Nucleotide sequence of the coat protein coding region of the potyvirus tobacco vein-banding mosaic virus

1994 ◽  
Vol 138 (1-2) ◽  
pp. 17-25 ◽  
Author(s):  
B. -Y. Chang ◽  
C. R. Huang ◽  
S. -D. Yeh ◽  
J. -K. Chiang ◽  
L. -M. Hung ◽  
...  
Keyword(s):  
Nucleotide Sequence ◽  
Coat Protein ◽  
Mosaic Virus ◽  
Coding Region ◽  
Protein Coding

Sequence diversity in the coat protein coding region of the genome RNA of Johnsongrass mosaic virus in Australia

2004 ◽  
Vol 149 (8) ◽  
Author(s):  
H. K. C. Laidlaw ◽  
D. M. Persley ◽  
C. K. Pallaghy ◽  
I. D. Godwin
Keyword(s):  
Coat Protein ◽  
Mosaic Virus ◽  
Sequence Diversity ◽  
Coding Region ◽  
Protein Coding

scholarly journals Occurrence of Cucumber green mottle mosaic virus on Cucurbitaceous Plants in China

Plant Disease ◽  
2009 ◽  
Vol 93 (2) ◽  
pp. 200-200 ◽  
Author(s):  
Y. J. Zhang ◽  
G. F. Li ◽  
M. F. Li
Keyword(s):  
Nucleotide Sequence ◽  
Coat Protein ◽  
Mosaic Virus ◽  
Cucumis Sativus ◽  
Cucumis Melo ◽  
Citrullus Lanatus ◽  
Plant Production ◽  
Liaoning Province ◽  
Coding Region ◽  
Fruit Samples

Cucumber green mottle mosaic virus (CGMMV) was first discovered in 2003 in China (2) and developed an epidemic during 2005 (1). To know the occurrence scale and damage level of CGMMV, a survey was carried out in the main areas of cucurbitaceous plant production and seed trading in 2006 and 2007 in Mainland China. Samples of 739 plants of 16 types (seed samples from Cucurbita moschata, Cucumis melo, Lagenaria siceraria, Cucumis sativus, Momordica charantia, L. siceraria var. clavata, and C. pepo, leaf samples from C. moschata, Cucumis melo, L. siceraria, Cucumis sativus, and M. charantia, fruit samples from C. moschata, Citrullus lanatus, and L. siceraria, and seedling samples from Citrullus lanatus) were collected from 13 regions and analyzed by a double antibody sandwich (DAS)-ELISA. CGMMV was detected in Citrullus lanatus, L. siceraria, Cucumis melo, and C. moschata from six regions in 2006. Citrullus vulgaris, Cucumis melo, and C. moschata were infected most frequently; in 76, 60, and 30 of the leaf samples, respectively. CGMMV was tested positive in the samples of 8 seedlings, 23 seeds, and 1 fruit. In contrast, all samples tested negative in 2007. To confirm CGMMV identification, ELISA-positive samples were detected by reverse transcription-PCR assay using virus-specific primers that amplified a 524-bp fragment in the coat protein coding region. The nucleotide sequence of the PCR product (GenBank Accession No. DQ997778) isolated form L. siceraria in Liaoning Province (LHP) showed 100% identity with the Japanese watermelon strain (Japan W) and 91% identity with the Greece GR5 strain. On the basis of the symptoms of indicator plants (chlorotic spot and systemic mosaic were observed in L. siceraria, Chenopodium amaranticolor, and Cucumis sativus; no infection was observed in Datura stramonium, Nicotiana tabacum, and Chenopodium quinoa) and coat protein nucleotide sequence comparisons, the two CGMMV isolates of Wcn (1) and LHP from China should be grouped into the watermelon strain. The results showed that the government should establish effective quarantine strategy and the growers take proper planting measures to avoid further spreading of this virus. References: (1) H. Y. Chen et al. Acta Phytopathol. Sin. 36:306, 2006. (2) B. X. Qin et al. Plant Quarantine. 19:4, 2005.

Genetic diversity in the coat protein coding region of eighty-six sugarcane mosaic virus isolates from eight countries, particularly from Cameroon and Congo

2003 ◽  
Vol 148 (2) ◽  
pp. 357-372 ◽  
Author(s):  
O. M. Alegria ◽  
M. Royer ◽  
M. Bousalem ◽  
M. Chatenet ◽  
M. Peterschmitt ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Coat Protein ◽  
Mosaic Virus ◽  
Sugarcane Mosaic Virus ◽  
Coding Region ◽  
Protein Coding ◽  
Virus Isolates

Nucleotide sequence of the coat protein coding region of tulip breaking virus

Virus Genes ◽  
1994 ◽  
Vol 8 (2) ◽  
pp. 165-167 ◽  
Author(s):  
Kazuyuki Ohira ◽  
Shigetou Namba ◽  
Masamichi Miyagawa ◽  
Takaaki Kusumi ◽  
Tsuneo Tsuchizaki
Keyword(s):  
Nucleotide Sequence ◽  
Coat Protein ◽  
Coding Region ◽  
Protein Coding ◽  
Tulip Breaking Virus

scholarly journals The Infectivities of Turnip Yellow Mosaic Virus Genomes with Altered tRNA Mimicry Are Not Dependent on Compensating Mutations in the Viral Replication Protein

2000 ◽  
Vol 74 (18) ◽  
pp. 8368-8375 ◽  
Author(s):  
Sergei A. Filichkin ◽  
Kay L. Bransom ◽  
Joel B. Goodwin ◽  
Theo W. Dreher
Keyword(s):  
Mosaic Virus ◽  
Replication Protein ◽  
Yellow Mosaic Virus ◽  
Turnip Yellow Mosaic Virus ◽  
Wild Type ◽  
Coding Region ◽  
Protein Coding ◽  
Complete Sequencing ◽  
Strand Initiation ◽  
Virus Genomes

ABSTRACT Five highly infectious turnip yellow mosaic virus (TYMV) genomes with sequence changes in their 3′-terminal regions that result in altered aminoacylation and eEF1A binding have been studied. These genomes were derived from cloned parental RNAs of low infectivity by sequential passaging in plants. Three of these genomes that are incapable of aminoacylation have been reported previously (J. B. Goodwin, J. M. Skuzeski, and T. W. Dreher, Virology 230:113–124, 1997). We now demonstrate by subcloning the 3′ untranslated regions into wild-type TYMV RNA that the high infectivities and replication rates of these genomes compared to their progenitors are mostly due to a small number of mutations acquired in the 3′ tRNA-like structure during passaging. Mutations in other parts of the genome, including the replication protein coding region, are not required for high infectivity but probably do play a role in optimizing viral amplification and spread in plants. Two other TYMV RNA variants of suboptimal infectivities, one that accepts methionine instead of the usual valine and one that interacts less tightly with eEF1A, were sequentially passaged to produce highly infectious genomes. The improved infectivities of these RNAs were not associated with increased replication in protoplasts, and no mutations were acquired in their 3′ tRNA-like structures. Complete sequencing of one genome identified two mutations that result in amino acid changes in the movement protein gene, suggesting that improved infectivity may be a function of improved viral dissemination in plants. Our results show that the wild-type TYMV replication proteins are able to amplify genomes with 3′ termini of variable sequence and tRNA mimicry. These and previous results have led to a model in which the binding of eEF1A to the 3′ end to antagonize minus-strand initiation is a major role of the tRNA-like structure.

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