Capillary chromatographic separation of mixtures of organic compounds using steam as the mobile phase

1975 ◽  
Vol 24 (10) ◽  
pp. 2239-2240 ◽  
Author(s):  
V. G. Berezkin ◽  
B. A. Rudenko ◽  
�. A. Kyazimov ◽  
M. N. Agaeva ◽  
A. A. Rodionov ◽  
...  
Keyword(s):  
Organic Compounds ◽  
Chromatographic Separation ◽  
Mobile Phase ◽  
Separation Of Mixtures

Biogenic brominated and iodinated organic compounds in ponds on the McMurdo Ice Shelf, Antarctica

1996 ◽  
Vol 8 (1) ◽  
pp. 45-48 ◽  
Author(s):  
Christian Schall ◽  
Klaus Gustav Heumann ◽  
Stephen De Mora ◽  
Peter A. Lee
Keyword(s):  
Distribution Pattern ◽  
Organic Compounds ◽  
Chromatographic Separation ◽  
Aquatic Systems ◽  
Biogenic Substance ◽  
Ice Shelf ◽  
Volatile Hydrocarbons ◽  
First Time ◽  
Gas Chromatographic Separation ◽  
Biogenic Compound

During January 1994 seven meltwater ponds on the McMurdo Ice Shelf were investigated for their content of biogenic iodinated and brominated volatile hydrocarbons. An efficient purge and trap system in combination with a powerful gas chromatographic separation and an electron capture detector achieved detection limits of 0.02–0.4 ng 1−1, depending on the different substances. The following compounds could be identified and quantified: CH3I, CH2I2, CH2C1I, CHBr3, CH2Br2, BrCH2CH2Br, CHBr2Cl, and CHBrCl2. This is the first time that 1,2–dibromoethane has been detected as a biogenic substance in the environment. In contrast to many other aquatic systems, where CH3I is found to be the most volatile iodine compound, CH2I2 showed the highest concentration in all ponds falling in the range of 5–20 ng 1−1. In three of seven ponds investigated, CH2CII was the second abundant iodinated substance. CHBr3 usually exhibited concentrations in the range of 2.5–8.6 ng 1−1. BrCH2CH2Br, previously not observed as a biogenic compound, was found to have concentrations similar to those of bromoform and even exceeded the bromoform content in two ponds and the CH2Br2 content in all ponds. Whether cyanobacteria, the dominant organisms in the ponds, are responsible for this distribution pattern must be clarified by further investigations.

scholarly journals Analysis of alkaloids from different chemical groups by different liquid chromatography methods

2012 ◽  
Vol 10 (3) ◽  
pp. 802-835 ◽  
Author(s):  
Anna Petruczynik
Keyword(s):  
Liquid Chromatography ◽  
Ion Exchange ◽  
Chromatographic Separation ◽  
Mobile Phase ◽  
Stationary Phases ◽  
Biologically Active ◽  
System Efficiency ◽  
Bonded Stationary Phases ◽  
Silica Alumina ◽  
Chemical Groups

AbstractAlkaloids are biologically active compounds widely used as pharmaceuticals and synthesised as secondary methabolites in plants. Many of these compounds are strongly toxic. Therefore, they are often subject of scientific interests and analysis. Since alkaloids — basic compounds appear in aqueous solutions as ionized and unionized forms, they are difficult for chromatographic separation for peak tailing, poor systems efficiency, poor separation and poor column-to-column reproducibility. For this reason it is necessity searching of more suitable chromatographic systems for analysis of the compounds. In this article we present an overview on the separation of selected alkaloids from different chemical groups by liquid chromatography thus indicating the range of useful methods now available for alkaloid analysis. Different selectivity, system efficiency and peaks shape may be achieved in different LC methods separations by use of alternative stationary phases: silica, alumina, chemically bonded stationary phases, cation exchange phases, or by varying nonaqueous or aqueous mobile phase (containing different modifier, different buffers at different pH, ion-pairing or silanol blocker reagents). Developments in TLC (NP and RP systems), HPLC (NP, RP, HILIC, ion-exchange) are presented and the advantages of each method for alkaloids analysis are discussed.

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