Location and size of carotid body-like organs (paraganglia) revealed in rats by the permeability of blood vessels to Evans blue dye

1981 ◽  
Vol 10 (4) ◽  
pp. 607-643 ◽  
Author(s):  
Donald M. McDonald ◽  
Richard W. Blewett
Keyword(s):  
Blood Vessels ◽  
Carotid Body ◽  
Evans Blue ◽  
Blue Dye ◽  
Evans Blue Dye

scholarly journals Injection of Evans blue dye to fluorescently label and image intact vasculature

BioTechniques ◽  
2020 ◽  
Author(s):  
Samuel E Honeycutt ◽  
Lori L O'Brien
Keyword(s):  
Fluorescence Microscopy ◽  
Blood Vessels ◽  
Evans Blue ◽  
Blue Dye ◽  
Vascular Networks ◽  
Fluorescent Properties ◽  
Evans Blue Dye ◽  
Health And Disease ◽  
Disease Understanding

Blood vessels perform critical functions in both health and disease. Understanding how vessels form, pattern and respond to damage is essential. However, labeling and imaging the vasculature to ascertain these properties can be difficult and time-consuming. Here, the authors present a novel methodology for rapidly and efficiently labeling whole vascular networks in vivo by exploiting the fluorescent properties of Evans blue. By combining the labeling with fluorescence microscopy, this method enables visualization of whole tissue vasculature for a fraction of the time and cost compared with traditional methods.

scholarly journals Evans Blue Dye as an in vivo marker of myofibre damage: optimising parameters for detecting initial myofibre membrane permeability

2002 ◽  
Vol 200 (1) ◽  
pp. 69-79 ◽  
Author(s):  
P. W. Hamer ◽  
J. M. McGeachie ◽  
M. J. Davies ◽  
M. D. Grounds
Keyword(s):  
Membrane Permeability ◽  
Evans Blue ◽  
Blue Dye ◽  
Evans Blue Dye

Regional Deposition of Inhaled Evans Blue Dye in Mechanically Ventilated Rabbits with Air or Helium Oxygen Mixture

1998 ◽  
Vol 24 (2) ◽  
pp. 159-172 ◽  
Author(s):  
Magnus Svartengren ◽  
Patrik Skogward ◽  
Ola Nerbrink ◽  
Magnus Dahlbäck
Keyword(s):  
Evans Blue ◽  
Oxygen Mixture ◽  
Blue Dye ◽  
Mechanically Ventilated ◽  
Evans Blue Dye ◽  
Regional Deposition

scholarly journals Acoustic enhanced Evans blue dye perfusion in neurological tissues

2007 ◽  
Author(s):  
George K. Lewis Jr. ◽  
Willam L. Olbricht ◽  
George Lewis
Keyword(s):  
Evans Blue ◽  
Blue Dye ◽  
Evans Blue Dye

Flow of edema fluid into pulmonary airways

1983 ◽  
Vol 55 (4) ◽  
pp. 1262-1268 ◽  
Author(s):  
G. R. Mason ◽  
R. M. Effros
Keyword(s):  
Evans Blue ◽  
Left Atrial ◽  
Blue Dye ◽  
Edema Formation ◽  
Evans Blue Dye ◽  
Colloid Particles ◽  
Edema Fluid ◽  
Pulmonary Airways ◽  
99Mtc Sulfur Colloid

An in situ rabbit preparation was used to characterize the manner in which edema fluid enters the airways when left atrial pressures are elevated. The airways were initially filled with fluid to minimize retrograde flow of edema fluid into the alveoli. The airway solution contained 125I-albumin and in some studies [14C]sucrose, and the lungs were perfused with a comparable solution which contained albumin labeled with Evans blue dye and 99mTc-diethylenetriaminepentaacetate (DTPA) or 99mTc-sulfur-colloid particles (0.4-1.7 micron diam). After 30 min of perfusion, fluid was pumped from the airways into serial tubes. When left atrial pressures were low, there was very little transfer of labels detectable between the airway and perfusate solutions. However when left atrial pressures were increased to either 15 or 22 cmH2O, fluid entered the airways containing approximately the same concentrations of Evans blue dye and 99mTc-DTPA as those present in the perfusate. In contrast, the concentration of colloid particles averaged less than 5% perfusate concentrations, indicating that the fluid had not escaped through a tear in the barriers separating the vascular and airway compartments. Concentrations of the perfusate fluid and indicators were highest in the initial samples pumped from the airways. These observations suggest that some of the fluid entering the airways may be derived from peribronchial cuffs or that there are marked regional differences in edema formation from alveoli.

A modified bioassay for heat-stable Escherichia coli enterotoxin

1977 ◽  
Vol 23 (3) ◽  
pp. 331-336 ◽  
Author(s):  
S. Stavric ◽  
D. Jeffrey
Keyword(s):  
Escherichia Coli ◽  
Body Weight ◽  
Incubation Time ◽  
Evans Blue ◽  
Room Temperature ◽  
Blue Dye ◽  
Evans Blue Dye ◽  
Heat Stable ◽  
Stomach Distention ◽  
Time Required

Infant mice were injected orally with preparations containing Escherichia coli heat-stable enterotoxin (ST) and Evans blue dye, and incubated at 22 °C. With enterotoxin-positive samples, the stomach was distended and contained essentially all of the dye. With enterotoxin-negative samples, the stomach remained normal in size and the dye passed freely into the intestines. The time required to obtain the maximum ratio of gut weight to body weight varied from 30 to 90 min and was dependent upon the concentration of enterotoxin. Heat-labile enterotoxin (LT) had no effect during this period.Based on these findings, the mouse incubation time was reduced from 4 h to 90 min, and the heating of test samples was retained only for confirmation of ST. The location of the dye and stomach distention served as an indicator of positive responses to ST. Incubation of the mice at room temperature (22 °C) was found satisfactory.

scholarly journals A Study of the Pathogenesis and Prevention of Central Pontine Myelinolysis in a Rat Model

2006 ◽  
Vol 34 (3) ◽  
pp. 264-271 ◽  
Author(s):  
Q-H Ke ◽  
T-B Liang ◽  
J Yu ◽  
S-S Zheng
Keyword(s):  
Hypertonic Saline ◽  
Blood Brain Barrier ◽  
Evans Blue ◽  
Central Pontine Myelinolysis ◽  
Brain Barrier ◽  
Blue Dye ◽  
Evans Blue Dye ◽  
Pontine Myelinolysis ◽  
Demyelinating Lesions ◽  
Central Pontine

The development of central pontine myelinolysis was studied in rats. Severe hyponatraemia was induced using vasopressin tannate and 2.5% dextrose in water and then rapidly corrected with hypertonic saline alone, hypertonic saline and dexamethasone simultaneously, or hypertonic saline plus dexamethasone 24 h later. The permeability of the blood-brain barrier was evaluated using the extravasation of Evans blue dye and the expression of inducible nitric oxide synthase (iNOS) in the brain was examined using Western blot analysis. Histological sections were examined for demyelinating lesions. In rats receiving hypertonic saline alone, Evans blue dye content and expression of iNOS began to increase 6 and 3 h, respectively, after rapid correction of hyponatraemia and demyelinating lesions were seen. When dexamethasone was given simultaneously with hypertonic saline, these increases were inhibited and demyelinating lesions were absent. These effects were lost if dexamethasone injection was delayed. Disruption of the blood-brain barrier and increased iNOS expression may be involved in the pathogenesis of central pontine myelinolysis, and early treatment with dexamethasone may help prevent the development of central pontine myelinolysis.

scholarly journals Suppression of tuberculoimmunity in mice by Evans blue dye.

1982 ◽  
Vol 38 (3) ◽  
pp. 932-937 ◽  
Author(s):  
A J Crowle ◽  
M H May
Keyword(s):  
Evans Blue ◽  
Blue Dye ◽  
Evans Blue Dye

99mTc–Evans Blue Dye for Mapping Contiguous Lymph Node Sequences and Discriminating the Sentinel Lymph Node in an Ovine Model

2006 ◽  
Vol 13 (5) ◽  
pp. 692-700 ◽  
Author(s):  
Chris Tsopelas ◽  
Elaine Bevington ◽  
James Kollias ◽  
Sabah Shibli ◽  
Gelareh Farshid ◽  
...  
Keyword(s):  
Lymph Node ◽  
Sentinel Lymph Node ◽  
Evans Blue ◽  
Blue Dye ◽  
Ovine Model ◽  
Evans Blue Dye
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