Differential determination of D-isoascorbic acid and L-ascorbic acid in guinea pig organs

1969 ◽  
Vol 47 (4) ◽  
pp. 449-453 ◽  
Author(s):  
Omer Pelletier
Keyword(s):  
Ascorbic Acid ◽  
Guinea Pig ◽  
Guinea Pigs ◽  
Synthetic Diet ◽  
Isoascorbic Acid

To test the theory that D-isoascorbic acid (IAA) was not retained by the organs of animals, guinea pigs were fed a synthetic diet containing ascorbic acid (AA) plus IAA. The incorporation of IAA and AA was determined by making use of the different rates of osazone formation of oxidized AA and IAA with 2,4-dinitrophenylhydrazine. It was found that the organs of the guinea pigs retained a significant quantity of IAA which replaced a corresponding quantity of AA. The incorporated IAA could, in turn, be replaced by AA when only AA was subsequently given in the diet.

Vitamin C activity of D-isoascorbic acid for the guinea pig

1969 ◽  
Vol 47 (12) ◽  
pp. 985-991 ◽  
Author(s):  
Omer Pelletier ◽  
Claude Godin
Keyword(s):  
Ascorbic Acid ◽  
Vitamin C ◽  
Guinea Pig ◽  
Small Proportion ◽  
Acid Content ◽  
Guinea Pigs ◽  
Ascorbic Acid Content ◽  
Similar Response ◽  
Synthetic Diet ◽  
Isoascorbic Acid

Guinea pigs fed with a synthetic diet or a rabbit ration were depleted of vitamin C for 17 days. The scorbutic animals were treated daily during 2 months with 40 mg D-isoascorbic acid (IAA) or 2 mg L-ascorbic acid (AA). Both isomers restored the growth of the animals and caused the disappearance of scorbutic symptoms. For 3 weeks, the two diets gave similar response, after which the animals fed with the rabbit ration grew much less. Animals given IAA ate less and had smaller weight gains than those given AA; this effect was overcome by pair-feeding. Only a small proportion of administered IAA was recovered in the organs and in the urine. The total ascorbic acid content (AA and IAA) of the IAA-treated animals was less than that of the AA-treated animals. The low AA content of the organs of the IAA-treated animals indicated that IAA had no significant sparing action on AA.

The retention of ascorbic acid by guinea-pig tissues

1971 ◽  
Vol 26 (3) ◽  
pp. 433-438 ◽  
Author(s):  
R. E. Hughes ◽  
R. J. Hurley ◽  
P. R. Jones
Keyword(s):  
Ascorbic Acid ◽  
Guinea Pig ◽  
Guinea Pigs ◽  
Aqueous Humour ◽  
Adrenal Glands ◽  
Eye Lens ◽  
Tissue Concentrations ◽  
Male And Female ◽  
Isoascorbic Acid ◽  
The Brain

1. Male and female guinea-pigs with high tissue concentrations of L-xyloascorbic acid (ascorbic acid, AA) and male guinea-pigs with high tissue concentrations of D-araboascorbic acid (isoascorbic acid, isoAA) were given a scorbutogenic diet and the rates of depletino from various tissues were measured fro both isomers.2. The loss of AA from the brain and eye lens was considerably less rapid than the loss from the adrenal glands, spleen and aqueous humour. After 14nd on the AA-free diet the AA concentrations in the brain and eye lens were 24 and 27% respectively of the initial concentrations; the corresponding values for the aqueous humour, adrenal glands and spleen were 3,4 and 5%. There was no apparent sex difference in the rate of loss of AA.3. The loss of isoAA was much more rapid than that of AA in the spleen, adrenal glands and aqueous humour; in the brain and eye lens the depletion patterns of the two isomers were similar.

scholarly journals The uptake of D-araboascorbic acid (D-isoascorbic acid) by guinea-pig tissues

1969 ◽  
Vol 23 (2) ◽  
pp. 211-216 ◽  
Author(s):  
R. E. Hughes ◽  
R. J. Hurley
Keyword(s):  
Ascorbic Acid ◽  
Body Weight ◽  
Guinea Pig ◽  
Paper Chromatography ◽  
Guinea Pigs ◽  
Intestinal Tract ◽  
No Conversion ◽  
Isoascorbic Acid ◽  
Gastro Intestinal Tract ◽  
Oral Supplement

1. Guinea-pigs on a low, controlled intake of ascorbic acid (0.3 mg/100 g body-weight) were given daily an oral supplement of L-ascorbic acid (AA) or of D-isoascorbic acid (isoAA) (both 1.5 mg/100 g body-weight). The AA was deposited in the tissues, the isoAA was not.2. Intramuscularly administered isoAA was deposited in the tissues but not to the same extent as equivalent amounts of intramuscularly administered AA.3. Paper chromatography confirmed that intramuscularly administered isoAA was deposited in the tissues as isoAA, i.e. there was no conversion into AA.4. It is concluded that in guinea-pigs (a)AA is absorbed from the gastro-intestinal tract much more readily than isoAA and (b)AA is either more readily abstracted from the blood or is more readily retained by the tissues or both, than is isoAA.

EFFECT OF HISTAMINE ON GASTRIC PEPSINOGEN, PEPSIN SECRETION, AND BLOOD PEPSINOGEN IN GUINEA PIGS

1957 ◽  
Vol 35 (1) ◽  
pp. 729-732
Author(s):  
K. Kowalewski
Keyword(s):  
Body Weight ◽  
Gastric Mucosa ◽  
Guinea Pig ◽  
Gastric Juice ◽  
Guinea Pigs ◽  
Gastric Tissue ◽  
Pepsin Secretion ◽  
Enzymatic Function ◽  
Guinea Pig Stomach

The endocrine and exocrine activity of guinea pig stomach was measured by the determination of pepsinogen in gastric tissue and in plasma. Gastric juice pepsin was also studied.A significant increase of both pepsinogen and pepsin was found in animals treated with a dose of histamine (75 mg. per kg. of body weight). These results give further evidence that the zymogenic cells of gastric mucosa may be stimulated by histamine. The determination of pepsinogen in gastric tissue seems to permit a direct approach to the enzymatic function of zymogenic cells.

Determination of ascorbic acid, dehydroascorbic acid, and isoascorbic acid in blood

1986 ◽  
Vol 325 (5) ◽  
pp. 473-475 ◽  
Author(s):  
Jörg Schreiber ◽  
Wolfgang Lohmann ◽  
Dietrich Unverzagt ◽  
Albert Otten
Keyword(s):  
Ascorbic Acid ◽  
Dehydroascorbic Acid ◽  
Isoascorbic Acid

scholarly journals Vitamin C status, serum cholesterol levels and bile composition in the pregnant guinea-pig

1980 ◽  
Vol 43 (1) ◽  
pp. 95-100 ◽  
Author(s):  
S. A. Jenkins
Keyword(s):  
Ascorbic Acid ◽  
Body Weight ◽  
Gall Bladder ◽  
Guinea Pig ◽  
Bile Acids ◽  
Serum Cholesterol ◽  
Guinea Pigs ◽  
Cholesterol Content ◽  
Biliary Secretion ◽  
Cholesterol Levels

1. Pregnant guinea-pigs receiving a low dose of L-ascorbic acid (0.2 mg/100 g body-weight per d) developed a hypercholesterolaemia in the third trimester of pregnancy, whereas no change in serum cholesterol levels was observed in pregnant animals receiving a higher dose of the vitamin (2 mg/100 g body-weight per d).2. Pregnancy in the group of guinea-pigs receiving the higher dose of L-ascorbic acid was associated with an increased biliary secretion of bile acids. No change was observed in the biliary secretion of bile acids in pregnant animals receiving the lower dose of L-ascorbic acid, but these animals secreted significantly more cholesterol.3. Changes in the biliary secretion of cholesterol and bile acids in the pregnant guinea-pig according to L-ascorbic acid intake were reflected in the composition of the gall-bladder bile. Thus, the gall-bladder bile of guinea-pigs receiving the lower dose of L-ascorbic acid contained more cholesterol, while the gall-bladder bile of those animals receiving the higher dose of the vitamin had a higher content of bile acids.4. The increased cholesterol content of the gall-bladder of pregnant guinea-pigs receiving the lower dose of L-ascorbic acid resulted in decreased bile acid:cholesterol and phospholipid: cholesterol values, conditions predisposing to cholelithiasis.

Transfer of D-isoascorbic acid and L-ascorbic acid into guinea pig eyes

1969 ◽  
Vol 26 (1) ◽  
pp. 164-170 ◽  
Author(s):  
Erik Linnér ◽  
Karin Nordström
Keyword(s):  
Ascorbic Acid ◽  
Guinea Pig ◽  
Isoascorbic Acid

INTERRELATIONSHIP OF VITAMINS A AND C ON TISSUE UBIQUINONES AND STEROLS OF RATS AND GUINEA PIGS

1967 ◽  
Vol 45 (6) ◽  
pp. 749-756 ◽  
Author(s):  
W. E. J. Phillips
Keyword(s):  
Ascorbic Acid ◽  
Vitamin A ◽  
Vitamin C ◽  
Guinea Pig ◽  
Guinea Pigs ◽  
Vitamin A Deficiency ◽  
Secondary Effect ◽  
Vitamin C Deficiency ◽  
Vitamins A And C ◽  
Combined Deficiency

The effect of administration of ascorbic acid to normal or vitamin A-deficient rats was studied in relation to hepatic levels of ubiquinones and sterols. Similar studies were made on tissues from guinea pigs deficient in vitamin C, vitamin A, or both. Vitamin A deficiency increased the concentration of liver ubiquinones in the rat. Administration of ascorbate did not influence tissue levels of ubiquinones or sterols. Vitamin C deficiency increased the concentration of sterols but not of ubiquinones in the liver of the guinea pig. Vitamin A deficiency did not increase ubiquinones nor did a combined deficiency of vitamins A and C. A secondary effect of vitamin C deficiency in the vitamin A-deficient rat is not the cause of increased ubiquinone levels.

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