Dpp signalling is a key effector of the wing-body wall subdivision of theDrosophilamesothorax

Development ◽  
2002 ◽  
Vol 129 (16) ◽  
pp. 3815-3823 ◽  
Author(s):  
Florencia Cavodeassi ◽  
Isabel Rodríguez ◽  
Juan Modolell
Keyword(s):  
Body Wall ◽  
Larval Instar ◽  
Proximal Part ◽  
Distal Region ◽  
Wing Disc ◽  
Signalling Pathway ◽  
Wing Development ◽  
C Cells ◽  
Imaginal Wing Disc ◽  
Anterior Posterior

During development, the imaginal wing disc of Drosophila is subdivided along the proximal-distal axis into different territories that will give rise to body wall (notum and mesothoracic pleura) and appendage (wing hinge and wing blade). Expression of the Iroquois complex (Iro-C) homeobox genes in the most proximal part of the disc defines the notum, since Iro-C– cells within this territory acquire the identity of the adjacent distal region, the wing hinge. Here we analyze how the expression of Iro-C is confined to the notum territory. Neither Wingless signalling, which is essential for wing development, nor Vein-dependent EGFR signalling, which is needed to activate Iro-C, appear to delimit Iro-C expression. We show that a main effector of this confinement is the TGFβ homolog Decapentaplegic (Dpp), a molecule known to pattern the disc along its anterior-posterior axis. At early second larval instar, the Dpp signalling pathway functions only in the wing and hinge territories, represses Iro-C and confines its expression to the notum territory. Later, Dpp becomes expressed in the most proximal part of the notum and turns off Iro-C in this region. This downregulation is associated with the subdivision of the notum into medial and lateral regions.

Repression of Teashirt marks the initiation of wing development

Development ◽  
2002 ◽  
Vol 129 (10) ◽  
pp. 2411-2418 ◽  
Author(s):  
Jun Wu ◽  
Stephen M. Cohen
Keyword(s):  
Body Wall ◽  
Larval Instar ◽  
Wing Disc ◽  
Wing Development ◽  
Homeodomain Protein ◽  
Zinc Finger Transcription Factor ◽  
Adult Wing ◽  
Combined Action ◽  
Wing Pouch ◽  
Early Activity

The wing imaginal disc comprises the primordia of the adult wing and the dorsal thoracic body wall. During second larval instar, the wing disc is subdivided into distinct domains that correspond to the presumptive wing and body wall. Early activity of the signaling protein Wingless has been implicated in the specification of the wing primordium. Wingless mutants can produce animals in which the wing is replaced by a duplication of thoracic structures. Specification of wing fate has been visualized by expression of the POU-homeodomain protein Nubbin in the presumptive wing territory and by repression of the homeodomain protein Homothorax. We report that repression of the zinc-finger transcription factor Teashirt (Tsh) is the earliest event in wing specification. Repression of Tsh by the combined action of Wingless and Decapentaplegic is required for wing pouch formation and for subsequent repression of Hth. Thus, repression of Tsh defines the presumptive wing earlier in development than repression of Hth, which must therefore be considered a secondary event.

patched overexpression alters wing disc size and pattern: transcriptional and post-transcriptional effects on hedgehog targets

Development ◽  
1995 ◽  
Vol 121 (12) ◽  
pp. 4161-4170 ◽  
Author(s):  
R.L. Johnson ◽  
J.K. Grenier ◽  
M.P. Scott
Keyword(s):  
Protein Kinase ◽  
Protein Kinase A ◽  
Target Genes ◽  
Critical Role ◽  
Wing Disc ◽  
Wing Vein ◽  
Anterior Compartment ◽  
Protein Levels ◽  
Anterior Posterior ◽  
Kinase A

The membrane protein, Patched, plays a critical role in patterning embryonic and imaginal tissues in Drosophila. patched constitutively inactivates the transcription of target genes such as wingless, decapentaplegic, and patched itself. The secreted protein, Hedgehog, induces transcription of target genes by opposing the Patched signaling pathway. Using the Gal4 UAS system we have overexpressed patched in wing imaginal discs and found that high Patched levels, expressed in either normal or ectopic patterns, result in loss of wing vein patterning in both compartments centering at the anterior/posterior border. In addition, patched inhibits the formation of the mechanosensory neurons, the campaniform sensilla, in the wing blade. The patched wing vein phenotype is modulated by mutations in hedgehog and cubitus interruptus (ci). Patched overexpression inhibits transcription of patched and decapentaplegic and post-transcriptionally decreases the amount of Ci protein at the anterior/posterior boundary. In hedgehogMrt wing discs, which express ectopic hedgehog, Ci levels are correspondingly elevated, suggesting that hedgehog relieves patched repression of Ci accumulation. Protein kinase A also regulates Ci; protein kinase A mutant clones in the anterior compartment have increased levels of Ci protein. Thus patched influences wing disc patterning by decreasing Ci protein levels and inactivating hedgehog target genes in the anterior compartment.

scholarly journals Bmmp influences wing shape by regulating anterior-posterior and proximal-distal axis development

2021 ◽  
Author(s):  
Yunlong Zou ◽  
Xin Ding ◽  
Li Zhang ◽  
Lifeng Xu ◽  
Shubo Liang ◽  
...  
Keyword(s):  
Molecular Mapping ◽  
Wing Shape ◽  
Wing Development ◽  
Mrna Levels ◽  
Nucleotide Polymorphisms ◽  
Insect Wing ◽  
Expression Of Genes ◽  
Flight Capacity ◽  
Null Mutants ◽  
Anterior Posterior

Insect wings are subject to strong selective pressure, resulting in the evolution of remarkably diverse wing shapes that largely determine flight capacity. However, the genetic basis and regulatory mechanisms underlying wing shape development are not well understood. The silkworm Bombyx mori micropterous ( mp ) mutant exhibits shortened wing length and enlarged vein spacings , albeit without changes in total wing area. Thus, the mp mutant comprises a valuable genetic resource for studying wing shape development. In this study, we used molecular mapping to identify the gene responsible for the mp phenotype and designated it Bmmp . Phenotype-causing mutations were identified as indels and single nucleotide polymorphisms in non-coding regions. These mutations resulted in decreased Bmmp mRNA levels and changes in transcript isoform composition. Bmmp null mutants were generated by CRISPR/Cas9 and exhibited significantly smaller wings. By examining the expression of genes critical to wing development in wildtype and Bmmp null mutants, we found that Bm mp exerts its function by coordinately modulating anterior-posterior and proximal-distal axis development. We also studied a Drosophila mp mutant and found that Bmmp is functionally conserved in Drosophila . The Drosophila mp mutant strain exhibits curly wings of reduced size and a complete loss of flight capacity. Our results increase our understanding of the mechanisms underpinning insect wing development and reveal potential targets for pest control.

Electrophysiology of Acanthocephalan Body Wall Muscles

1979 ◽  
Vol 82 (1) ◽  
pp. 273-280
Author(s):  
B. S. WONG ◽  
DONALD M. MILLER ◽  
T. T. DUNAGAN
Keyword(s):  
Light Microscopy ◽  
Intracellular Recording ◽  
Body Wall ◽  
Membrane Potentials ◽  
The Body ◽  
Spontaneous Potential ◽  
Body Wall Muscles ◽  
Macracanthorhynchus Hirudinaceus ◽  
Anterior Posterior

Body wall muscles of an acanthocephalan Macracanthorhynchus hirudinaceus were studied by means of scanning and light microscopy and intracellular recording of potentials. Three types of spontaneous potential changes were found: larger (L) potentials which usually exhibited overshoot and were as large as 65 mV; smaller symmetric (A) potentials approximately 15 mV in amplitude; and even smaller asymmetric (S) potentials which sometimes reached 10 mV. The potentials recorded depended upon the position of the electrode in the anterior-posterior, as well as the medialateral, axis. Tetrodotoxin eliminated L but not S potentials. Ouabain lengthened the time for depolarization of L potentials and depolarized the membrane potentials. It is suggested that the rete system activates the body wall muscles in Acanthocephala.

hrs gene and borders of compartments of imaginal wing disc in Drosophila melanogaster

2015 ◽  
Vol 51 (10) ◽  
pp. 1040-1043
Author(s):  
E. V. Marilovtseva ◽  
L. V. Omelyanchuk
Keyword(s):  
Drosophila Melanogaster ◽  
Wing Disc ◽  
Imaginal Wing Disc

Connecting Hh, Dpp and EGF signalling in patterning of theDrosophilawing; the pivotal role ofcollier/knotin the AP organiser

Development ◽  
2002 ◽  
Vol 129 (18) ◽  
pp. 4261-4269 ◽  
Author(s):  
Michèle Crozatier ◽  
Bruno Glise ◽  
Alain Vincent
Keyword(s):  
Cell Proliferation ◽  
Central Region ◽  
Wing Disc ◽  
Signalling Pathways ◽  
Wild Type ◽  
Drosophila Wing ◽  
Egfr Ligand ◽  
Imaginal Wing Disc ◽  
Primary Determinant ◽  
Dose Dependent

Hedgehog (Hh) signalling from posterior (P) to anterior (A) cells is the primary determinant of AP polarity in the limb field in insects and vertebrates. Hh acts in part by inducing expression of Decapentaplegic (Dpp), but how Hh and Dpp together pattern the central region of the Drosophila wing remains largely unknown. We have re-examined the role played by Collier (Col), a dose-dependent Hh target activated in cells along the AP boundary, the AP organiser in the imaginal wing disc. We found that col mutant wings are smaller than wild type and lack L4 vein, in addition to missing the L3-L4 intervein and mis-positioning of the anterior L3 vein. We link these phenotypes to col requirement for the local upregulation of both emc and N, two genes involved in the control of cell proliferation, the EGFR ligand Vein and the intervein determination gene blistered. We further show that attenuation of Dpp signalling in the AP organiser is also col dependent and, in conjunction with Vein upregulation, required for formation of L4 vein. A model recapitulating the molecular interplay between the Hh, Dpp and EGF signalling pathways in the wing AP organiser is presented.

Transdetermination in imaginal wing disc fragments of Drosophila melanogaster

1983 ◽  
Vol 97 (1) ◽  
pp. 19-26 ◽  
Author(s):  
Brooke S. Kirby ◽  
Peter J. Bryant ◽  
Howard A. Schneiderman
Keyword(s):  
Drosophila Melanogaster ◽  
Wing Disc ◽  
Imaginal Wing Disc
Sign in / Sign up

Export Citation Format

Share Document