithelium) in 25 mM PIPES buffer at pH 6.8 in the presence of 1 mM 1,10-phenanthroline, 140 μg/mL bacitracin, 1 mM captopril, 1 mM dithiothreitol, and 0.1% bovine serum albumin. Specific binding of [125I-Tyr8]BK (0.32 nM) to epithelial and smooth muscle cell membranes was linearly related to protein concentration between 0.05 and 0.5 mg/mL. Equilibrium experiments showed that specific binding of [125I-Tyr8]BK was saturable and Scatchard analysis indicated the presence of a high affinity site with a Kd value of 1.6 nM and a Bmax. of 156 fmol/mg of protein in the epithelial cell membranes. In smooth muscle membranes, Kd was 1.8 nM and the maximum number of binding sites was 58 fmol/mg of protein. Unlabelled peptides, namely bradykinin, [Tyr8]BK, [Hyp3]BK, D-Arg[Hyp3]BK, [Hyp3,Tyr(Me8)]BK, and kallidin displaced [125I-Tyr8]BK binding while other peptides, angiotensin II and substance P, had no effect. A series of B2-receptor antagonists displaced [125I-Tyr8]BK from specific binding sites with IC50 values ranging from 16 to 152 nM on epithelial cell membranes; similar values were obtained from smooth muscle cell membranes. These findings suggest that the binding sites in both preparations are of the B2 type. B1-receptor agonists and antagonists were found to be inactive at concentrations up to 10−4 M. Results obtained in the two preparations were compared and a positive highly significant correlation was demonstrated between the two sets of data. It is therefore concluded that B2 receptors found in the epithelium and the smooth muscle membranes of the guinea pig ileum are identical. These sites are similar to the functional receptor mediating the myotropic effect of bradykinin in the ileum.Key words: kinins, epithelium, smooth muscle, guinea pig ileum, binding and biological assays.

scholarly journals Hydrogen sulfide dilates cerebral arterioles by activating smooth muscle cell plasma membrane K ATP channels

2011 ◽  
Vol 25 (S1) ◽  
Author(s):  
Guohua Liang ◽  
Adebowale Adebiyi ◽  
Elizabeth M. McNally ◽  
Charles W. Leffler ◽  
Jonathan H. Jaggar
Keyword(s):  
Smooth Muscle ◽  
Plasma Membrane ◽  
Hydrogen Sulfide ◽  
Smooth Muscle Cell ◽  
Muscle Cell ◽  
Cell Plasma Membrane ◽  
Cell Plasma ◽  
Cerebral Arterioles

Interaction between smooth muscle cell plasma membrane and a flat lipid membrane

1985 ◽  
Vol 100 (4) ◽  
pp. 1454-1457
Author(s):  
V. K. Rybal'chenko ◽  
V. I. Karamushka
Keyword(s):  
Smooth Muscle ◽  
Plasma Membrane ◽  
Smooth Muscle Cell ◽  
Muscle Cell ◽  
Lipid Membrane ◽  
Cell Plasma Membrane ◽  
Cell Plasma

1429: Upregulation of Rho-Kinase in a Human Corpus Cavernosum Smooth Muscle Cell Line in Response to Hypoxia

2004 ◽  
Vol 171 (4S) ◽  
pp. 376-377
Author(s):  
Yongmu Zheng ◽  
Shaohua Chang ◽  
Alan J. Wein ◽  
Samuel Chacko ◽  
Michael E. DiSanto
Keyword(s):  
Smooth Muscle ◽  
Smooth Muscle Cell ◽  
Cell Line ◽  
Muscle Cell ◽  
Corpus Cavernosum ◽  
Rho Kinase ◽  
Muscle Cell Line
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