Electron flow shift inClostridium acetobutylicum fermentation by electrochemically introduced reducing equivalent

1988 ◽  
Vol 10 (2) ◽  
pp. 123-128 ◽  
Author(s):  
Tae Sung Kim ◽  
Byung Hong Kim

1985 ◽  
Vol 23 (12) ◽  
pp. 2785-2789
Author(s):  
S Akbar


2003 ◽  
Vol 775 ◽  
Author(s):  
Ivan Stanish ◽  
Daniel A. Lowy ◽  
Alok Singh

AbstractImmobilized polymerized electroactive vesicles (IPEVs) are submicron biocapsules capable of storing charge in confined environments and chemisorbing on surfaces. Methods to immobilize stable submicron sized electroactive vesicles and the means to measure electroactivity of IPEVs at nanolevels have been demonstrated. IPEVs can withstand steep potential gradients applied across their membrane, maintain their structural integrity against surfaces poised at high/low electrical potentials, retain electroactive material over several days, and reversibly mediate (within the membrane) electron flow between the electrode surface and vesicle interior. IPEVs have strong potential to be used for charge storage and electron coupling applications that operate on the submicron scale and smaller.



2012 ◽  
Vol 30 (1) ◽  
pp. 100
Author(s):  
Wei HUANG ◽  
Shi-Bao ZHANG ◽  
Kun-Fang CAO


2019 ◽  
Vol 99 (3) ◽  
Author(s):  
Egor I. Kiselev ◽  
Jörg Schmalian




Agriculture ◽  
2021 ◽  
Vol 11 (5) ◽  
pp. 401
Author(s):  
Minh Khiem Nguyen ◽  
Tin-Han Shih ◽  
Szu-Hsien Lin ◽  
Jun-Wei Lin ◽  
Hoang Chinh Nguyen ◽  
...  

Photosynthesis is an essential biological process and a key approach for raising crop yield. However, photosynthesis in rice is not fully investigated. This study reported the photosynthetic properties and transcriptomic profiles of chlorophyll (Chl) b-deficient mutant (ch11) and wild-type rice (Oryza sativa L.). Chl b-deficient rice revealed irregular chloroplast development (indistinct membranes, loss of starch granules, thinner grana, and numerous plastoglobuli). Next-generation sequencing approach application revealed that the differential expressed genes were related to photosynthesis machinery, Chl-biosynthesis, and degradation pathway in ch11. Two genes encoding PsbR (PSII core protein), FtsZ1, and PetH genes, were found to be down-regulated. The expression of the FtsZ1 and PetH genes resulted in disrupted chloroplast cell division and electron flow, respectively, consequently reducing Chl accumulation and the photosynthetic capacity of Chl b-deficient rice. Furthermore, this study found the up-regulated expression of the GluRS gene, whereas the POR gene was down-regulated in the Chl biosynthesis and degradation pathways. The results obtained from RT-qPCR analyses were generally consistent with those of transcription analysis, with the exception of the finding that MgCH genes were up-regulated which enhance the important intermediate products in the Mg branch of Chl biosynthesis. These results indicate a reduction in the accumulation of both Chl a and Chl b. This study suggested that a decline in Chl accumulation is caused by irregular chloroplast formation and down-regulation of POR genes; and Chl b might be degraded via the pheophorbide b pathway, which requires further elucidation.





2021 ◽  
Vol 14 (1) ◽  
Author(s):  
Hengkai Meng ◽  
Wei Zhang ◽  
Huawei Zhu ◽  
Fan Yang ◽  
Yanping Zhang ◽  
...  

Abstract Background An efficient supply of reducing equivalent is essential for chemicals production by engineered microbes. In phototrophic microbes, the NADPH generated from photosynthesis is the dominant form of reducing equivalent. However, most dehydrogenases prefer to utilize NADH as a cofactor. Thus, sufficient NADH supply is crucial to produce dehydrogenase-derived chemicals in cyanobacteria. Photosynthetic electron is the sole energy source and excess electrons are wasted in the light reactions of photosynthesis. Results Here we propose a novel strategy to direct the electrons to generate more ATP from light reactions to provide sufficient NADH for lactate production. To this end, we introduced an electron transport protein-encoding gene omcS into cyanobacterium Synechococcus elongatus UTEX 2973 and demonstrated that the introduced OmcS directs excess electrons from plastoquinone (PQ) to photosystem I (PSI) to stimulate cyclic electron transfer (CET). As a result, an approximately 30% increased intracellular ATP, 60% increased intracellular NADH concentrations and up to 60% increased biomass production with fourfold increased d-lactate production were achieved. Comparative transcriptome analysis showed upregulation of proteins involved in linear electron transfer (LET), CET, and downregulation of proteins involved in respiratory electron transfer (RET), giving hints to understand the increased levels of ATP and NADH. Conclusions This strategy provides a novel orthologous way to improve photosynthesis via enhancing CET and supply sufficient NADH for the photosynthetic production of chemicals.





Author(s):  
Zhakyp Karipbaeyev ◽  
Elena Polisadova ◽  
Aleksandr Ermolaev ◽  
Viktor Lisitsyn ◽  
Gulnur Alpyssova ◽  
...  


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