Wound healing and regeneration in the imaginal wing disc ofDrosophila

1981 ◽  
Vol 190 (4) ◽  
pp. 185-190 ◽  
Author(s):  
Leslie Dale ◽  
Mary Bownes
Keyword(s):  
Wound Healing ◽  
Wing Disc ◽  
Imaginal Wing Disc

Pattern regulation in fragments of Drosophila wing discs which show variable wound healing

Development ◽  
1985 ◽  
Vol 85 (1) ◽  
pp. 95-109
Author(s):  
Leslie Dale ◽  
Mary Bownes
Keyword(s):  
Cell Proliferation ◽  
Wound Healing ◽  
Wing Disc ◽  
Wound Edge ◽  
Drosophila Wing ◽  
Imaginal Wing Disc ◽  
Wing Discs ◽  
Pattern Regulation ◽  
Mode Of Regulation

When complementary fragments of the imaginal wing disc of Drosophila are cultured for several days prior to inducing metamorphosis, usually one fragment will regenerate while the second duplicates. It has been proposed that wound healing plays an important part in disc regulation by initiating cell proliferation and determining the mode of regulation (regeneration/duplication). To test the latter proposal 15 types of wing disc fragments were examined for variability both in the mode of wound healing and the mode of pattern regulation. Two modes of wound healing were observed, regular—the two wound edges heal with each other, and irregular—each wound edge heals with itself. When cultured separately fragments that healed regularly regenerated, while fragments that healed irregularly duplicated. This suggests that the mode of wound healing determines the mode of pattern regulation.

Dpp signalling is a key effector of the wing-body wall subdivision of theDrosophilamesothorax

Development ◽  
2002 ◽  
Vol 129 (16) ◽  
pp. 3815-3823 ◽  
Author(s):  
Florencia Cavodeassi ◽  
Isabel Rodríguez ◽  
Juan Modolell
Keyword(s):  
Body Wall ◽  
Larval Instar ◽  
Proximal Part ◽  
Distal Region ◽  
Wing Disc ◽  
Signalling Pathway ◽  
Wing Development ◽  
C Cells ◽  
Imaginal Wing Disc ◽  
Anterior Posterior

During development, the imaginal wing disc of Drosophila is subdivided along the proximal-distal axis into different territories that will give rise to body wall (notum and mesothoracic pleura) and appendage (wing hinge and wing blade). Expression of the Iroquois complex (Iro-C) homeobox genes in the most proximal part of the disc defines the notum, since Iro-C– cells within this territory acquire the identity of the adjacent distal region, the wing hinge. Here we analyze how the expression of Iro-C is confined to the notum territory. Neither Wingless signalling, which is essential for wing development, nor Vein-dependent EGFR signalling, which is needed to activate Iro-C, appear to delimit Iro-C expression. We show that a main effector of this confinement is the TGFβ homolog Decapentaplegic (Dpp), a molecule known to pattern the disc along its anterior-posterior axis. At early second larval instar, the Dpp signalling pathway functions only in the wing and hinge territories, represses Iro-C and confines its expression to the notum territory. Later, Dpp becomes expressed in the most proximal part of the notum and turns off Iro-C in this region. This downregulation is associated with the subdivision of the notum into medial and lateral regions.

scholarly journals Junctional and cytoplasmic contributions in wound healing

2020 ◽  
Vol 17 (169) ◽  
pp. 20200264 ◽  
Author(s):  
Payman Mosaffa ◽  
Robert J. Tetley ◽  
Antonio Rodríguez-Ferran ◽  
Yanlan Mao ◽  
José J. Muñoz
Keyword(s):  
Wound Healing ◽  
Viscoelastic Properties ◽  
Healing Process ◽  
Wing Disc ◽  
Wound Healing Process ◽  
Minor Effect ◽  
Closure Rate ◽  
A Minor ◽  
Contractile Forces ◽  
Viscoelastic Rheology

Wound healing is characterized by the re-epitheliation of a tissue through the activation of contractile forces concentrated mainly at the wound edge. While the formation of an actin purse string has been identified as one of the main mechanisms, far less is known about the effects of the viscoelastic properties of the surrounding cells, and the different contribution of the junctional and cytoplasmic contractilities. In this paper, we simulate the wound healing process, resorting to a hybrid vertex model that includes cell boundary and cytoplasmic contractilities explicitly, together with a differentiated viscoelastic rheology based on an adaptive rest-length. From experimental measurements of the recoil and closure phases of wounds in the Drosophila wing disc epithelium, we fit tissue viscoelastic properties. We then analyse in terms of closure rate and energy requirements the contributions of junctional and cytoplasmic contractilities. Our results suggest that reduction of junctional stiffness rather than cytoplasmic stiffness has a more pronounced effect on shortening closure times, and that intercalation rate has a minor effect on the stored energy, but contributes significantly to shortening the healing duration, mostly in the later stages.

Connecting Hh, Dpp and EGF signalling in patterning of theDrosophilawing; the pivotal role ofcollier/knotin the AP organiser

Development ◽  
2002 ◽  
Vol 129 (18) ◽  
pp. 4261-4269 ◽  
Author(s):  
Michèle Crozatier ◽  
Bruno Glise ◽  
Alain Vincent
Keyword(s):  
Cell Proliferation ◽  
Central Region ◽  
Wing Disc ◽  
Signalling Pathways ◽  
Wild Type ◽  
Drosophila Wing ◽  
Egfr Ligand ◽  
Imaginal Wing Disc ◽  
Primary Determinant ◽  
Dose Dependent

Hedgehog (Hh) signalling from posterior (P) to anterior (A) cells is the primary determinant of AP polarity in the limb field in insects and vertebrates. Hh acts in part by inducing expression of Decapentaplegic (Dpp), but how Hh and Dpp together pattern the central region of the Drosophila wing remains largely unknown. We have re-examined the role played by Collier (Col), a dose-dependent Hh target activated in cells along the AP boundary, the AP organiser in the imaginal wing disc. We found that col mutant wings are smaller than wild type and lack L4 vein, in addition to missing the L3-L4 intervein and mis-positioning of the anterior L3 vein. We link these phenotypes to col requirement for the local upregulation of both emc and N, two genes involved in the control of cell proliferation, the EGFR ligand Vein and the intervein determination gene blistered. We further show that attenuation of Dpp signalling in the AP organiser is also col dependent and, in conjunction with Vein upregulation, required for formation of L4 vein. A model recapitulating the molecular interplay between the Hh, Dpp and EGF signalling pathways in the wing AP organiser is presented.

Transdetermination in imaginal wing disc fragments of Drosophila melanogaster

1983 ◽  
Vol 97 (1) ◽  
pp. 19-26 ◽  
Author(s):  
Brooke S. Kirby ◽  
Peter J. Bryant ◽  
Howard A. Schneiderman
Keyword(s):  
Drosophila Melanogaster ◽  
Wing Disc ◽  
Imaginal Wing Disc

Localized cell death in Drosophila imaginal wing disc epithelium caused by the mutation apterous-blot

1984 ◽  
Vol 104 (2) ◽  
pp. 489-496 ◽  
Author(s):  
Bonnie Joy Sedlak ◽  
René Manzo ◽  
Mary Stevens
Keyword(s):  
Cell Death ◽  
Wing Disc ◽  
Imaginal Wing Disc
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