Kinetics of the respiratory syncytial virus growth cycle in Hela cells

1969 ◽  
Vol 28 (2) ◽  
pp. 122-132 ◽  
Author(s):  
S. Levine ◽  
R. Hamilton
Keyword(s):  
Respiratory Syncytial Virus ◽  
Hela Cells ◽  
Growth Cycle ◽  
Virus Growth ◽  
Syncytial Virus ◽  
Kinetics Of

scholarly journals Chimeric Measles Virus (MV/RSV), Having Ectodomains of Respiratory Syncytial Virus (RSV) F and G Proteins Instead of Measles Envelope Proteins, Induced Protective Antibodies against RSV

Vaccines ◽  
2021 ◽  
Vol 9 (2) ◽  
pp. 156
Author(s):  
Akihito Sawada ◽  
Takashi Ito ◽  
Yoshiaki Yamaji ◽  
Tetsuo Nakayama
Keyword(s):  
Respiratory Syncytial Virus ◽  
G Proteins ◽  
Cell Lines ◽  
Measles Virus ◽  
Pathological Finding ◽  
Control Group ◽  
Protein Coding ◽  
Virus Growth ◽  
Protective Antibodies ◽  
Syncytial Virus

In our previous study, fusion (F) or glyco (G) protein coding sequence of respiratory syncytial virus (RSV) was inserted at the P/M junction of the measles AIK-C vector (MVAIK), and the recombinant measles virus induced protective immune responses. In the present study, the ectodomains of measles fusion (F) and hemagglutinin (HA) proteins were replaced with those of RSV F and G proteins, and a chimeric MV/RSV vaccine was developed. It expressed F and G proteins of RSV and induced cytopathic effect (CPE) in epithelial cell lines (Vero, A549, and HEp-2 cells), but not in lymphoid cell lines (B95a, Jurkat, and U937 cells). A chimeric MV/RSV grew similarly to AIK-C with no virus growth at 39 °C. It induced NT antibodies against RSV in cotton rats three weeks after immunization through intramuscular route and enhanced response was observed after the second dose at eight weeks. After the RSV challenge with 106 PFU, significantly lower virus (101.4±0.1 PFU of RSV) was recovered from lung tissue in the chimeric MV/RSV vaccine group than in the MVAIK control group with 104.6±0.2 PFU (p < 0.001) and no obvious inflammatory pathological finding was noted. The strategy of ectodomain replacement in the measles virus vector is expected to lead to the development of safe and effective vaccines for other enveloped viruses.

Determinants and kinetics of cytokine expression patterns in lungs of vaccinated mice challenged with respiratory syncytial virus

Vaccine ◽  
1997 ◽  
Vol 15 (6-7) ◽  
pp. 597-602 ◽  
Author(s):  
Yi-Wei Tang ◽  
Kathleen M. Neuzil ◽  
Julie E. Fischer ◽  
Frances W. Robinson ◽  
Robert A. Parker ◽  
...  
Keyword(s):  
Respiratory Syncytial Virus ◽  
Expression Patterns ◽  
Cytokine Expression ◽  
Syncytial Virus ◽  
Kinetics Of

scholarly journals Kinetics of antibody-induced modulation of respiratory syncytial virus antigens in a human epithelial cell line

2007 ◽  
Vol 4 (1) ◽  
pp. 68 ◽  
Author(s):  
Rosa E Sarmiento ◽  
Rocio G Tirado ◽  
Laura E Valverde ◽  
Beatriz Gómez-Garcia
Keyword(s):  
Respiratory Syncytial Virus ◽  
Epithelial Cell ◽  
Cell Line ◽  
Epithelial Cell Line ◽  
Human Epithelial Cell ◽  
Virus Antigens ◽  
Syncytial Virus ◽  
Kinetics Of

scholarly journals Effect of Coexpression of Interleukin-2 by Recombinant Respiratory Syncytial Virus on Virus Replication, Immunogenicity, and Production of Other Cytokines

2000 ◽  
Vol 74 (15) ◽  
pp. 7151-7157 ◽  
Author(s):  
Alexander Bukreyev ◽  
Stephen S. Whitehead ◽  
Calman Prussin ◽  
Brian R. Murphy ◽  
Peter L. Collins
Keyword(s):  
Respiratory Syncytial Virus ◽  
Mononuclear Cells ◽  
Protective Efficacy ◽  
Interleukin 2 ◽  
Cytokine Mrna ◽  
Virus Growth ◽  
Ifn Γ ◽  
Syncytial Virus

ABSTRACT We constructed rRSV/mIL-2, a recombinant respiratory syncytial virus (rRSV) containing the coding sequence of murine interleukin-2 (mIL-2) in a transcription cassette inserted into the G-F intergenic region. The recovered virus (rRSV/mIL-2) expressed high levels (up to 2.8 μg/ml) of mIL-2 in cell culture. Replication of rRSV/mIL-2 in vitro was reduced up to 13.6-fold from that of wild-type (wt) rRSV, an effect that was due to the presence of the foreign insert but was not specific to mIL-2. Replication of the rRSV/mIL-2 virus in the upper and lower respiratory tracts of BALB/c mice was reduced up to 6.3-fold, an effect that was specific to mIL-2. The antibody response, including the levels of RSV-specific serum immunoglobulin G1 (IgG1), IgG2a, IgA, and total IgG, and the level of protective efficacy against wt RSV challenge were not significantly different from those of wt rRSV. Analysis of total pulmonary cytokine mRNA isolated 1 and 4 days following infection with rRSV/mIL-2 revealed elevated levels of mRNA for IL-2, gamma interferon (IFN-γ), IL-4, IL-5, IL-6, IL-10, IL-13, and IL-12 p40 compared to those for wt rRSV. Flow cytometry of total pulmonary mononuclear cells isolated 10 days following infection with rRSV/mIL-2 revealed increased levels of CD4+ T lymphocytes expressing either IFN-γ or IL-4 compared to those of wt rRSV. These elevations in cytokine mRNA or cytokine-expressing CD4+cells relative to those of wt rRSV-primed animals were not observed following challenge with wt RSV on day 28. Thus, the expression of mIL-2 by rRSV was associated with a modest attenuation of virus growth in vivo, induction of serum antibodies at levels comparable to that of wt rRSV, and transient increases in both the Th1 and Th2 CD4+ lymphocytes and cytokine mRNAs compared to those of wt rRSV.

scholarly journals Seroepidemiologies of Human Metapneumovirus and Respiratory Syncytial Virus in Young Children, Determined with a New Recombinant Fusion Protein Enzyme-Linked Immunosorbent Assay

2013 ◽  
Vol 20 (10) ◽  
pp. 1654-1656 ◽  
Author(s):  
Sarah R. Dunn ◽  
Alex B. Ryder ◽  
Sharon J. Tollefson ◽  
Meng Xu ◽  
Benjamin R. Saville ◽  
...  
Keyword(s):  
Respiratory Syncytial Virus ◽  
Young Children ◽  
Fusion Protein ◽  
Human Metapneumovirus ◽  
Enzyme Linked Immunosorbent Assay ◽  
Significant Difference ◽  
Syncytial Virus ◽  
Antibody Decline ◽  
Kinetics Of ◽  
Protein Enzyme

ABSTRACTWe compared antibodies against human metapneumovirus (HMPV) and respiratory syncytial virus (RSV) in children. The antibody nadirs for both viruses were at 3 to 5 months, and the majority of children were seropositive for both by 2 years. There was no significant difference in the kinetics of maternal antibody decline or seroconversion relative to the two viruses.

scholarly journals 2′-5′-Oligoadenylate Synthetase-Like Protein Inhibits Respiratory Syncytial Virus Replication and Is Targeted by the Viral Nonstructural Protein 1

2015 ◽  
Vol 89 (19) ◽  
pp. 10115-10119 ◽  
Author(s):  
Jayeeta Dhar ◽  
Rolando A. Cuevas ◽  
Ramansu Goswami ◽  
Jianzhong Zhu ◽  
Saumendra N. Sarkar ◽  
...  
Keyword(s):  
Innate Immunity ◽  
Respiratory Syncytial Virus ◽  
Virus Replication ◽  
Nonstructural Protein ◽  
Oligoadenylate Synthetase ◽  
Antiviral Protein ◽  
Virus Growth ◽  
Nonstructural Protein 1 ◽  
Inhibitory Activities ◽  
Syncytial Virus

2′-5′-Oligoadenylate synthetase-like protein (OASL) is an interferon-inducible antiviral protein. Here we describe differential inhibitory activities of human OASL and the two mouse OASL homologs against respiratory syncytial virus (RSV) replication. Interestingly, nonstructural protein 1 (NS1) of RSV promoted proteasome-dependent degradation of specific OASL isoforms. We conclude that OASL acts as a cellular antiviral protein and that RSV NS1 suppresses this function to evade cellular innate immunity and allow virus growth.

scholarly journals Kinetics of Synthesis and Phosphorylation of Respiratory Syncytial Virus Polypeptides

1988 ◽  
Vol 69 (2) ◽  
pp. 313-323 ◽  
Author(s):  
D. M. Lambert ◽  
J. Hambor ◽  
M. Diebold ◽  
B. Galinski
Keyword(s):  
Respiratory Syncytial Virus ◽  
Syncytial Virus ◽  
Kinetics Of

scholarly journals Kinetics of Synthesis of Respiratory Syncytial Virus Glycoproteins

1985 ◽  
Vol 66 (9) ◽  
pp. 1983-1990 ◽  
Author(s):  
B. F. Fernie ◽  
G. Dapolito ◽  
P. J. Cote ◽  
J. L. Gerin
Keyword(s):  
Respiratory Syncytial Virus ◽  
Syncytial Virus ◽  
Kinetics Of
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