Transfer of D-isoascorbic acid and L-ascorbic acid into guinea pig eyes

1969 ◽  
Vol 26 (1) ◽  
pp. 164-170 ◽  
Author(s):  
Erik Linnér ◽  
Karin Nordström
Keyword(s):  
Ascorbic Acid ◽  
Guinea Pig ◽  
Isoascorbic Acid

Differential determination of D-isoascorbic acid and L-ascorbic acid in guinea pig organs

1969 ◽  
Vol 47 (4) ◽  
pp. 449-453 ◽  
Author(s):  
Omer Pelletier
Keyword(s):  
Ascorbic Acid ◽  
Guinea Pig ◽  
Guinea Pigs ◽  
Synthetic Diet ◽  
Isoascorbic Acid

To test the theory that D-isoascorbic acid (IAA) was not retained by the organs of animals, guinea pigs were fed a synthetic diet containing ascorbic acid (AA) plus IAA. The incorporation of IAA and AA was determined by making use of the different rates of osazone formation of oxidized AA and IAA with 2,4-dinitrophenylhydrazine. It was found that the organs of the guinea pigs retained a significant quantity of IAA which replaced a corresponding quantity of AA. The incorporated IAA could, in turn, be replaced by AA when only AA was subsequently given in the diet.

The retention of ascorbic acid by guinea-pig tissues

1971 ◽  
Vol 26 (3) ◽  
pp. 433-438 ◽  
Author(s):  
R. E. Hughes ◽  
R. J. Hurley ◽  
P. R. Jones
Keyword(s):  
Ascorbic Acid ◽  
Guinea Pig ◽  
Guinea Pigs ◽  
Aqueous Humour ◽  
Adrenal Glands ◽  
Eye Lens ◽  
Tissue Concentrations ◽  
Male And Female ◽  
Isoascorbic Acid ◽  
The Brain

1. Male and female guinea-pigs with high tissue concentrations of L-xyloascorbic acid (ascorbic acid, AA) and male guinea-pigs with high tissue concentrations of D-araboascorbic acid (isoascorbic acid, isoAA) were given a scorbutogenic diet and the rates of depletino from various tissues were measured fro both isomers.2. The loss of AA from the brain and eye lens was considerably less rapid than the loss from the adrenal glands, spleen and aqueous humour. After 14nd on the AA-free diet the AA concentrations in the brain and eye lens were 24 and 27% respectively of the initial concentrations; the corresponding values for the aqueous humour, adrenal glands and spleen were 3,4 and 5%. There was no apparent sex difference in the rate of loss of AA.3. The loss of isoAA was much more rapid than that of AA in the spleen, adrenal glands and aqueous humour; in the brain and eye lens the depletion patterns of the two isomers were similar.

Vitamin C activity of D-isoascorbic acid for the guinea pig

1969 ◽  
Vol 47 (12) ◽  
pp. 985-991 ◽  
Author(s):  
Omer Pelletier ◽  
Claude Godin
Keyword(s):  
Ascorbic Acid ◽  
Vitamin C ◽  
Guinea Pig ◽  
Small Proportion ◽  
Acid Content ◽  
Guinea Pigs ◽  
Ascorbic Acid Content ◽  
Similar Response ◽  
Synthetic Diet ◽  
Isoascorbic Acid

Guinea pigs fed with a synthetic diet or a rabbit ration were depleted of vitamin C for 17 days. The scorbutic animals were treated daily during 2 months with 40 mg D-isoascorbic acid (IAA) or 2 mg L-ascorbic acid (AA). Both isomers restored the growth of the animals and caused the disappearance of scorbutic symptoms. For 3 weeks, the two diets gave similar response, after which the animals fed with the rabbit ration grew much less. Animals given IAA ate less and had smaller weight gains than those given AA; this effect was overcome by pair-feeding. Only a small proportion of administered IAA was recovered in the organs and in the urine. The total ascorbic acid content (AA and IAA) of the IAA-treated animals was less than that of the AA-treated animals. The low AA content of the organs of the IAA-treated animals indicated that IAA had no significant sparing action on AA.

scholarly journals The uptake of D-araboascorbic acid (D-isoascorbic acid) by guinea-pig tissues

1969 ◽  
Vol 23 (2) ◽  
pp. 211-216 ◽  
Author(s):  
R. E. Hughes ◽  
R. J. Hurley
Keyword(s):  
Ascorbic Acid ◽  
Body Weight ◽  
Guinea Pig ◽  
Paper Chromatography ◽  
Guinea Pigs ◽  
Intestinal Tract ◽  
No Conversion ◽  
Isoascorbic Acid ◽  
Gastro Intestinal Tract ◽  
Oral Supplement

1. Guinea-pigs on a low, controlled intake of ascorbic acid (0.3 mg/100 g body-weight) were given daily an oral supplement of L-ascorbic acid (AA) or of D-isoascorbic acid (isoAA) (both 1.5 mg/100 g body-weight). The AA was deposited in the tissues, the isoAA was not.2. Intramuscularly administered isoAA was deposited in the tissues but not to the same extent as equivalent amounts of intramuscularly administered AA.3. Paper chromatography confirmed that intramuscularly administered isoAA was deposited in the tissues as isoAA, i.e. there was no conversion into AA.4. It is concluded that in guinea-pigs (a)AA is absorbed from the gastro-intestinal tract much more readily than isoAA and (b)AA is either more readily abstracted from the blood or is more readily retained by the tissues or both, than is isoAA.

scholarly journals METABOLISM OF l-ASCORBIC ACID, DEHYDRO-l-ASCORBIC ACID, AND DIKETO-l-GULONIC ACID IN THE GUINEA PIG

1952 ◽  
Vol 195 (2) ◽  
pp. 599-606 ◽  
Author(s):  
Charlotte M. Damron ◽  
Mary.Mills. Monier ◽  
Joseph H. Roe
Keyword(s):  
Ascorbic Acid ◽  
Guinea Pig
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