scholarly journals Transcription Profile Analysis of Chlorophyll Biosynthesis in Leaves of Wild-Type and Chlorophyll b-Deficient Rice (Oryza sativa L.)

Agriculture ◽  
2021 ◽  
Vol 11 (5) ◽  
pp. 401
Author(s):  
Minh Khiem Nguyen ◽  
Tin-Han Shih ◽  
Szu-Hsien Lin ◽  
Jun-Wei Lin ◽  
Hoang Chinh Nguyen ◽  
...  
Keyword(s):  
Oryza Sativa ◽  
Core Protein ◽  
Profile Analysis ◽  
Oryza Sativa L ◽  
Electron Flow ◽  
Chlorophyll Biosynthesis ◽  
Wild Type ◽  
Transcription Analysis ◽  
Chl A ◽  
Chl Biosynthesis

Photosynthesis is an essential biological process and a key approach for raising crop yield. However, photosynthesis in rice is not fully investigated. This study reported the photosynthetic properties and transcriptomic profiles of chlorophyll (Chl) b-deficient mutant (ch11) and wild-type rice (Oryza sativa L.). Chl b-deficient rice revealed irregular chloroplast development (indistinct membranes, loss of starch granules, thinner grana, and numerous plastoglobuli). Next-generation sequencing approach application revealed that the differential expressed genes were related to photosynthesis machinery, Chl-biosynthesis, and degradation pathway in ch11. Two genes encoding PsbR (PSII core protein), FtsZ1, and PetH genes, were found to be down-regulated. The expression of the FtsZ1 and PetH genes resulted in disrupted chloroplast cell division and electron flow, respectively, consequently reducing Chl accumulation and the photosynthetic capacity of Chl b-deficient rice. Furthermore, this study found the up-regulated expression of the GluRS gene, whereas the POR gene was down-regulated in the Chl biosynthesis and degradation pathways. The results obtained from RT-qPCR analyses were generally consistent with those of transcription analysis, with the exception of the finding that MgCH genes were up-regulated which enhance the important intermediate products in the Mg branch of Chl biosynthesis. These results indicate a reduction in the accumulation of both Chl a and Chl b. This study suggested that a decline in Chl accumulation is caused by irregular chloroplast formation and down-regulation of POR genes; and Chl b might be degraded via the pheophorbide b pathway, which requires further elucidation.

scholarly journals Temperature-mediated shifts in chlorophyll biosynthesis in leaves of chlorophyll b-lacking rice (Oryza sativa L.)

2021 ◽  
Vol 49 (2) ◽  
pp. 12306
Author(s):  
Khiem Minh NGUYEN ◽  
Zhi-Wei YANG ◽  
Tin-Han SHIH ◽  
Szu-Hsien LIN ◽  
Jun-Wei LIN ◽  
...  
Keyword(s):  
Crop Yields ◽  
Oryza Sativa L ◽  
Chlorophyll Biosynthesis ◽  
Differentially Expressed Gene ◽  
Growth And Yield ◽  
Light Harvesting Complex ◽  
Chl A ◽  
Light Harvesting Complex Ii ◽  
Influence Of Temperature On ◽  
Chl Biosynthesis

Extreme temperatures have become a threat to crop yields. To maintain plant growth and yield, chlorophyll (Chl) biosynthesis plays a crucial role in adaptation to temperature stress. This study investigated the influence of temperature on the biosynthesis and characteristics of pigments (Chl a, Chl b, and carotenoids) in the leaves of Chl b-lacking mutant rice (Chlorina 1, ch1) and wild-type rice (Norin No.8, wt). The ch1 showed thinner stacked grana caused by a decrease in thylakoid membranes per granum at 15 °C, whereas the destacked grana were observed at 35 °C after 12 h incubation. However, the grana are stacked normally, along with the absence of Chl b, and a significantly decreased amount of Chl a in both wt and ch1 were observed after heat stress exposure, demonstrating that light-harvesting complex II proteins are involved in grana stacking. Ch1 was sensitive to 15 °C during the first 4 h of incubation but it subsequently adapted to the cold environment. In addition, there were no significant differences in the photosynthesis between wt and ch1 after 12 h incubation at 35 °C. Differentially expressed gene (DEGs) analysis revealed that GluRS expression decreased, which resulted in a decline in Chl biosynthesis in wt and ch1 at 35 °C. At 8 h and 12 h, there were no significant differences in the expression of DEGs involved in Chl biosynthesis and degradation between wt and ch1 at 15 °C. ALAD expression in wt and ch1 at 15 °C decreased until it was undetectable. These findings suggested that ch1 may adapt to temperatures ranging from 15 °C to 35 °C.

scholarly journals Transcription analysis of chlorophyll biosynthesis in wildtype and chlorophyll b-lacking rice (Oryza sativa L.)

2020 ◽  
Vol 58 (3) ◽  
pp. 702-711 ◽  
Author(s):  
M.K. NGUYEN ◽  
T.H. SHIH ◽  
S.H. LIN ◽  
W.D HUANG ◽  
C.M. YANG
Keyword(s):  
Oryza Sativa ◽  
Oryza Sativa L ◽  
Chlorophyll Biosynthesis ◽  
Chlorophyll B ◽  
Transcription Analysis

Functional characterisation of OsAMT1.1 overexpression lines of rice, Oryza sativa

2006 ◽  
Vol 33 (4) ◽  
pp. 339 ◽  
Author(s):  
Anshuman Kumar ◽  
Brent N. Kaiser ◽  
M. Yaeesh Siddiqi ◽  
Anthony D. M. Glass
Keyword(s):  
Oryza Sativa ◽  
Plant Biomass ◽  
Oryza Sativa L ◽  
Wild Type ◽  
High Affinity ◽  
Functional Characterisation ◽  
Overexpression Line ◽  
Net Uptake ◽  
Transgenic Lines ◽  
Responsive Gene

In rice (Oryza sativa L.) OsAMT1.1 is the most active and / or most N-responsive gene responsible for high-affinity NH4+ transport (HATS) activity. We measured 13NH4+ influx and plant biomass in transgenic overexpression lines and two wild type cultivars of rice, Jarrah and Taipei, with one or more copies of OsAMT1.1. 13NH4+ influx was higher for the overexpression lines of Jarrah line when grown at 10 µm external NH4+ concentration, but not for the overexpression lines of Taipei. For seedlings grown at 2 mm external NH4+ concentration Jarrah lines 77-1 and 75-4 showed an increased influx; however, two overexpression lines of Taipei showed reduced influx rates. The biomasses of the transgenic lines grown at low and high external NH4+ concentrations were either reduced or showed no statistically significant differences compared with wild type lines. While 13NH4+ influx into roots of Jarrah line 75-4 grown at 10 µm external NH4+ concentration was significantly higher than in wild type, measurements of 13NH efflux revealed no differences, and thus net uptake of NH4+ was higher in this overexpression line.

CS3, a Ycf54 domain-containing protein, affects chlorophyll biosynthesis in rice (Oryza sativa L.)

Plant Science ◽  
2019 ◽  
Vol 283 ◽  
pp. 11-22 ◽  
Author(s):  
Ning Yu ◽  
Qunen Liu ◽  
Yingxin Zhang ◽  
Bo Zeng ◽  
Yuyu Chen ◽  
...  
Keyword(s):  
Oryza Sativa ◽  
Oryza Sativa L ◽  
Chlorophyll Biosynthesis

The effects of Cd on lipid peroxidation, hydrogen peroxide content and antioxidant enzyme activities in Cd-sensitive mutant rice seedlings

2007 ◽  
Vol 87 (1) ◽  
pp. 49-57 ◽  
Author(s):  
J. Chen ◽  
C. Zhu ◽  
D. Lin ◽  
Z. -X Sun
Keyword(s):  
Lipid Peroxidation ◽  
Oryza Sativa ◽  
Antioxidant Enzyme ◽  
Oryza Sativa L ◽  
Antioxidant Enzyme Activities ◽  
Wild Type ◽  
Rice Seedlings ◽  
Sensitive Mutant ◽  
Cd Toxicity ◽  
Potential Biomarker

Cadmium-sensitive rice (Oryza sativa L. subsp. Japonica ‘Zhonghua11’) mutants were obtained using an Agrobacterium tumefaciens-based gene delivery system. Significant phenotypic differences were observed between a Cd-sensitive mutant (ST) and wild type (WT) rice seedlings. Results indicated that Cd accumulation in the leaves of the mutant was twice that of the wild type after 10 d of 0.5 mM Cd2+ treatment. Furthermore, a rapid Cd-induced H2O2 increase was observed in the mutant leaves, which induced abnormally early activity in antioxidant enzymes such as superoxide dismutase (SOD). However, the mutant leaves showed lower catalase (CAT) activity. By contrast, guaiacol peroxidase (G-POD) activities were higher in the mutant than in the wild type roots. Together with the Cd toxicity-induced decline of early responsive enzymatic activities in vivo, especially CAT, the inability of mutants to scavenge accumulated H2O2 resulted in higher lipid peroxide levels. H2O2 might also strengthen the expression of G-POD as a signaling molecule. Results suggest that G-POD activity can be a potential biomarker reflecting Cd sensitivity in rice seedlings. Key words: Antioxidant enzyme, Cd toxicity, Cd-sensitive mutant, lipid peroxidation, rice (Oryza sativa L.)

scholarly journals Yellow-Leaf 1 encodes a magnesium-protoporphyrin IX monomethyl ester cyclase, involved in chlorophyll biosynthesis in rice (Oryza sativa L.)

PLoS ONE ◽  
2017 ◽  
Vol 12 (5) ◽  
pp. e0177989 ◽  
Author(s):  
Zhonghua Sheng ◽  
Yusong Lv ◽  
Wei Li ◽  
Rongjian Luo ◽  
Xiangjin Wei ◽  
...  
Keyword(s):  
Oryza Sativa ◽  
Oryza Sativa L ◽  
Chlorophyll Biosynthesis ◽  
Protoporphyrin Ix ◽  
Yellow Leaf ◽  
Monomethyl Ester
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