Restoration of peroxisome biogenesis in a peroxisome-deficient mammalian cell line by expression of either the 35 kDa or the 70 kDa peroxisomal membrane proteins

1994 ◽  
Vol 17 (3) ◽  
pp. 327-329 ◽  
Author(s):  
J. G�rtner ◽  
C. Obie ◽  
P. Watkins ◽  
D. Valle
Keyword(s):  
Membrane Proteins ◽  
Cell Line ◽  
Mammalian Cell ◽  
Mammalian Cell Line ◽  
Peroxisome Biogenesis ◽  
Peroxisomal Membrane

Pharmacology of human dopamine D3 receptor expressed in a mammalian cell line: comparison with D2 receptor

1992 ◽  
Vol 225 (4) ◽  
pp. 331-337 ◽  
Author(s):  
Pierre Sokoloff ◽  
Marc Andrieux ◽  
Roger Besançon ◽  
Catherine Pilon ◽  
Marie-Pascale Martres ◽  
...  
Keyword(s):  
Cell Line ◽  
Mammalian Cell ◽  
D2 Receptor ◽  
Dopamine D3 Receptor ◽  
D3 Receptor ◽  
Mammalian Cell Line ◽  
Dopamine D3

Instability of simple sequence repeats in a mammalian cell line

1994 ◽  
Vol 3 (2) ◽  
pp. 253-256 ◽  
Author(s):  
Rosann A. Farber ◽  
Thomas D. Petes ◽  
Margaret Dominska ◽  
Sarah S. Hudgens ◽  
R.Michael Liskay
Keyword(s):  
Cell Line ◽  
Mammalian Cell ◽  
Simple Sequence Repeats ◽  
Mammalian Cell Line ◽  
Simple Sequence

Radiosensitization of a Mammalian Cell Line with 5-Bromodeoxyuridine

1962 ◽  
Vol 17 (4) ◽  
pp. 479 ◽  
Author(s):  
N. Delihas ◽  
M. A. Rich ◽  
M. L. Eidinoff
Keyword(s):  
Cell Line ◽  
Mammalian Cell ◽  
Mammalian Cell Line

scholarly journals Construction and Modelling of an Inducible Positive Feedback Loop Stably Integrated in a Mammalian Cell-Line

2011 ◽  
Vol 7 (6) ◽  
pp. e1002074 ◽  
Author(s):  
Velia Siciliano ◽  
Filippo Menolascina ◽  
Lucia Marucci ◽  
Chiara Fracassi ◽  
Immacolata Garzilli ◽  
...  
Keyword(s):  
Cell Line ◽  
Mammalian Cell ◽  
Positive Feedback ◽  
Feedback Loop ◽  
Mammalian Cell Line ◽  
Positive Feedback Loop

The use of ‘Omics technology to rationally improve industrial mammalian cell line performance

2015 ◽  
Vol 113 (1) ◽  
pp. 26-38 ◽  
Author(s):  
Amanda M. Lewis ◽  
Nicholas R. Abu-Absi ◽  
Michael C. Borys ◽  
Zheng Jian Li
Keyword(s):  
Cell Line ◽  
Mammalian Cell ◽  
Mammalian Cell Line ◽  
Omics Technology

scholarly journals Expression of Recombinant Non Structural 1 Protein of Dengue Virus Serotype-2 in Mammalian Cell Line

2019 ◽  
Vol 13 (1) ◽  
pp. 36-42 ◽  
Author(s):  
FITHRIYAH SJATHA ◽  
◽  
OKTIVIA CHANDRA MUSTIKA ◽  
ANGKY BUDIANTI ◽  
TJAHJANI MIRAWATI SUDIRO ◽  
...  
Keyword(s):  
Dengue Virus ◽  
Cell Line ◽  
Mammalian Cell ◽  
Mammalian Cell Line ◽  
Virus Serotype ◽  
Dengue Virus Serotype 2

Biological Studies of a Baculovirus in a Mammalian Cell Line

Intervirology ◽  
1980 ◽  
Vol 13 (6) ◽  
pp. 331-341 ◽  
Author(s):  
Arthur H. Mclntosh ◽  
Rebecca Shamy
Keyword(s):  
Cell Line ◽  
Mammalian Cell ◽  
Mammalian Cell Line ◽  
Biological Studies

Peroxisomal membrane proteins are properly targeted to peroxisomes in the absence of COPI- and COPII-mediated vesicular transport

2001 ◽  
Vol 114 (11) ◽  
pp. 2199-2204 ◽  
Author(s):  
Tineke Voorn-Brouwer ◽  
Astrid Kragt ◽  
Henk F. Tabak ◽  
Ben Distel
Keyword(s):  
Endoplasmic Reticulum ◽  
Membrane Proteins ◽  
Secretory Pathway ◽  
Human Fibroblasts ◽  
Vesicle Transport ◽  
Peroxisome Biogenesis ◽  
Peroxisomal Membrane ◽  
Classic Model ◽  
Early Secretory Pathway

The classic model for peroxisome biogenesis states that new peroxisomes arise by the fission of pre-existing ones and that peroxisomal matrix and membrane proteins are recruited directly from the cytosol. Recent studies challenge this model and suggest that some peroxisomal membrane proteins might traffic via the endoplasmic reticulum to peroxisomes. We have studied the trafficking in human fibroblasts of three peroxisomal membrane proteins, Pex2p, Pex3p and Pex16p, all of which have been suggested to transit the endoplasmic reticulum before arriving in peroxisomes. Here, we show that targeting of these peroxisomal membrane proteins is not affected by inhibitors of COPI and COPII that block vesicle transport in the early secretory pathway. Moreover, we have obtained no evidence for the presence of these peroxisomal membrane proteins in compartments other than peroxisomes and demonstrate that COPI and COPII inhibitors do not affect peroxisome morphology or integrity. Together, these data fail to provide any evidence for a role of the endoplasmic reticulum in peroxisome biogenesis.

A mammalian cell line deficient in activity of the DNA repair enzyme 5-hydroxymethyluracil-DNA glycosylase is resistant to the toxic effects of the thymidine analog 5-hydroxymethyl-2'-deoxyuridine

1992 ◽  
Vol 12 (12) ◽  
pp. 5536-5540
Author(s):  
R J Boorstein ◽  
L N Chiu ◽  
G W Teebor
Keyword(s):  
Dna Repair ◽  
Cell Line ◽  
Mammalian Cell ◽  
Dna Glycosylase ◽  
Mammalian Cell Line ◽  
Repair Enzyme ◽  
Thymidine Analog ◽  
Large Numbers ◽  
Mechanism Of Toxicity ◽  
Base Excision

We isolated a mutant mammalian cell line lacking activity for the DNA repair enzyme 5-hydroxymethyluracil-DNA glycosylase (HmUra-DNA glycosylase). The mutant was isolated through its resistance to the thymidine analog 5-hydroxymethyl-2'-deoxyuridine (HmdUrd). The mutant incorporates HmdUrd into DNA to the same extent as the parent line but, lacking the repair enzyme, does not remove it. The phenotype of the mutant demonstrates that the toxicity of HmdUrd does not result from substitution of thymine in DNA by HmUra but rather from the removal via base excision of large numbers of HmUra residues in DNA. This finding elucidates a novel mechanism of toxicity for a xenobiotic nucleoside. Furthermore, the isolation of this line supports our hypothesis that the enzymatic repairability of HmUra derives not from its formation opposite adenine via the oxidation of thymine, but rather from its formation opposite guanine as a product of the oxidation and subsequent deamination of 5-methylcytosine.

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