Enzyme activities of monoamine oxidase, cathechol-o-methyltransferase and ?-aminoubutyric acid transaminase in primary astroglial cultures and adult rat brain from different brain regions

1984 ◽  
Vol 9 (1) ◽  
pp. 45-57 ◽  
Author(s):  
Elisabeth Hansson
Keyword(s):  
Monoamine Oxidase ◽  
Rat Brain ◽  
Enzyme Activities ◽  
Brain Regions ◽  
Adult Rat ◽  
Adult Rat Brain

Development of Monoamine Oxidase and Aldehyde Dehydrogenase in Reaggregating Cultures of Fetal Rat Brain Cells

1989 ◽  
Vol 16 (3) ◽  
pp. 281-286
Author(s):  
Olof Tottmar ◽  
Maria Söderbäck ◽  
Anders Aspberg
Keyword(s):  
Monoamine Oxidase ◽  
Rat Brain ◽  
Aldehyde Dehydrogenase ◽  
Brain Cells ◽  
Mao B ◽  
Adult Rat ◽  
Fetal Rat ◽  
Adult Rat Brain ◽  
Mao A ◽  
Fetal Rat Brain

The development of monoamine oxidase (MAO) and aldehyde dehydrogenase (ALDH) in reaggregation cultures of fetal rat brain cells was compared with that of enzymatic markers for glial and neuronal cells. Only MAO-A was detected in the cultures during the first week, but, during the following three weeks, the activity of MAO-B increased more rapidly than that of MAO-A. The ratio MAO-A/MAO-B in four-week aggregates was close to that found in the adult rat brain. The activity of ALDH started to increase rapidly after 15 days, and the developmental pattern was intermediate to those of the glial and neuronal markers. The activity after four weeks was close to that found in the adult rat brain. Epidermal growth factor (EGF) caused a slight decrease in the activities of the low-Km ALDH (after four weeks) and the neuronal marker, choline acetyltransferase (after two weeks), whereas the other markers were not affected. By contrast, the activities of MAO-A and MAO-B were greatly increased during almost the entire culture period. It is suggested that this effect of EGF was the result of increased mitotic activity and/or biochemical differentiation of other cell types present in the cell aggregates, e.g. capillary endothelial cells.

scholarly journals Effects of low and high deprenyl dose on antioxidant enzyme activities in the adult rat brain

2017 ◽  
Vol 36 (01) ◽  
pp. 83-90 ◽  
Author(s):  
Maria Lalkovicova ◽  
Frantiska Horvathova ◽  
Igor Sulla ◽  
Jozef Mihalik ◽  
Viera Danielisova
Keyword(s):  
Rat Brain ◽  
Antioxidant Enzyme ◽  
Enzyme Activities ◽  
Antioxidant Enzyme Activities ◽  
Adult Rat ◽  
Adult Rat Brain

Reduced Mg2+- ATPase Activity in the Hypoglycemic Adult Rat Brain

2001 ◽  
Vol 56 (9-10) ◽  
pp. 912-914 ◽  
Author(s):  
Stylianos Tsakiris ◽  
Christos Koromilas ◽  
Kleopatra H. Schulpis
Keyword(s):  
Atpase Activity ◽  
Rat Brain ◽  
Adult Male ◽  
Enzyme Activities ◽  
Male Rat ◽  
Whole Brain ◽  
Adult Rat ◽  
Adult Rat Brain ◽  
Adult Male Rat

Abstract The effects of different a-D-Glucose (Glu) concentrations (0 -16 mᴍ) on Na+, K+-ATPase and Mg2+-ATPase activities were investigated in homogenates of adult male rat whole brain at 37 °C. The enzyme activities were determined after 1h preincubation with Glu. Brain Na+, K+-ATPase was not affected by Glu different concentrations. On the contrary, Mg2+-ATPase activity was considerably reduced with Glu concentrations lower than 4 mᴍ. The enzyme was inhibited 40%, 50% or 80% with 3, 2 or 1 mᴍ of Glu, respectively. The above results suggest: a) The various concentrations of Glu have no effect on brain Na+, K+-ATPase activity, b) The inhibited brain Mg2+-ATPase in hypoglycemia produces low intracellular Mg2+, which could modulate the activity of Mg2+ -dependent enzymes and the rates of protein syn­thesis and growth of the cell.

scholarly journals Monoubiquitination and Cellular Distribution of XIAP in Neurons after Traumatic Brain Injury

2003 ◽  
Vol 23 (10) ◽  
pp. 1129-1136 ◽  
Author(s):  
George Lotocki ◽  
Ofelia F Alonso ◽  
Beata Frydel ◽  
W Dalton Dietrich ◽  
Robert W Keane
Keyword(s):  
Traumatic Brain Injury ◽  
Brain Injury ◽  
Rat Brain ◽  
Brain Regions ◽  
Normal Adult ◽  
Normal Brain ◽  
Adult Rat ◽  
Moderate Traumatic Brain Injury ◽  
Adult Rat Brain ◽  
Specific Regulation

XIAP is a member of the inhibitor of apoptosis (IAP) gene family that, in addition to suppressing cell death by inhibition and polyubiquitination of caspases, is involved in an increasing number of signaling cascades. Moreover, the function and regulation of XIAP in the central nervous system (CNS) is poorly understood. In this study, the authors investigated the cell-type expression, the subcellular distribution, ubiquitination of XIAP, and levels of Smac/DIABLO in the normal adult rat brain and in brains subjected to moderate traumatic brain injury (TBI). In the normal brain, XIAP was predominantly expressed in the perinuclear region of neurons. Traumatized brains showed dramatic alterations in cellular and regional expression of XIAP early after injury. Stereologic analyses of the number of XIAP-positive cells within the hippocampus of both hemispheres showed a biphasic response. Immunoprecipitation and immunoblots of extracts derived from different brain regions demonstrated that a single ubiquitin modifies XIAP. Normal cortex contained significantly higher levels of monoubiquitinated XIAP than hippocampus. TBI induced alterations in levels of monoubiquitinated XIAP that correlated with changes in XIAP distribution and immunoreactivity, suggesting that monoubiquitination of XIAP may be a regulator of XIAP location or activity. Similar levels of Smac/DIABLO were present in lysates of normal and traumatized brains. These data demonstrate for the first time a region-specific regulation of XIAP monoubiquitination in the normal adult rat brain, and after TBI, that may be a key event in the regulation of XIAP function contributing to the pathogenesis following injury.

Expression of neurocan after transient middle cerebral artery occlusion in adult rat brain

2005 ◽  
Vol 25 (1_suppl) ◽  
pp. S217-S217
Author(s):  
Kentaro Deguchi ◽  
Mikiro Takaishi ◽  
Takeshi Hayashi ◽  
Atsuhiko Oohira ◽  
Shoko Nagotani ◽  
...  
Keyword(s):  
Middle Cerebral Artery ◽  
Rat Brain ◽  
Middle Cerebral Artery Occlusion ◽  
Cerebral Artery ◽  
Artery Occlusion ◽  
Adult Rat ◽  
Adult Rat Brain ◽  
Cerebral Artery Occlusion
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