Enhanced expression of mRNA for insulin-like growth factor-1 in post-burn hypertrophic scar tissue and its fibrogenic role by dermal fibroblasts

1995 ◽  
Vol 148 (1) ◽  
pp. 25-32 ◽  
Author(s):  
Aziz Ghahary ◽  
You J. Shen ◽  
Bernadette Nedelec ◽  
Paul G. Scott ◽  
Edward E. Tredget
Keyword(s):  
Growth Factor ◽  
Hypertrophic Scar ◽  
Scar Tissue ◽  
Dermal Fibroblasts ◽  
Insulin Like Growth Factor ◽  
Enhanced Expression ◽  
Hypertrophic Scar Tissue

Fibroblasts from Post-Burn Hypertrophic Scar Tissue Synthesize Less Decorin than Normal Dermal Fibroblasts

1998 ◽  
Vol 94 (5) ◽  
pp. 541-547 ◽  
Author(s):  
P. G. Scott ◽  
C. M. Dodd ◽  
A. Ghahary ◽  
Y.J. Shen ◽  
E. E. Tredget
Keyword(s):  
Growth Factor ◽  
Hypertrophic Scar ◽  
Transforming Growth Factor ◽  
Scar Tissue ◽  
Transforming Growth Factor Β1 ◽  
Dermal Fibroblasts ◽  
Northern Analysis ◽  
Cell Strains ◽  
Hypertrophic Scar Tissue ◽  
Small Proteoglycan

1. Fibroblast cultures were established from biopsies of hypertrophic scar and normal dermis taken from nine patients recovering from second- and third-degree burns. The capacity of these fibroblasts to synthesize the small proteoglycan decorin was assessed by quantitative Western blot analysis of conditioned medium collected from confluent cultures. Levels of mRNA for decorin were assessed by quantitative Northern analysis. Since transforming growth factor-β1 is implicated in various fibrotic conditions, including post-burn hypertrophic scar, its effect on decorin synthesis by these paired fibroblast cell strains was assessed. 2. Production of decorin was lower in all cell strains of hypertrophic scar fibroblasts tested, compared with normal dermal fibroblasts cultured from the same patients (mean 49 ± 23%; P < 0.001, n = 9). Levels of mRNA for decorin were also lower (mean 59 ± 28%; P < 0.02, n = 7) but those for biglycan and versican were not significantly different. Four pairs of cell strains were examined at more than one passage and the differences in decorin protein were found to be phenotypically persistent. Treatment of confluent cultures with transforming growth factor-β1 for 3 days caused a reduction in both decorin protein and mRNA in all six strains of hypertrophic scar fibroblasts tested and in five of six strains of normal dermal fibroblasts. An increase in the length of the dermatan sulphate chain on decorin, a previously reported characteristic of this glycosaminoglycan in hypertrophic scar, was seen in all but two of the strains treated with transforming growth factor-β1. The depression of decorin synthesis by transforming growth factor-β1 was reversed on removal of the agent and passaging the fibroblasts. 3. The reduced capacity of fibroblasts in hypertrophic scar tissue to synthesize decorin may have implications for the development of the condition since this small proteoglycan is involved in tissue organization and may also play a role in modulating the activity in vivo of fibrogenic cytokines such as transforming growth factor-β1.

scholarly journals Loureirin B Inhibits Hypertrophic Scar Formation via Inhibition of the TGF-β1-ERK/JNK Pathway

2015 ◽  
Vol 37 (2) ◽  
pp. 666-676 ◽  
Author(s):  
Ting He ◽  
Xiaozhi Bai ◽  
Longlong Yang ◽  
Lei Fan ◽  
Yan Li ◽  
...  
Keyword(s):  
Hypertrophic Scar ◽  
Ex Vivo ◽  
Scar Tissue ◽  
Scar Formation ◽  
Jnk Pathway ◽  
Protein Levels ◽  
Mapk Inhibitors ◽  
Hypertrophic Scar Tissue ◽  
Loureirin B ◽  
Tgf Β1

Background/Aims: Our previous study confirmed that Loureirin B (LB) can inhibit hypertrophic scar formation. However, the mechanism of LB-mediated inhibition of scar formation is still unknown. Methods: Immunohistochemistry was used to detect expression of Col1, FN and TGF-β1 in skin and scar tissue. Fibroblasts were stimulated with TGF-β1 to mimic scar formation. LB or MAPK inhibitors were used to study the pathways involved in the process. Western blotting was used to evaluate the expression of p-JNK, p-ERK, p-p38, Col1 and FN. The contractile capacity of fibroblasts was evaluated using a gel contraction assay. Tissues were cultured ex vivo with LB to further investigate the participation of ERK and JNK in the LB-mediated inhibition of scar formation. Results: FN and Col1 were up regulated in hypertrophic scars. LB down regulated p-ERK and p-JNK in TGF-β1-stimulated fibroblasts, while levels of phosphorylated p38 did not change. The down regulation of p-ERK and p-JNK was associated with a reduction of Col1 and FN. Similarly, inhibition of ERK and JNK down regulated the expression of Col1 and FN in TGF-β1-stimulated fibroblasts. LB down regulated protein levels of p-ERK and p-JNK in cultured hypertrophic scar tissue ex vivo. Conclusions: This study suggests that LB can inhibit scar formation through the ERK/JNK pathway.

The Use of Silicone Gel Sheets in the Control of Hypertrophic Scar Tissue

1988 ◽  
Vol 51 (7) ◽  
pp. 248-249 ◽  
Author(s):  
Rosemary Gollop
Keyword(s):  
Retrospective Study ◽  
Hypertrophic Scar ◽  
Scar Tissue ◽  
City Hospital ◽  
Hypertrophic Scarring ◽  
The Past ◽  
Silicone Gel ◽  
Clinical Observations ◽  
Hypertrophic Scar Tissue ◽  
Indications For Use

For the past 3 years, Nottingham City Hospital Burns and Plastic Surgery Unit have been using silicone gel in addition to, or as an alternative to, accepted methods of treating hypertrophic scarring. This paper outlines the history, properties and indications for use of silicone gel. Its findings result from a retrospective study based largely on clinical observations.

In vitro study of ethosome penetration in human skin and hypertrophic scar tissue

2012 ◽  
Vol 8 (6) ◽  
pp. 1026-1033 ◽  
Author(s):  
Zhen Zhang ◽  
Yan Wo ◽  
Yixin Zhang ◽  
Danru Wang ◽  
Rong He ◽  
...  
Keyword(s):  
Human Skin ◽  
Hypertrophic Scar ◽  
In Vitro Study ◽  
Scar Tissue ◽  
Vitro Study ◽  
Hypertrophic Scar Tissue

Enhanced expression of insulin-like growth factor II is not a necessary event in Wilms' Tumour progression

1987 ◽  
Vol 8 (6) ◽  
pp. 865-868 ◽  
Author(s):  
Melissa H. Little ◽  
Gary Ablett ◽  
Peter J. Smith
Keyword(s):  
Growth Factor ◽  
Tumour Progression ◽  
Insulin Like Growth Factor ◽  
Wilms Tumour ◽  
Factor Ii ◽  
Enhanced Expression

scholarly journals Collagenase Production Is Lower in Post-Burn Hypertrophic Scar Fibroblasts Than in Normal Fibroblasts and Is Reduced by Insulin-Like Growth Factor-1

1996 ◽  
Vol 106 (3) ◽  
pp. 476-481 ◽  
Author(s):  
Aziz Ghahary ◽  
You J. Shen ◽  
Bernadette Nedelec ◽  
Rijian Wang ◽  
Paul G. Scott ◽  
...  
Keyword(s):  
Growth Factor ◽  
Hypertrophic Scar ◽  
Insulin Like Growth Factor

Reduced collagenase gene expression in fibroblasts from hypertrophic scar tissue

1996 ◽  
Vol 134 (5) ◽  
pp. 863-868 ◽  
Author(s):  
M. ARAKAWA ◽  
A. HATMOCHI ◽  
Y. MORI ◽  
K. MORI ◽  
H. UEKI ◽  
...  
Keyword(s):  
Gene Expression ◽  
Hypertrophic Scar ◽  
Scar Tissue ◽  
Hypertrophic Scar Tissue ◽  
Collagenase Gene

scholarly journals High-Efficiency Expression of TAT-bFGF Fusion Protein in Escherichia coli and the Effect on Hypertrophic Scar Tissue

PLoS ONE ◽  
2015 ◽  
Vol 10 (2) ◽  
pp. e0117448 ◽  
Author(s):  
Xuechao Jia ◽  
Haishan Tian ◽  
Lu Tang ◽  
Long Zheng ◽  
Lulu Zheng ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Fusion Protein ◽  
Hypertrophic Scar ◽  
High Efficiency ◽  
Scar Tissue ◽  
Hypertrophic Scar Tissue
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