Hexamethylphosphoramide as analytical reagent: Isolation and characterization of Fe(SCN)3(HMPA)3 complex. gravimetric determination of iron

1984 ◽  
Vol 82 (5-6) ◽  
pp. 339-345
Author(s):  
P. Bruno ◽  
M. Caselli ◽  
A. Traini
Keyword(s):  
Isolation And Characterization ◽  
Gravimetric Determination ◽  
Analytical Reagent

scholarly journals Isolation and characterization of pathogenic Escherichia coli bacteriophages from chicken and beef offal

2020 ◽  
Vol 13 (1) ◽  
Author(s):  
Celosia Lukman ◽  
Christopher Yonathan ◽  
Stella Magdalena ◽  
Diana Elizabeth Waturangi
Keyword(s):  
Escherichia Coli ◽  
Foodborne Pathogens ◽  
High Efficiency ◽  
Multiplicity Of Infection ◽  
Isolation And Characterization ◽  
Transmission Electron ◽  
Pathogenic Escherichia Coli ◽  
Family Based

Abstract Objective This study was conducted to isolate and characterize lytic bacteriophages for pathogenic Escherichia coli from chicken and beef offal, and analyze their capability as biocontrol for several foodborne pathogens. Methods done in this research are bacteriophage isolation, purification, titer determination, application, determination of host range and minimum multiplicity of infection (miMOI), and bacteriophage morphology. Results Six bacteriophages successfully isolated from chicken and beef offal using EPEC and EHEC as host strain. Bacteriophage titers observed between 109 and 1010 PFU mL−1. CS EPEC and BL EHEC bacteriophage showed high efficiency in reduction of EPEC or EHEC contamination in meat about 99.20% and 99.04%. The lowest miMOI was 0.01 showed by CS EPEC bacteriophage. CI EPEC and BL EPEC bacteriophage suspected as Myoviridae family based on its micrograph from Transmission Electron Microscopy (TEM). Refers to their activity, bacteriophages isolated in this study have a great potential to be used as biocontrol against several foodborne pathogens.

Benzenephosphinic Acid as Analytical Reagent Gravimetric Determination of Ferric Iron

1957 ◽  
Vol 29 (1) ◽  
pp. 109-113 ◽  
Author(s):  
J. E. Banks ◽  
D. A. Skoog
Keyword(s):  
Ferric Iron ◽  
Gravimetric Determination ◽  
Analytical Reagent

Isolation and Characterization of a Chlorogenic Acid Esterase from Aspergillus niger

1980 ◽  
Vol 35 (3-4) ◽  
pp. 209-212 ◽  
Author(s):  
B. Schöbel ◽  
W. Pollmann
Keyword(s):  
Enzyme Activity ◽  
Chlorogenic Acid ◽  
Divalent Cations ◽  
Hydrolysis Product ◽  
The Other ◽  
Temperature Optimum ◽  
Pig Liver ◽  
Isolation And Characterization

Abstract The isolation and characterization of a specific chlorogenic acid esterase is described. The enzyme activity is measured by determination of the hydrolysis product caffeic acid. The enzyme had been concentrated by means of ultrafiltration and column-chromatography. The pH- and temperature optimum were 6.5 and 45 °C respectively. Divalent cations were not required for the enzyme activity. As other esterases, this enzyme is inhibited by di-isopropyl-phosphorofluoridate. The Km-value is 0.70 mᴍ chlorogenic acid, the molecular weight 240000. The described enzyme is specific for chlorogenic acid. On the other hand a typical unspecific esterase like the pig liver esterases does not split chloro­genic acid. The isoelectric focusing reveals several isoenzymes of chlorogenase within a pI-range of 4.0-4.5.

scholarly journals 2-Hydroxy-5-methylbenzophenone oxime(HMBO) as an Analytical Reagent for Gravimetric Determination of Cu(II)

2007 ◽  
Vol 4 (1) ◽  
pp. 97-102 ◽  
Author(s):  
S. K. Shingadia ◽  
K. K. Desai
Keyword(s):  
Copper Ion ◽  
Molar Absorptivity ◽  
Ratio Method ◽  
Formation Reaction ◽  
Gravimetric Determination ◽  
Analytical Reagent ◽  
Gibb’S Free Energy ◽  
The Stability ◽  
Job's Method

2-Hydroxy-5-methylbenzophenone oxime (HMBO) was developed as a new analytical reagent for the gravimetric determination of divalent copper ion. In pH rang of 3.0 to 6.0, the reagent gives a buff colored precipitate with Cu(II). Job’s method and mole ratio method revealed that the stoichiometry of the complex is 1:2 (metal: ligand). Beer’s law is obeyed up to 61.25 ppm of Cu(II). Molar absorptivity and Sandell’s sensitivity at 400 nm were found to be 6.32 × 102L mol-1cm-1and 0.359 μg/cm2respectively. The stability constant of Cu(II)-HMBO complex is found to be 2.43 × 109. Gibb’s free energy change for complex formation reaction was found to be -12.88 Kcal/mol. The reagent can be used for the analysis of brass and alloy.

Isolation and characterization of the Omp-PA porin from Porphyromonas asaccharolytica, determination of the omp-PA gene sequence and prediction of Omp-PA protein structure

Anaerobe ◽  
2007 ◽  
Vol 13 (2) ◽  
pp. 74-82 ◽  
Author(s):  
Lana Magalashvili ◽  
Izabella Pechatnikov ◽  
Hannah M. Wexler ◽  
Yeshayahu Nitzan
Keyword(s):  
Protein Structure ◽  
Gene Sequence ◽  
Isolation And Characterization

scholarly journals The Blocked Amino-Terminal Peptide of Feather Keratin

1971 ◽  
Vol 24 (1) ◽  
pp. 179 ◽  
Author(s):  
IJ O'donnell
Keyword(s):  
Molecular Weight ◽  
Acetic Acid ◽  
Amino Groups ◽  
Amino Terminal ◽  
Acetyl Content ◽  
Electrophoretic Patterns ◽  
Isolation And Characterization ◽  
Primary Amino

Proteins extracted from reduced and carboxymethylated feather keratins (SCM-keratins) have been studied by Harrap and Woods (1964a, 1964b, 1967). They have demonstrated the presence of electrophoretic heterogeneity amongst the proteins and have obtained a molecular weight of approximately 11,000 in agreement with earlier work of Woodin (1954). There was no indication of marked heterogeneity with respect to size. Using acid hydrolysis and determination of acetic acid produced they found an acetyl content of 1 �30 molesj104 g in the rachis off owl feathers. These were thought to be attached to primary amino groups since there were no O-acetyl groups. In the present paper the isolation and characterization of the predominant, and probably sole, amino-terminal tripeptide from goose feather calamus is described. Goose feather calamus was chosen because its extracted proteins had one of the simplest electrophoretic patterns of proteins from the feathers of a number of species (Harrap and Woods 1967).

scholarly journals 2-Hydroxy-4-n-propoxy-5-bromoacetophenone oxime as an Analytical Reagent for Gravimetric Determination of V(V)

2009 ◽  
Vol 6 (2) ◽  
pp. 303-307 ◽  
Author(s):  
Ambily P. Nair ◽  
J. Christine
Keyword(s):  
Molar Absorptivity ◽  
Ratio Method ◽  
Gravimetric Determination ◽  
Pentavalent Vanadium ◽  
Analytical Reagent ◽  
Gibb’S Free Energy ◽  
The Stability ◽  
Ph Range ◽  
Job's Method

2-Hydroxy-4-n-propoxy-5-bromoacetophenone oxime (HnPBAO) was developed as a new analytical reagent for the gravimetric determination of pentavalent vanadium ion. In the pH range 4.0-6.0, the reagent gave a brown coloured precipitate with V(V). Job’s method and Mole ratio method revealed that the stoichiometry of the complex is 1:1 (metal: ligand). Beer’s law is obeyed up to 20.38 ppm of V(V). Molar absorptivity and Sandells sensitivity at 450 nm were found to be 10.22 × 102L/mol/cm and 0.049 µg/cm2respectively. The stability constant of V(V)-HnPBAO complex is found to be 1.195 × 106. Gibb’s free energy change for complex formation reaction was found to be –8.34 kcal/mol. The reagent can be used for the analysis of vanadium in ferro-vanadium alloys.

In situ determination of kinetic parameters for biofilms: Isolation and characterization of oligotrophic biofilms

1986 ◽  
Vol 28 (11) ◽  
pp. 1753-1760 ◽  
Author(s):  
Bruce E. Rittmann ◽  
LouAnn Crawford ◽  
Cynthia K. Tuck ◽  
Eun Namkung
Keyword(s):  
Kinetic Parameters ◽  
Isolation And Characterization
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